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公开(公告)号：EP3286594A2
公开(公告)日：2018-02-28
申请号：EP16718898
申请日：2016-04-25
发明人： KAPANIDIS ACHILLEFS , JING BO
CPC分类号： G02B21/24 , G02B21/16 , G02B21/361 , G02B21/362
摘要： A compact microscope comprising an enclosure,a support element, a primary optical support element located within the enclosure and supported by the support element, at least one vibration isolating mount between the support element and the primary optical support element, a sample stage supported on the primary optical support element to support a sample, a return optical system to receive returned light from a sample and transmit returned light to a detection apparatus, wherein the return optical system is mounted on the primary optical support element, and wherein the compact microscope comprises at least one of the following elements; a) an objective lens system, the objective lens system being supported on the primary optical support element, an illumination section and an illumination optical system to direct an illumination light beam from the illumination section to the sample stage, and a mirror disposed above the sample stage, the illumination optical system being arranged to direct light through the objective lens system to the mirror; b) a temperature-control system, the temperature control system comprising a temperature control circuit comprising a plurality of fluid-carrying channels within at least one of the enclosure and the primary optical support element; and c) the return optical system being operable to separate returned light into at least a first wavelength band and a second wavelength band, and the detection apparatus comprising an imaging apparatus, the return optical system having a first tube lens to focus returned light in a first wavelength band to a first area of the imaging apparatus and a second tube lens to focus returned light in a second wavelength band to a second area of the imaging apparatus.
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公开(公告)号：EP2581778A4
公开(公告)日：2017-11-22
申请号：EP11792583
申请日：2011-06-13
申请人： NIPPON KOGAKU KK
发明人： SASE ICHIRO
CPC分类号： G02B21/16 , G01N21/6458 , G02B21/06 , G02B21/367 , G02B27/56
摘要： A microscope apparatus includes: a distribution measurement apparatus which - with respect to an observation region wherein a sample is arranged which comprises a fluorescent material that is activated when irradiated with an activating light of a prescribed wavelength, and that emits fluorescence when irradiated in an activated condition with an exciting light of a wavelength that differs from that of the activating light - obtains a fluorescent picture image by conducting irradiation with the exciting light, and measures a fluorescent intensity distribution of the observation region; an irradiation intensity setting apparatus which sets irradiation intensities of the activating light for respective portions of the observation region based on the fluorescent intensity distribution; and a picture image formation apparatus which obtains a plurality of the fluorescent picture images by multiply repeating operations comprising an operation wherein the observation region is irradiated with the activating light at the irradiation intensities that have been set in the respective portions, and an operation wherein a fluorescent picture image is obtained by irradiating the observation region with the exciting light after the activating light irradiation, and which generates a sample picture image from the plurality of the fluorescent picture images.
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公开(公告)号：EP2034349B1
公开(公告)日：2017-10-25
申请号：EP07767085.9
申请日：2007-06-08
申请人： Nikon Corporation
发明人： MAIYA, Nobuhiko
CPC分类号： G02B21/367 , C12M41/14 , C12M41/46 , G02B21/0088 , G02B21/16
摘要： An observing apparatus includes: a photographic unit that takes a region where cells are present as an observation object region based upon a macro image captured at low magnification of an interior of a cell culture vessel, and photographs the cells in the observation object region; an illuminating unit that irradiates light upon the cells only during photography by the photographic unit; and an output unit that outputs a fluorescent image photographed by the photographic unit while irradiating light only during photography by the illuminating unit.
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74.发明公开ILLUMINATION FILTER FOR AN ILLUMINATION FILTER SYSTEM OF A SURGICAL MULTISPECTRAL FLUORESCENCE MICROSCOPE HAVING A SPATIAL FILTER PATTERN 审中-公开具有空间滤波器模式的外科多光谱荧光显微照明系统的照明滤光片
公开(公告)号：EP3205257A1
公开(公告)日：2017-08-16
申请号：EP16155624.6
申请日：2016-02-15
发明人： Themelis, George , Kuster, Manfred
CPC分类号： G01N21/6458 , A61B1/043 , A61B1/0646 , F21V9/40 , G01N2021/6471 , G01N2201/068 , G02B5/201 , G02B21/06 , G02B21/16 , G02B26/008 , G02B26/023
摘要： The invention relates to an illumination filter (36, 44) for an illumination filter system (2) for medical imaging, in particular multispectral fluorescence imaging, as performed e.g. in a microscope (1) or endoscope, in particular a multispectral fluorescence microscope. The present invention provides an illumination filter for medical imaging, in particular a multispectral fluorescence imaging, that is capable of capturing simultaneously more than one fluorescence signal, and allow a homogeneous illumination for obtaining different images from the object illuminated by comprising a spatial filter pattern (43) masking a defined filtering fraction of a first illumination path (47) on the filter and masking a defined filtering fraction of a second illumination path (48, 49, 50) on the filter, wherein the filtering fraction of the first and the second illumination paths (47, 48, 49, 50) are different. The invention further relates to an illumination filter system (2) for medical imaging, in particular multispectral fluorescence imaging, as performed e.g. in a microscope (1) or endoscope, in particular a multispectral fluorescence microscope, comprising such illumination filter (36, 44)
摘要翻译： 本发明涉及一种用于医疗成像，尤其是多光谱荧光成像的照明滤波器系统（2）的照明滤波器（36,44），例如， 在显微镜（1）或内窥镜中，特别是多光谱荧光显微镜中。 本发明提供了一种用于医学成像，特别是多光谱荧光成像的照明滤波器，其能够同时捕获多于一个荧光信号，并且允许均匀照明以通过包括空间滤波器模式（ 43）遮蔽所述滤光片上的第一照明路径（47）的限定的滤光片段并遮蔽所述滤光片上的第二照明路径（48,49,50）的限定的滤光片段，其中所述第一和第二 照明路径（47,48,49,50）是不同的。 本发明进一步涉及用于医疗成像，特别是多光谱荧光成像的照明滤波器系统（2），例如， 在显微镜（1）或内窥镜中，特别是多光谱荧光显微镜中，包括这种照明滤波器（36,44）
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75.发明公开APERTURE SCANNING FOURIER PTYCHOGRAPHIC IMAGING 审中-公开BLENDENABTASTENDE PTYCHOGRAFISCHE FOURIER-BILDGEBUNG
公开(公告)号：EP3028088A4
公开(公告)日：2017-03-15
申请号：EP14832857
申请日：2014-07-31
发明人： HORSTMEYER ROARKE , ZHENG GUOAN , OU XIAOZE , YANG CHANGHUEI
IPC分类号： G02B26/10 , G01N23/205 , G01T1/185 , G01T3/00 , G02B21/00 , G02B21/16 , G02B21/36 , H01J37/26
CPC分类号： G02B21/008 , G01N23/205 , G01T1/185 , G01T3/008 , G02B21/0032 , G02B21/0048 , G02B21/0056 , G02B21/006 , G02B21/16 , G02B21/367 , G02B27/46 , G02B27/58 , H04N13/0459
摘要： Certain aspects pertain to aperture-scanning Fourier ptychographic imaging devices comprising an aperture scanner that can generate an aperture at different locations at an intermediate plane of an optical arrangement, and a detector that can acquire lower resolution intensity images for different aperture locations, and wherein a higher resolution complex image may be constructed by iteratively updating regions in Fourier space with the acquired lower resolution images.
摘要翻译： 某些方面涉及光圈扫描傅里叶成像设备，其包括能够在光学布置的中间平面处的不同位置处生成光圈的光圈扫描仪以及能够针对不同光圈位置获取较低分辨率强度图像的检测器，并且其中a 通过用获取的较低分辨率图像迭代地更新傅立叶空间中的区域，可以构建较高分辨率的复合图像。
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公开(公告)号：EP2325684B1
公开(公告)日：2017-02-01
申请号：EP09812947.1
申请日：2009-06-10
CPC分类号： G02B21/0088 , G01N21/6458 , G02B21/0076 , G02B21/16 , G02B21/26 , G02B21/34
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77.发明授权
公开(公告)号：EP2453241B1
公开(公告)日：2017-01-11
申请号：EP11181564.3
申请日：2006-05-23
CPC分类号： G01N21/6428 , G01N21/64 , G01N21/6458 , G01N21/648 , G01N33/582 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G02B21/16 , G02B21/367 , G02B27/58
摘要： The invention relates to a method comprising steps of: providing spatially-structured activation radiation having relatively high- and relatively low-intensity regions to a sample that includes phototransformable optical labels ("PTOLs") to activate a subset of the PTOLs in the sample located predominately at relatively high intensity regions of the spatially-structured activation radiation; providing spatially-structured excitation radiation to the subset of activated PTOLs in the sample, wherein the exciting radiation is structured so that one or more relatively high intensity regions of the excitation radiation at least partially overlap one or more relatively high intensity regions of the activating radiation; detecting radiation emitted from the activated and excited PTOLs with imaging optics; and controlling the intensities and spatial structures of the activating radiation and the exciting radiation so that radiation emitted from PTOLs in the sample is emitted substantially from at least one volume that is comparable to or less than a diffraction-limited resolution volume ("DLRV") of the imaging optics. The PTOLs may be formed by photoactivated or photoswitched fluorescent proteins. The invention also relates to a corresponding system.
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公开(公告)号：EP3108281A1
公开(公告)日：2016-12-28
申请号：EP15706749.7
申请日：2015-02-19
CPC分类号： G02B21/008 , G02B21/0032 , G02B21/0036 , G02B21/006 , G02B21/0076 , G02B21/16 , G02B21/365 , G02B27/0075 , H04N3/02 , H04N5/372
摘要： The invention relates to a method for light sheet microscopy. The arrangement comprises: - means for scanning a sample volume (10) to be imaged with a light sheet (9), which encloses an angle δ ≠ 90° with the optical axis (8) of an objective lens (1), wherein - the light sheet (9) penetrates in the propergation direction the entire sample volume (10) to be imaged, and wherein - the depth of focus S
obj of the objective lens (1) is smaller than the depth T of said sample volume (10) in the direction of the optical axis (8), - an optical device downstream of the objective, designed to increase the depth of focus S
obj to a depth of focus S
eff , which is greater than or equal to the depth T of said sample volume (10), - means for positioning the sample volume (10) within the region of the depth of focus S
eff , - a spatial opto-electronic surface sensor (17) downstream of the optical device, and hardware and software, designed for generating images of the sample volume (10) from the electronic image signals emitted from the surface sensor (17).摘要翻译： 一种用于光板显微镜的装置，其包括用光板扫描样品体积的装置，其包括与物镜的光轴成δ°≠90°的角度。 光片在传播方向上通过整个样本体积，并且物镜的景深Sobj小于该样本体积的光轴深度T. 设置在物镜下游的光学装置将场景Sobj的景深增加到该样本体积的深度S eff≥深度T. 该装置还包括用于将样本体积定位在景深Seff的区域内的装置。 空间分辨的光电区域传感器设置在光学装置的下游，并且提供硬件和软件以从由区域传感器输出的电子图像信号产生样本体积图像。
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公开(公告)号：EP2997353A4
公开(公告)日：2016-12-28
申请号：EP14797836
申请日：2014-05-15
IPC分类号： G01N21/64 , A61B5/00 , A61B10/02 , A61B90/00 , G01N1/30 , G02B21/06 , G02B21/16 , G02B21/36 , G06K9/52 , G06K9/62 , H04N5/225
CPC分类号： G02B21/16 , A61B5/0071 , A61B10/02 , A61B2090/3941 , G01N1/30 , G01N21/6458 , G01N2001/302 , G01N2021/6419 , G01N2021/6421 , G01N2021/6441 , G01N2201/0635 , G01N2201/0675 , G01N2201/129 , G02B21/0076 , G02B21/365 , G02B21/367 , G06K9/52 , G06K9/6267 , H04N5/2256
摘要： Systems and methods are provided for evaluating a fresh tissue sample, prepared as to fluoresce under illumination, during a medical procedure. A structured light source is configured to project a spatially patterned light beam onto the fresh tissue sample. An imaging system is configured to produce an image from fluorescence emitted from the illuminated fresh tissue sample. A system control is configured to provide a human-comprehensible clinically useful output associated with the medical procedure.
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公开(公告)号：EP2453240B1
公开(公告)日：2016-12-28
申请号：EP11181563.5
申请日：2006-05-23
CPC分类号： G01N21/6428 , G01N21/64 , G01N21/6458 , G01N21/648 , G01N33/582 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G02B21/16 , G02B21/367 , G02B27/58
摘要： The invention relates to a method comprising steps of: providing spatially-structured activation radiation having relatively high- and relatively low-intensity regions to a sample that includes phototransformable optical labels ("PTOLs") to activate a subset of the PTOLs in the sample located predominately at relatively high intensity regions of the spatially-structured activation radiation; providing spatially-structured excitation radiation to the subset of activated PTOLs in the sample, wherein the exciting radiation is structured so that one or more relatively high intensity regions of the excitation radiation at least partially overlap one or more relatively high intensity regions of the activating radiation; detecting radiation emitted from the activated and excited PTOLs with imaging optics; and controlling the intensities and spatial structures of the activating radiation and the exciting radiation so that radiation emitted from PTOLs in the sample is emitted substantially from at least one volume that is comparable to or less than a diffraction-limited resolution volume ("DLRV") of the imaging optics. The PTOLs may be formed by photoactivated or photoswitched fluorescent proteins. The invention also relates to a corresponding system.
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