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110 条记录 (耗时 0.028 秒)

    Coccidiosis vaccines
    73.
    发明公开
    Coccidiosis vaccines 失效
    Vakzine gegen Coccidiose。

    公开(公告)号：EP0439056A2

    公开(公告)日：1991-07-31

    申请号：EP91100502.3

    申请日：1991-01-17

    申请人： F. HOFFMANN-LA ROCHE AG

    发明人： Binger, Mary-Helen

    IPC分类号： C12N15/30 ,  A61K39/012 ,  C07K13/00

    CPC分类号： C07K14/455 ,  A61K39/00

    摘要： The invention provides a DNA coding for a precursor to an Eimeria merozoite surface antigen, which surface antigen has an apparent molecular weight of about 23 kilodaltons by SDS PAGE. Based on this DNA proteins having one or more immunoreactive and/or antigenic determinants of the said surface antigen may be prepared as well as recombinant vectors and recombinant viruses containing the said DNA or framents thereof and transformed microorganisms containing such vectors and viruses. Thus, the present invention relates also to methods for producing the said proteins and the transformed microorganisms. The present case also relates to the purified 23 kDa merozoite surface antigen itself and the 30 kDa precursor form thereof per se and to methods for protecting poultry against coccidiosis using the Eimeria surface antigen, the precursor protein and/or fragments thereof. The proteins and fragments of the invention can be administered for such protection either as purified proteins or in the form of DNA encoding the protein in a suitable viral vector such as vaccinia virus.

    摘要翻译： 本发明提供了编码艾美球虫裂殖子表面抗原前体的DNA，该表面抗原通过SDS PAGE具有约23千道尔顿的表观分子量。 基于该DNA可以制备具有所述表面抗原的一种或多种免疫反应性和/或抗原决定簇的蛋白质，以及含有所述DNA或其构象的重组载体和重组病毒以及含有该载体和病毒的转化微生物。 因此，本发明还涉及生产所述蛋白质和转化的微生物的方法。 本案还涉及纯化的23kDa裂殖子表面抗原本身及其30kDa前体形式本身以及使用艾美球虫表面抗原，前体蛋白和/或其片段保护家禽抗球虫病的方法。 本发明的蛋白质和片段可以作为纯化的蛋白质或以合适的病毒载体如牛痘病毒编码蛋白质的DNA的形式进行这种保护。

    Eimeria membrane associated protein immunogens
    75.
    发明公开
    Eimeria membrane associated protein immunogens 失效
    艾美球虫（Eimeria-Membran），更多的是免疫原蛋白。

    公开(公告)号：EP0382531A1

    公开(公告)日：1990-08-16

    申请号：EP90301340.7

    申请日：1990-02-08

    申请人： Merck & Co., Inc.

    发明人： Gurnett, Anne M. ,  Turner, Mervyn J. ,  St John Crane, Mark.

    IPC分类号： A61K39/012 ,  C07K15/04 ,  C07K3/00

    CPC分类号： C07K14/455 ,  A61K39/00

    摘要： Novel Eimeria membrane associated proteins with immunogenic properties are identified, purified and characterized. The cellular location of the membrane associated proteins is characterized by their partitioning properties in the detergent Triton X114 and by the presence on a subset of the proteins of a hydrophobic glycolipid anchor. Antiserum raised against detergent phase proteins reacts specifically with the surface of Eimeria sporozoites. The Eimeria proteins are capable of protecting poultry from coccidiosis.

    摘要翻译： 鉴定，纯化和表征具有免疫原性的新颖艾美球虫膜相关蛋白。 膜相关蛋白的细胞位置的特征在于它们在洗涤剂Triton X114中的分配性质以及在疏水性糖脂锚的蛋白质子集上的存在。 针对洗涤剂相蛋白产生的抗血清特异性地与艾美球虫子孢子的表面反应。 艾美球虫蛋白能够保护家禽免于球虫病。

    Coccidiosis vaccine
    77.
    发明公开
    Coccidiosis vaccine 失效
    球虫病，Impfstoff。

    公开(公告)号：EP0256536A2

    公开(公告)日：1988-02-24

    申请号：EP87111820.4

    申请日：1987-08-14

    申请人： Chilwalner Partnership

    发明人： Wallach, Michael ,  Pugatsch, Thea ,  Mencher, David

    IPC分类号： A61K39/012 ,  C12N1/10 ,  C07K14/455 ,  C07K1/00 ,  C12P21/00 ,  G01N33/48 ,  A61K39/395 ,  C07H21/00 ,  C12N15/00

    CPC分类号： C07K16/20 ,  A61K39/00 ,  A61K39/012 ,  C07K14/455 ,  F02B2075/027

    摘要： Substantially purified Eimeria maxima gametocytes have been prepared and methods for their preparation are disclosed. A proteinaceous extract derived from the gametocytes comprises at least nine proteins of vary­ing molecular weights. Vaccines for conferring upon a chicken active immunity against infection comprise an effective immunizing amount of Eimeria maxima gameto­cytes or gametocyte extract and a carrier. Also pro­vided is a method if in vitro development of Eimeria spp. gametocytes or oocysts and a method of assaying drug or anti-gametocyte antibody effectivity on Eimeria spp. development in vitro .

    摘要翻译： 已经制备了大量纯化的艾美球虫配子体，并公开了其制备方法。 衍生自配子细胞的蛋白质提取物包含至少9种不同分子量的蛋白质。 赋予鸡免受感染感染的疫苗包括有效免疫​​量的艾美球虫最大配子体配子体或配子体提取物和载体。 还提供了一种如果艾美球虫体外发育的方法。 配子体或卵囊，以及测定药物或抗配偶体抗体对艾美球虫属的有效性的方法。 体外发育。

    Antigenic proteins and vaccines containing them and protective antibodies directed to them for prevention of coccidiosis caused by Eimeria tenella and Eimeria necatrix
    78.
    发明公开
    Antigenic proteins and vaccines containing them and protective antibodies directed to them for prevention of coccidiosis caused by Eimeria tenella and Eimeria necatrix 失效
    抗生素蛋白质和包含它们的疫苗和保护性抗体，用于预防艾米利亚·泰尼拉和艾美豹NECATRIX引起的预防结核病

    公开(公告)号：EP0164176A3

    公开(公告)日：1987-05-27

    申请号：EP85200889

    申请日：1985-06-05

    申请人： SOLVAY & Cie (Société Anonyme)

    发明人： Newman, Jr., Karel Z. ,  Tedesco, John L. ,  Simonson, Randy R. ,  Gore, Thomas C. ,  Petersen, Gary R. ,  Brothers, Virginia Mary ,  Files, James Gordon ,  Paul, Leland Shawn

    IPC分类号： A61K39/012 ,  C12N15/00 ,  C12P21/00 ,  A61K39/395

    CPC分类号： C07K16/425 ,  A61K38/00 ,  A61K39/00 ,  C07K14/455 ,  C07K16/20

    摘要： A purified antigenic protein has been obtained which is capable of inducing in a chicken an immune response conferring protection against infection by Eimeria tenella or Eimeria necatrix. The protein has a molecular weight of about 25,000 and is composed of two polypeptides joined by a disulfide bond. The two polypeptide subunits have molecular weights of about 17,000 and about 8,000, respectively. The gene encoding the protein has been sequenced and the amino acid sequence of the protein deduced therefrom and by direct peptide sequencing. The protein and antigenic polypeptides having an amino acid sequence included within the protein may be incorporated into a vaccine for conferring upon a chicken active immunity against infection by E. tenella and E. necatrix. A hybridoma cell line (ATCC No. HB8561) has been developed which produces a monoclonal antibody designated Ptn 7.2A4/4. This antibody may be used to confer upon a chicken specific passive protection against infection by E. tenella and E. necatrix. The antibody may also be used to obtain the purified protein antigen and the 11,500 and 6,500 dalton polypeptide fragments thereof. Finally, an anti-idiotype antibody to the Ptn. 7.2A4/4 monoclonal antibody may be prepared and used to confer upon a chicken active immunity against E. tenella and E. necatrix infection. Legend * Initial amino acid of 17.000 dalton peptide ** Pinal amino acid of 17.000 dalton peptide + Initial amino acid of 8,000 dalton peptide ++ Pinal amino acid of 8,000 dalton peptide Key to ambiguous bases 3= Probably C 5 Probably A 7 Maybe C 8 Maybe T 0 Maybe C Y C or T M = C or G

    Antigenic proteins and vaccines containing them and protective antibodies directed to them for prevention of coccidiosis caused by Eimeria tenella
    80.
    发明公开
    Antigenic proteins and vaccines containing them and protective antibodies directed to them for prevention of coccidiosis caused by Eimeria tenella 失效
    含有对他们的相同疫苗，保护性抗体用于预防由禽艾美球虫球虫病的抗原蛋白。

    公开(公告)号：EP0164176A2

    公开(公告)日：1985-12-11

    申请号：EP85200889.5

    申请日：1985-06-05

    申请人： SOLVAY

    发明人： Newman, Jr., Karel Z. ,  Tedesco, John L. ,  Simonson, Randy R. ,  Gore, Thomas C. ,  Petersen, Gary R. ,  Brothers, Virginia Mary ,  Files, James Gordon ,  Paul, Leland Shawn

    IPC分类号： A61K39/012 ,  C12N15/00 ,  C07K13/00

    CPC分类号： C07K16/425 ,  A61K38/00 ,  A61K39/00 ,  C07K14/455 ,  C07K16/20

    摘要： A purified antigenic protein has been obtained which is capable of inducing in a chicken an immune response conferring protection against infection by Eimeria tenella or Eimeria necatrix. The protein has a molecular weight of about 25,000 and is composed of two polypeptides joined by a disulfide bond. The two polypeptide subunits have molecular weights of about 17,000 and about 8,000, respectively. The gene encoding the protein has been sequenced and the amino acid sequence of the protein deduced therefrom and by direct peptide sequencing.
    The protein and antigenic polypeptides having an amino acid sequence included within the protein may be incorporated into a vaccine for conferring upon a chicken active immunity against infection by E. tenella and E. necatrix.
    A hybridoma cell line (ATCC No. HB8561) has been developed which produces a monoclonal antibody designated Ptn 7.2A4/4. This antibody may be used to confer upon a chicken specific passive protection against infection by E. tenella and E. necatrix. The antibody may also be used to obtain the purified protein antigen and the 11,500 and 6,500 dalton polypeptide fragments thereof.
    Finally, an anti-idiotype antibody to the Ptn. 7.2A4/4 monoclonal antibody may be prepared and used to confer upon a chicken active immunity against E. tenella and E. necatrix infection. Legend * Initial amino acid of 17.000 dalton peptide ** Pinal amino acid of 17.000 dalton peptide + Initial amino acid of 8,000 dalton peptide ++ Pinal amino acid of 8,000 dalton peptide Key to ambiguous bases 3= Probably C 5 Probably A 7 Maybe C 8 Maybe T 0 Maybe C Y C or T M = C or G

    摘要翻译： 纯化的抗原蛋白已被所有的，其能够在免疫应答由禽艾美球虫或毒害艾美耳球虫赋予针对感染的保护作用在鸡诱导而获得的。 所述蛋白具有约25,000的分子量，并且是由通过二硫键连接的两个多肽。 两个多肽亚基具有约17000至约8000的分子量，分别。 编码该蛋白质的基因已被测序，并从与通过直接肽测序推导存在蛋白质的氨基酸序列。 该蛋白质和具有包含在蛋白质内的氨基酸序列抗原多肽可被并入的疫苗，用于在针对感染的鸡主动免疫由禽艾美球虫和E.球虫赋予。 的杂交瘤细胞系（ATCC号HB8561）已经被开发，其产生指定PTN 7.2A4 / 4的单克隆抗体。 这种抗体可被用于在针对感染的由禽艾美球虫和E.球虫鸡特定被动保护赋予。 因此，该抗体可用于获得纯化的蛋白质抗原及其11500个6500道尔顿的多肽片段。 最后，抗独特型抗体对PTN。 7.2A4 / 4单克隆抗体可以被制备并用于在针对禽艾美球虫和E.球虫感染鸡主动免疫来赋予。