公开(公告)号：EP3037171A1

公开(公告)日：2016-06-29

申请号：EP15199104.9

申请日：2015-12-10

申请人： Miltenyi Biotec GmbH

发明人： KAULING, M. Burgund ,  HUPPERT, Volker ,  DOSE, Christian ,  STEINBRÜCK, Philipp

IPC分类号： B03C1/00 ,  C12N5/00 ,  G01N33/543 ,  B03C1/01 ,  B03C1/28

CPC分类号： C12N13/00 ,  B03C1/01 ,  B03C1/288 ,  B03C2201/26 ,  C12N5/0087 ,  G01N1/40 ,  G01N33/54333 ,  G01N2001/4038

摘要： The invention is directed to a method for enriching target cells from a sample of cells characterized by:
a) contacting the sample with a cell aggregation agent and first magnetic particles having an iron content of 0.1 pg to 5000 pg, coupled to a first antigen recognizing moiety; and second magnetic particles having an iron content of 0.05 fg to 100 fg and coupled to a second antigen recognizing moiety to obtain mixture a)
b) applying a first magnetic field gradient to the mixture a) thereby removing the cells bound to the first antigen recognizing moiety coupled to the first magnetic particles, to obtain a mixture b) and obtaining an agglomerate comprising the cells of mixture a) bound to the cell aggregation agent
c) applying a second magnetic field gradient to the mixture b) thereby immobilizing the cells bound to the second antigen recognizing moiety coupled to the second magnetic particles
d) recovering the immobilized cells from the second magnetic field gradient as target cells.

摘要翻译： 本发明涉及用于从细胞样品中富集靶细胞的方法，其特征在于：a）使样品与细胞聚集剂和铁含量为0.1pg至5000pg的第一磁性颗粒接触，第一磁性颗粒与第一抗原识别 部分; 和铁含量为0.05fg至100fg并与第二抗原识别部分偶联以获得混合物的第二磁性颗粒a）b）向混合物施加第一磁场梯度a）从而除去结合到第一抗原识别的细胞 部分偶联至第一磁性颗粒，以获得混合物b）并获得包含与细胞聚集剂结合的混合物a）的细胞的附聚物c）向混合物施加第二磁场梯度b）从而将结合到 耦合到第二磁性颗粒的第二抗原识别部分d）从作为靶细胞的第二磁场梯度回收固定化细胞。

公开(公告)号：EP3026060A1

公开(公告)日：2016-06-01

申请号：EP14194847.1

申请日：2014-11-26

申请人： Miltenyi Biotec GmbH

发明人： Thie, Holger ,  Tomiuk, Stefan ,  Bürger, Iris ,  Nölle, Volker

IPC分类号： C07K16/28

CPC分类号： C07K16/32 ,  C07K16/289 ,  C07K2317/24 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/567 ,  C07K2317/92

摘要： The present invention provides a humanized antibody or fragment thereof specific for the antigen CD45R0, wherein said antibody or fragment thereof comprises a humanized heavy chain variable domain comprising a CDR1 region of SEQ ID NO:1, a CDR2 region of SEQ ID NO:2, and a CDR3 region of SEQ ID NO:3, and a humanized light chain variable domain comprising a CDR1 region of SEQ ID NO:4, a CDR2 region of SEQ ID NO:5, and a CDR3 region of SEQ ID NO:6. It is also provided the use of the present antibody or fragment thereof for enrichment or depletion of CD45R0-expressing cells from a sample comprising CD45R0-expressing cells.

摘要翻译： 本发明提供对抗原CD45R0特异性的人源化抗体或其片段，其中所述抗体或其片段包含人源化重链可变结构域，其包含SEQ ID NO：1的CDR1区，SEQ ID NO：2的CDR2区， 和SEQ ID NO：3的CDR3区，以及包含SEQ ID NO：4的CDR1区，SEQ ID NO：5的CDR2区和SEQ ID NO：6的CDR3区的人源化轻链可变结构域。 还提供了本抗体或其片段用于从包含CD45R0表达细胞的样品中富集或消耗表达CD45R0的细胞的用途。

公开(公告)号：EP2993227A3

公开(公告)日：2016-06-01

申请号：EP15180326.9

申请日：2015-08-10

申请人： Miltenyi Biotec GmbH ,  Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. -Hans-Knöll-Institut -

发明人： SCHEFFOLD, Alexander ,  BACHER, Petra ,  KNIEMEYER, Olaf ,  TEUTSCHBEIN, Janka ,  BRAKHAGE, Axel

IPC分类号： C12N5/0783 ,  A61K39/00 ,  G01N33/50 ,  G01N33/569

CPC分类号： A61K39/0002 ,  A61K2039/57 ,  G01N33/505 ,  G01N2333/38 ,  G01N2333/705

摘要： The present invention provides the in-vitro use of at least one in-vivo-target antigen of Aspergillus fumigatus for selective activation, detection and/or analysis of Aspergillus fumigatus specific CD4 + T cells in a sample comprising cells, wherein said at least one in-vivo-target antigen reveals an immune reactivity characterized by i) the in vivo existence of antigen-specific T cells comprising more than 60% memory T cells and ii) said antigen-specific T cells further comprise T cells able to produce IFN-gamma upon stimulation at a frequency between 15% and 80% and/or IL17 upon stimulation at a frequency between 5% and 30%. Said at least one in-vivo-target antigen may be selected from the group consisting of antigens Scw4, Pst1, Shm2, GliT and TpiA or fragments thereof. Also provided are a method, a composition, and a kit thereof.

摘要翻译： 本发明提供了体外使用烟曲霉的至少一种体内靶抗原用于在包含细胞的样品中选择性激活，检测和/或分析烟曲霉特异性CD4 + T细胞，其中所述至少一种 活体靶抗原显示免疫反应性，其特征在于i）体内存在包含超过60％记忆T细胞的抗原特异性T细胞，和ii）所述抗原特异性T细胞还包含能够产生IFN-γ的T细胞 在以介于5％和30％之间的频率进行刺激时以15％至80％的频率刺激和/或刺激IL17。 所述至少一种体内靶抗原可以选自抗原Scw4，Pst1，Shm2，GliT和TpiA或其片段。 还提供了其方法，组合物和试剂盒。

公开(公告)号：EP2993227A2

公开(公告)日：2016-03-09

申请号：EP15180326.9

申请日：2015-08-10

申请人： Miltenyi Biotec GmbH ,  Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. -Hans-Knöll-Institut -

发明人： SCHEFFOLD, Alexander ,  BACHER, Petra ,  KNIEMEYER, Olaf ,  TEUTSCHBEIN, Janka ,  BRAKHAGE, Axel

IPC分类号： C12N5/0783 ,  A61K39/00 ,  G01N33/50 ,  G01N33/569

CPC分类号： A61K39/0002 ,  A61K2039/57 ,  G01N33/505 ,  G01N2333/38 ,  G01N2333/705

摘要： The present invention provides the in-vitro use of at least one in-vivo-target antigen of Aspergillus fumigatus for selective activation, detection and/or analysis of Aspergillus fumigatus specific CD4 + T cells in a sample comprising cells, wherein said at least one in-vivo-target antigen reveals an immune reactivity characterized by i) the in vivo existence of antigen-specific T cells comprising more than 60% memory T cells and ii) said antigen-specific T cells further comprise T cells able to produce IFN-gamma upon stimulation at a frequency between 15% and 80% and/or IL17 upon stimulation at a frequency between 5% and 30%. Said at least one in-vivo-target antigen may be selected from the group consisting of antigens Scw4, Pst1, Shm2, GliT and TpiA or fragments thereof. Also provided are a method, a composition, and a kit thereof.

摘要翻译： 本发明提供体外使用烟曲霉的至少一个体内靶抗原用于在包含细胞的样品中选择性激活，检测和/或分析烟曲霉特异性CD4 + T细胞，其中所述至少一种 体内靶抗原揭示了免疫反应性，其特征在于i）包含超过60％记忆T细胞的抗原特异性T细胞的体内存在，和ii）所述抗原特异性T细胞还包含能够产生IFN- 在以5％至30％之间的频率刺激时以15％至80％之间的频率和/或IL17刺激时的γ。 所述至少一种体内靶抗原可以选自抗原Scw4，Pst1，Shm2，GliT和TpiA或其片段。 还提供了一种方法，组合物和试剂盒。

公开(公告)号：EP2808037A1

公开(公告)日：2014-12-03

申请号：EP13169416.8

申请日：2013-05-28

申请人： Miltenyi Biotec GmbH

发明人： Dose, Christian, Dr. ,  Eckardt, Dominik, Dr. ,  Steiner, Mark-Steven, Dr.

IPC分类号： A61K49/18

CPC分类号： A61K49/1863 ,  A61K49/1896

摘要： The invention relates to paramagnetic beads comprising iron oxide and aminodextran having a zeta potential of 5 to 100 mV.
The paramagnetic beads can be used for in vivo cell tracking by MRI.

摘要翻译： 本发明涉及顺磁珠，其包括铁氧化物和氨基葡聚糖具有5至100mV的ζ电势。 所述顺磁性珠粒可用于通过MRI体内细胞跟踪。

公开(公告)号：EP2781523A1

公开(公告)日：2014-09-24

申请号：EP13159766.8

申请日：2013-03-18

申请人： Miltenyi Biotec GmbH

发明人： Uhlmann, Eugen, Dr. ,  Smicius, Dr., Romualdas ,  Rockel, Dr., Thomas

IPC分类号： C07H21/04 ,  A61K31/7088 ,  A61P31/12 ,  A61P37/00 ,  A61P35/00

CPC分类号： C12N15/113 ,  C07H21/04 ,  C12N2310/11 ,  C12N2310/351 ,  G01N33/5011

摘要： Lipophilic oligonucleotide comprising a phosphate glycerol unit containing at least one aliphatic unsaturated carbon bond according to formula I

with
Oligonucleotide =
an unmodified or modified nucleic acid of 2 - 1000 nucleotides in length
R =
a bond or a linker unit
Y =
OH, SH or NHR3
X and Z =
independently O, S or NR3
R3 =
hydrogen or branched or unbranched and/or substituted or unsubstituted alkyl, aryl and/or alkyl aryl residue with 10 to 30 carbon atoms
R1, R2 =
branched or unbranched and/or substituted or unsubstituted alkyl, aryl and/or alkylaryl residue with 10 to 30 carbon atoms, with the provisio that at least one of the residues R1 or R2 comprises at least one aliphatic carbon-carbon double bond
Use of lipophilic oligonucleotide according to Formula I for drug discovery or for transfection of cells.

摘要翻译： 包含含有至少一个根据式I的脂族不饱和碳键的磷酸甘油单元的亲脂性寡核苷酸与寡核苷酸=长度为2至1000个核苷酸的未修饰或修饰的核酸R =一个键或连接基单元Y = OH，SH或NHR3 X和Z独立地为O，S或NR 3 R 3 =氢或支链或非支链和/或取代或未取代的具有10至30个碳原子的烷基，芳基和/或烷基芳基R 1，R 2 =支链或非支链和/或取代或 未取代的具有10至30个碳原子的烷基，芳基和/或烷芳基残基，条件是残基R 1或R 2中的至少一个包含至少一个脂族碳 - 碳双键使用根据式I的亲脂寡核苷酸用于药物发现 或用于转染细胞。

公开(公告)号：EP2700713A1

公开(公告)日：2014-02-26

申请号：EP12181138.4

申请日：2012-08-21

申请人： Miltenyi Biotec GmbH

发明人： Schüler, Anne ,  Eckardt, Nicole Susann, Dr. ,  Bürger, Iris

IPC分类号： C12N15/79 ,  C12N15/10

CPC分类号： C12N15/65 ,  C12N15/52 ,  C12N2830/20 ,  C12N2840/203 ,  C12P21/00 ,  C12P21/02 ,  C12Q1/6897 ,  C12Q2563/107 ,  C12Q2565/626

摘要： The present invention provides a polynucleotide and a method for screening and enriching a recombinant protein expressed in a eukaryotic cell, the polynucleotide comprising a tricistronic expression cassette comprising a) a promoter, b) a gene of interest (GOI), c) a reporter gene, d) a selection marker gene, e) an internal ribosome entry site (IRES) element, and f) a 2A element. Said method comprises a) transfecting or transducing suitable eukaryotic host cells with the polynucleotides of the present invention, b) culturing the eukaryotic host cells under conditions so as to express the protein of interest, the reporter protein and the selection marker protein in a cell culture selection medium suitable for selecting positively-transfected/transduced cells by means of said selection marker, and c) screening, sorting and/or enriching the cells expressing high levels of protein of interest by means of the reporter protein.

摘要翻译： 本发明提供的多核苷酸和用于筛选和富集在真核细胞中过表达重组蛋白，所述多核苷酸包含一个三顺反子表达盒，其包含a）启动子，b）中的感兴趣的基因（GOI），c）中的报告基因的方法 ，D）的选择标记基因，E）内部核糖体进入位点（IRES）元件的元件，和f）2A。 所述方法包括：a）转染或转导合适的真核宿主细胞与本发明的多核苷酸，b）培养所述真核宿主细胞的条件下，以表达目的在细胞培养物的蛋白，报告蛋白和选择标记蛋白 选择培养基适于选择正转染/转导的细胞由所述选择标记物的手段;和c）筛选，分选和/或富集的细胞中，通过报道蛋白的手段表达高水平感兴趣的蛋白质的。

公开(公告)号：EP2540394A1

公开(公告)日：2013-01-02

申请号：EP11171503.3

申请日：2011-06-27

申请人： Miltenyi Biotec GmbH

发明人： Peters, Ralf-Peter Dr. ,  Kabaha, Eiad Dr. ,  Stöters, Wolfgang ,  Winkelmayer, Gerhard ,  Bucher, Franz G.

IPC分类号： B01L3/00 ,  B01F7/16 ,  B01F13/10 ,  B01F15/02 ,  B02C19/22 ,  G01N1/28

CPC分类号： B01L3/502 ,  B01F7/1695 ,  B01F13/1044 ,  B01F15/00844 ,  B01F2215/0431 ,  B01F2215/0477 ,  B01F2215/0481 ,  B01L3/50825 ,  B01L2200/026 ,  B01L2300/046 ,  B01L2300/0681 ,  B02C19/0056 ,  B02C19/22 ,  G01N1/286 ,  G01N2001/2873

摘要： The invention is directed to a device for fragmenting or homogenizing biological samples, comprising:
- a tubular laboratory test vessel (1) having an open first end (3) and a second end closed by a bottom (10);
- a closing lid (2) for the laboratory test vessel (1) connected to the open end (3) of the laboratory test vessel (1);
- a processing tool (5) arranged in said closing lid (2), comprising a plurality of teeth (4a-c) and an agitating element (11) having a guidance tube (13) that is rotatably mounted through a central bore in the lid (2) and includes an engagement portion (16) that is adapted to be engaged by a drive motor; wherein at least one outer cutting blade (6) is located at the periphery of the processing tool (5) and a transportation element (8) is arranged at the guidance tube (13) that extends outwardly in a helical shape over the outer cutting blade (6) and which is provided with slots (6a) fitting the outer cutting blades (6).
Furthermore, the invention is directed to a process for extracting, fragmenting, mixing or homogenizing biological samples in a device according to any of the claims 1 to 12 wherein the agitating element (11) is rotated relative to the closing lid (2) and the processing tool (5), thereby guiding the transportation element (8) over at least one group of teeth (4 a-c) and at least one outer cutting blade (6) through slots (6a) of the transportation element (8) in a grinding and cutting manner.

摘要翻译： 本发明涉及用于使生物样品分裂或均化的装置，包括： - 管状实验室测试容器（1），其具有敞开的第一端（3）和由底部（10）封闭的第二端; - 连接到实验室测试容器（1）的开口端（3）的用于实验室测试容器（1）的关闭盖（2）; - 布置在所述关闭盖（2）中的处理工具（5），包括多个齿（4a-c）和搅拌元件（11），所述搅拌元件具有引导管（13），所述引导管通过所述导向管 盖（2），并包括适于与驱动马达接合的接合部分（16）; 其中至少一个外切割刀片（6）位于所述加工工具（5）的周边处，并且在所述引导管（13）处布置有输送元件（8），所述引导管（13）以螺旋形状向外延伸到所述外切割刀片 （6），并且设置有装配外切割刀片（6）的槽（6a）。 此外，本发明涉及一种用于在根据权利要求1至12中任一项所述的装置中提取，分段，混合或均化生物样品的方法，其中所述搅拌元件（11）相对于所述关闭盖（2）旋转，并且 处理工具（5），从而通过运送元件（8）的槽（6a）在磨削中将运输元件（8）引导至少一组齿（4交流）和至少一个外切割刀片（6） 切割方式。

公开(公告)号：EP2240082A1

公开(公告)日：2010-10-20

申请号：EP09702031.7

申请日：2009-01-19

申请人： Miltenyi Biotec GmbH

发明人： SCHREINER, Thomas

IPC分类号： A61B10/02 ,  A61B10/00 ,  A61M1/36

CPC分类号： A61B10/025 ,  A61B10/0096 ,  A61B2010/0258 ,  A61M1/0218

摘要： The invention relates to a device (1) for removing biological material from an organism. The device (1) comprises: a removal means (2) for removing biological material from an organism, having a bypass means (3) for the selective lumen connection of the removal means (2) to a) a penetration means (6) for penetration into the organism, b) a receiving means (4) for receiving the material from the organism, and c) a storage means (12) for storing a substance. The bypass means (3) is designed such that the removal means (2) can be connected in a lumen manner to the storage means (12) and the receiving means (4), or to the penetration means (6).

公开(公告)号：EP2227334A2

公开(公告)日：2010-09-15

申请号：EP08858277.0

申请日：2008-12-08

申请人： Miltenyi Biotec GmbH

发明人： MILTENYI, Stefan ,  SCHIMMELPFENNIG, Winfried ,  LANTOW, Holger ,  NEUSCHÄFER, Elmar, Niklas ,  BIEHL, Martin ,  KABAHA, Eiad ,  SCHULZ, Jürgen

IPC分类号： B04B5/04 ,  B04B13/00 ,  G01N15/04

CPC分类号： G01N33/58 ,  A61M1/0209 ,  A61M1/3618 ,  A61M1/3633 ,  A61M1/3679 ,  A61M1/3692 ,  A61M1/3693 ,  A61M1/3696 ,  A61M2202/0071 ,  A61M2202/0413 ,  A61M2202/0427 ,  A61M2202/10 ,  B01D15/3809 ,  B01D15/3885 ,  B01D21/26 ,  B03C1/002 ,  B03C1/027 ,  B03C1/032 ,  B03C1/0332 ,  B03C1/0335 ,  B03C1/034 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  B04B1/12 ,  B04B5/00 ,  B04B5/04 ,  B04B5/0442 ,  B04B5/10 ,  B04B7/08 ,  B04B13/00 ,  B04B2005/0464 ,  B04B2013/006 ,  C12M23/28 ,  C12M33/10 ,  C12M41/48 ,  C12M47/04 ,  C12N5/0636 ,  C12N5/0646 ,  C12N5/0647 ,  C12N5/0669 ,  G01N35/0098 ,  G01N2333/70596

摘要： The invention relates to a system, comprising: a) a sample processing unit, comprising an input port and an output port coupled to a rotating container having at least one sample chamber, the sample processing unit configured provide a first processing step to a sample or to rotate the container so as to apply a centrifugal force to a sample deposited in the chamber and separate at least a first component and a second component of the deposited sample; and b) a sample separation unit coupled to the output port of the sample processing unit, the cell separation unit comprising separation column holder, a pump and a plurality of valves configured to at least partially control fluid flow through a fluid circuitry and a separation column positioned in the holder, the separation column configured to separate labeled and unlabeled components of sample flowed through the column.