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264 条记录 (耗时 0.023 秒)

    VARIANT LUCIFERASE, VARIANT LUCIFERASE GENE, NOVEL RECOMBINANT DNA AND PROCESS FOR PRODUCING VARIANT LUCIFERASE
    81.
    发明公开
    VARIANT LUCIFERASE, VARIANT LUCIFERASE GENE, NOVEL RECOMBINANT DNA AND PROCESS FOR PRODUCING VARIANT LUCIFERASE 有权
    萤光素酶变体中，荧光素酶变体中，重组DNA和过程的GEN用于生产荧光素酶VARIANT

    公开(公告)号：EP1148139A1

    公开(公告)日：2001-10-24

    申请号：EP99959952.5

    申请日：1999-12-21

    申请人： KIKKOMAN CORPORATION

    发明人： KAJIYAMA, Naoki ,  NAKANO, Eiichi

    IPC分类号： C12N15/53 ,  C12N9/02 ,  C12N1/21

    CPC分类号： C12N9/0069 ,  C12N9/0004

    摘要： The present invention relates to a variant luciferase having improved luminescence sustention when compared with a wild type luciferase; a variant luciferase gene encoding the amino acid sequence of this luciferase; a recombinant DNA characterized in that it is constructed by insertion of this variant luciferase gene into a vector DNA; a transformant or a transductant comprising this recombinant DNA; and a method for producing a variant luciferase, which comprises culturing the above transformant or transductant in a medium and collecting the variant luciferase from the culture product.

    摘要翻译： 本发明涉及一种变体萤光素酶具有改善的发光苏斯坦灰当与野生型荧光素酶相比; 编码该荧光素酶的氨基酸序列的变体萤光素酶基因; 为特征的DASS它的重组DNA是通过此变体萤光素酶基因插入载体DNA的插入构建; 的转化体或转导体一个包含该重组DNA; 和用于产生变体萤光素酶，其包括在培养基中培养上述转化体或转导体，并收集从培养产物变体萤光素酶的方法。

    CREATINE AMIDINOHYDROLASE AND PROCESS FOR PRODUCING THE SAME
    83.
    发明公开
    CREATINE AMIDINOHYDROLASE AND PROCESS FOR PRODUCING THE SAME 审中-公开
    KRERTIN-AMIDOHYDROLASE UND VERFAHREN ZU DEREN HERSTELLUNG

    公开(公告)号：EP1134284A1

    公开(公告)日：2001-09-19

    申请号：EP99972676.3

    申请日：1999-11-25

    申请人： KIKKOMAN CORPORATION

    发明人： FURUKAWA, Keisuke ,  ICHIKAWA, Toshio

    IPC分类号： C12N9/78

    CPC分类号： C12N9/78 ,  C12Y305/03003

    摘要： The present invention relates to a creatine amidinohydrolase having the following physicochemical properties: (a) hydrolyzing 1 mole of creatine to generate 1 mole of sarcosine and 1 mole of urea, (b) having a substrate specificity to creatine, (c) having an optimum pH ranging from 6.0 to 7.0, particularly a pH of about 6.5, (d) having a stable pH ranging from 4.0 to 11.0, (e) having an optimum temperature ranging from 50 to 55°C, and (f) having a molecular weight of approximately 92,000 daltons (as measured by gel filtration); and to a method for producing a creatine amidinohydrolase, which comprises, culturing a microorganism having an ability to produce the creatine amidinohydrolase, and recovering the creatine amidinohydrolase from the obtained culture. The creatine amidinohydrolase of the invention is characterized by being insusceptible to bilirubin when measuring creatinine, since its optimum pH is in the weakly acidic range.

    摘要翻译： 本发明涉及具有以下物理化学性质的肌酸脒基水解酶：（a）水解1摩尔肌酸以产生1摩尔肌氨酸和1摩尔尿素，（b）对肌酸具有底物特异性，（c）具有最佳 pH范围为6.0至7.0，特别是约6.5的pH值，（d）pH值范围为4.0至11.0，（e）最适温度为50至55℃，（f）具有分子量 约92,000道尔顿（通过凝胶过滤测量）; 以及制备肌酸脒基水解酶的方法，其包括培养具有产生肌酸脒基水解酶的能力的微生物，并从所得培养物中回收肌酸脒基水解酶。 本发明的肌酸酰氨基水解酶的特征在于测量肌酸酐时不易胆红素，因为其最佳pH处于弱酸性范围。

    A multiply transformed Aspergillus (koji) mold and a method of manufacturing a flavor enhancer using the same
    84.
    发明公开
    A multiply transformed Aspergillus (koji) mold and a method of manufacturing a flavor enhancer using the same 审中-公开
    一个经常转化的黑（曲）真菌及其用于生产风味增强剂使用

    公开(公告)号：EP1132461A1

    公开(公告)日：2001-09-12

    申请号：EP01302132.4

    申请日：2001-03-08

    申请人： KIKKOMAN CORPORATION

    发明人： Umitsuki, Genryou, c/o Kikkoman Corporation ,  Sato, Hiroe, c/o Kikkoman Corporation ,  Sugishita, Misao, Kikkoman Corporation ,  Fukushima, Misao, c/o Kikkoman Corporation ,  Koyama, Yasuji, c/o Kikkoman Corporation

    IPC分类号： C12N1/14 ,  C12N9/62 ,  A23J3/34 ,  C12P21/06

    CPC分类号： C12N1/14 ,  A23J3/346 ,  A23L27/50 ,  C12N9/62

    摘要： The present invention provides a koji mold having increased protease activity and peptidase activity relative to a parent strain, a method of breeding the koji mold, and a method of manufacturing a flavor enhancer using the koji mold. More specifically, the present invention provides:

    (1) a koji mold having increased protease activity and peptidase activity relative to a parent strain obtained by transformation using a protease gene and a peptidase gene.
    (2) a method of breeding the above koji mold which comprises transforming a parent strain of koji mold using a protease gene and a peptidase gene, and then selecting a transformant having higher protease activity and peptidase activity relative to a parent strain.
    (3) a method of manufacturing a flavor enhancer
    which comprises allowing a culture product of the above koji mold to act on a protein.

    摘要翻译： 本发明提供了具有增加的蛋白酶活性和活性相肽酶的亲本菌株，育种霉曲菌的方法，以及使用该曲菌制造风味增强剂的方法的酒曲霉菌。 更具体地，本发明提供：（1）具有增加的蛋白酶活性和活性肽相对通过使用蛋白酶基因和肽酶基因转化而得到的亲本菌株曲霉。 （2）饲养上述酒曲霉菌，其包括使用蛋白酶基因和肽酶基因转化曲霉的亲本株，然后选择具有更高的蛋白酶活性和活性相肽酶的亲本株的转化体的方法。 （3）一种制造风味增强剂包括使上面的霉曲菌的培养产物作用于蛋白质的方法。

    Method and kit for testing microbial drug sensitivity, and method and kit for measuring minimum inhibitory concentration for microorganisms
    86.
    发明公开
    Method and kit for testing microbial drug sensitivity, and method and kit for measuring minimum inhibitory concentration for microorganisms 失效
    一种微生物的药物的敏感性测试，方法和试剂盒用于Missung微生物的最小抑制浓度的方法和试剂盒

    公开(公告)号：EP0844309A1

    公开(公告)日：1998-05-27

    申请号：EP97309137.4

    申请日：1997-11-13

    申请人： KIKKOMAN CORPORATION

    发明人： Hattori, Noriaki ,  Nakajima, Moto-o ,  Yajitate, Keiko

    IPC分类号： C12Q1/02

    CPC分类号： C12Q1/18 ,  Y10S435/975

    摘要： There are provided a method and a kit for testing microbial drug resistance and a method and a kit for measuring minimum inhibitory concentration for a microorganism, in which highly reliable evaluation results are obtained by preventing false evaluation based on false-resistance of microorganisms to a drug. The method of testing microbial drug sensitivity comprises inoculating and culturing a microorganism in a drug-containing medium to obtain a medium containing the microorganism morphologically transformed by the influence of the drug, adding an ATP elution agent and an ATP deletion agent to the culture to delete the eluted ATP and other ATP in the medium, and comparing the amount of ATP in the microorganism remaining in the culture with the amount of ATP in the microorganism separately cultured in a medium not containing said drug and treated in the same manner as above. The method of measuring minimum inhibitory concentration for a microorganism is carried out in the same manner as above.

    摘要翻译： 本发明提供一种方法以及用于测试微生物耐药性和一种方法以及用于测量最小抑制浓度为微生物的试剂盒，其中，高度可靠的评价结果​​通过基于微生物的假电阻对药物防止误评价得到的试剂盒 ， 测试微生物药物敏感性的方法包括接种，并在含有药物的培养基中培养微生物，得到含有由所述药物的影响形态学转化的微生物的培养基中，加入到ATP洗脱剂和ATP缺失剂培养物以删除 洗脱的ATP和其它ATP在介质中，和ATP的量残留在培养物与ATP的微生物中在不包含所述药物并以相同的方式处理的培养基中培养的分开量的微生物相比较。 用于测量的微生物的最小抑制浓度的方法，以相同的方式如上述进行。