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    Biocatalytic oxidation using legume, rice and malvaceous plant peroxidases, preparing phenolic resins using such oxidation, and method for purifying peroxidase enzymes
    81.
    发明公开
    Biocatalytic oxidation using legume, rice and malvaceous plant peroxidases, preparing phenolic resins using such oxidation, and method for purifying peroxidase enzymes 失效
    利用蔬菜，大米和Malvegewächsen过氧化物酶生物催化氧化; 酚醛树脂和用于纯化的过氧化物酶处理的制剂。

    公开(公告)号：EP0481815A2

    公开(公告)日：1992-04-22

    申请号：EP91309652.5

    申请日：1991-10-18

    申请人： THE MEAD CORPORATION

    发明人： Johnson, Mark A. ,  Cyrus, William L., Jr. ,  Pokora, Alexander R.

    IPC分类号： C12P7/22 ,  C08G61/00 ,  C12N9/02

    CPC分类号： C12N9/0065 ,  C08G61/00 ,  C12P7/22

    摘要： Biocatalytic oxidative processes are described whrein oxidizable substrates are reacted with peroxides in rthe presence of a peroxidase such as soybean peroxidase, another legume peroxidase or peroxidase derived from rice or a malvaceous plant such as cotton. Oxidative coupling reactions for producing phenolic resins using such peroxidases are also described. A method for the purification of peroxidase enzyme-containing extracts, generally, is also described.

    摘要翻译： 生物催化氧化过程中描述whrein可氧化基底与过氧化物反应。在过氧化物酶的存在下rthe：如大豆过氧化物酶，另一种豆科植物过氧化物酶或过氧化物酶来自稻或malvaceous计划衍生：例如棉花。 用于使用搜索过氧化物酶酚醛树脂的氧化偶合反应是这样描述的。 一种用于过氧化物酶的含酶提取物，基因反弹的纯化方法中，如此描述。

    Mitochondrial P450
    82.
    发明公开
    Mitochondrial P450 失效
    线粒体P450。

    公开(公告)号：EP0477961A2

    公开(公告)日：1992-04-01

    申请号：EP91116485.3

    申请日：1991-09-26

    申请人： SUMITOMO CHEMICAL COMPANY, LIMITED

    发明人： Akiyoshi, Megumi ,  Yabusaki, Yoshiyasu ,  Sakaki, Toshiyuki ,  Ohkawa, Hideo

    IPC分类号： C12N15/53 ,  C12N15/62 ,  C12N9/02 ,  C12N15/81 ,  C12N1/19 ,  C12P7/22

    CPC分类号： C12N9/0077 ,  C07K2319/00 ,  C07K2319/02 ,  C07K2319/07 ,  C12N9/0085 ,  C12N15/625 ,  C12N15/81 ,  C12P7/02 ,  C12P33/00 ,  C12Y114/99009

    摘要： The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome.
    An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5β-cholestane-3α, 7α, 12α, 27-tetrol, 25-hydroxyvitamin D₃ and 1α,25-dihydroxyvitamin D₃ are also disclosed.

    摘要翻译： 公开了线粒体P450在酵母中的表达。 线粒体P450包括嵌合P450，其中存在于哺乳动物线粒体P450的N末端的信号序列已被酵母线粒体蛋白的信号和嵌合P450取代，其中后一信号被靶向进一步取代 信号序列到微粒体。 用于大规模生产酶的表达质粒，携带表达质粒的重组酵母菌株，使用重组酵母生产酶的方法，以及生产5β-胆甾烷-3α，7α ，12α，27-四醇，25-羟基维生素D3和1α，25-二羟基维生素D3。

    Biochemical process
    84.
    发明公开
    Biochemical process 失效
    生化过程

    公开(公告)号：EP0252567A3

    公开(公告)日：1989-02-08

    申请号：EP87201286.9

    申请日：1987-07-07

    申请人： SHELL INTERNATIONALE RESEARCH MAATSCHAPPIJ B.V.

    发明人： Schofield, John Antony

    IPC分类号： C12P7/02 ,  C12P7/22

    CPC分类号： C12R1/40 ,  C12P7/02 ,  C12P7/22 ,  G01N33/567 ,  Y10S435/877

    摘要： A process for the preparation of a catechol and/or a compound comprising a 1,2-dihydroxycyclohexa-3,5-diene ring, which comprises growing a microorganism in the presence of a carbon source, the microorganism being capable of converting the corresponding aromatic compound into the catechol and/or the compound comprising a 1,2-dihydroxycyclohexa-3,5-diene ring; and supplying the corresponding aromatic compound to the microorganism; characterized in that the carbon source is molasses.

    Developer sheets, recording materials and resins useful therein.
    86.
    发明公开
    Developer sheets, recording materials and resins useful therein. 失效
    显影片，记录材料和benützbare树脂。

    公开(公告)号：EP0245365A4

    公开(公告)日：1987-11-25

    申请号：EP86906671

    申请日：1986-10-24

    申请人： MEAD CORP

    发明人： POKORA ALEXANDER R ,  CYRUS WILLIAM L JR

    IPC分类号： B41M5/132 ,  B41M5/155 ,  C08G61/00 ,  C08G61/02 ,  C08G61/10 ,  C08G65/38 ,  C08G65/44 ,  C12P7/22 ,  C12P7/62 ,  C12P13/00

    CPC分类号： B41M5/155 ,  C08G61/10

    摘要： In a process for the preparation of a phenolic resin, a phenol is reacted with a peroxidase or an oxidase enzyme and a peroxide in an organic solvent containing medium to generate phenolic radicals which react to form phenolic resins and, more particularly, formaldehyde-free resins of formula (I), where n is an integer greater than or equal to 2, the phenolic units of the resin are directly bonded to one another through positions ortho or para to the hydroxyl group, Y is present at a position meta or para to the hydroxy group and is selected from the group consisting of an alkyl group, a halogen atom, an aryl group, a phenylalkyl group, an allyl group, a carboxyl group of the formula -COOR, where R is a hydrogen atom, an alkyl group or a phenylalkyl group, an amino group of the formula -NR1R2 where R1 and R2 are the same or different and represent a hydrogen atom or an alkyl group, and Z is a hydrogen atom, an alkyl group, a halogen atom, an aryl group, a phenylalkyl group, or a -COOR group, or Z in conjunction with the adjacent meta position forms a condensed benzene ring; or a metal-modified, formaldehyde-free phenolic developer resin obtained by reacting said developer resin with a metal salt; the resins are useful as developers in forming colored images by reaction with substantially colorless electron-donating compounds.

    Method and materials for the microbiological oxidation of aromatic hydrocarbons
    88.
    发明公开
    Method and materials for the microbiological oxidation of aromatic hydrocarbons 失效
    Verfahren und Materialien zur mirkobiologischen Oxydation von aromatischen Kohlewasserstoffen。

    公开(公告)号：EP0103887A2

    公开(公告)日：1984-03-28

    申请号：EP83109332.3

    申请日：1983-09-20

    申请人： AMGEN INC.

    发明人： Ensley, Burt D.

    IPC分类号： C12N15/00 ,  C12P17/02 ,  C12P7/22 ,  C12P7/24 ,  C12P7/42

    CPC分类号： C12P7/24 ,  C12N15/52 ,  C12P7/00 ,  C12P7/22 ,  C12P7/42

    摘要： Disclosed are novel DNA sequences susceptible to expression in a microorganism in the form of synthesis of one or more enzymes participative in the microbiological oxidative degradation of a selected aromatic hydrocarbon substrate to a selected oxidized compound which is an intermediate in a multiple enzyme-catalyzed degradative pathway for total mineralization of said substrate. The DNA sequences are characterized by being substantially free from operative association with DNA sequences susceptible to expression in the form of synthesis of one or more enzymes participative in oxidative degradation of said selected intermediate compound. Illustrative of the invention is a plasmidborne DNA sequence of Pseudomonas putida origin which codes for expression, in a host microorganism such as E coli, of enzymes participative in the oxidative degradation of naphthalene to salicylate. Cultured growth of stably transformed host cells in a medium including a naphthalene substrate results in quantitative naphthalene degradation and accumulation of isolatable quantities of, e.g., salicylate products.

    摘要翻译： 公开了以微生物表达的新型DNA序列，其以合成一种或多种参与选择的芳族烃底物的微生物氧化降解为选择的氧化化合物（其是多酶催化的降解途径中的中间体）的酶的形式 用于所述基底的总矿化。 DNA序列的特征在于基本上没有与以参与所述选择的中间体化合物的氧化降解的一种或多种酶的合成形式表达的DNA序列的有效结合。 本发明的实例是恶臭假单胞菌来源的质粒载体DNA序列，其编码在宿主微生物如大肠杆菌中表达参与萘至水杨酸的氧化降解的酶。 在包含萘底物的培养基中培养稳定转化的宿主细胞的生长导致定量的萘降解和可分离量的例如水杨酸盐产物的积累。

    EDIBLE PLANT CELL CULTURE BIOMASS BASED ON FRUIT ENDOCARP AND/OR SEED EXPLANTS AND METHODS OF ITS PREPARATION
    89.
    发明公开
    EDIBLE PLANT CELL CULTURE BIOMASS BASED ON FRUIT ENDOCARP AND/OR SEED EXPLANTS AND METHODS OF ITS PREPARATION 审中-公开

    公开(公告)号：EP4461129A1

    公开(公告)日：2024-11-13

    申请号：EP23172320.6

    申请日：2023-05-09

    申请人： ZHAW - Zürcher Hochschule für Angewandte Wissenschaften

    发明人： EIBL, Regine ,  EIBL, Dieter ,  HÜHN, Tilo ,  MOZAFFARI, Fruhar

    IPC分类号： A01H4/00 ,  C12P7/22 ,  A01H5/08 ,  A01H6/52

    摘要： A method of processing plant cell cultures based on fruit explants is described, comprising the steps of: a) inducing callus from an explant of a fruit selected from drupes, nuts, legumes or berries on callus induction medium and propagating the callus on callus growth medium; b) selecting a callus clone from the grown callus culture and initiating a suspension cell culture comprising dedifferentiated cells by inoculating the selected callus clone into a suspension cell culture medium; c) growing fruit suspension cells for a time and under conditions sufficient to produce a suspension including a biomass comprising secondary metabolites; and d) separating the biomass from the suspension to provide a liquid phase and a solid, in vitro-manufactured biomass and optionally extracting secondary metabolites from the in vitro -manufactured biomass to provide a secondary metabolite extract, wherein the fruit is selected from and the explant contains endocarp and/or seed material from the drupes, nuts, legumes or berries. The method enables biomass to be manufactured in vitro at improved efficiencies and yields. In addition, in vitro-manufactured biomass prepared by the aforementioned method, uses of the same, and related food compositions are described.