SYNTHETIC LONG READ DNA SEQUENCING
    9.
    发明公开
    SYNTHETIC LONG READ DNA SEQUENCING 有权
    SYNTHETISCHE LONG-READ-DNA-SEQUENZIERUNG

    公开(公告)号:EP3055431A4

    公开(公告)日:2017-05-10

    申请号:EP14852570

    申请日:2014-09-24

    申请人: STC UNM

    IPC分类号: C12Q1/68

    摘要: The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter polynucleotide reads. Generally, The method includes annealing a plurality of primers to a denatured DNA molecule, appending a barcode polynucleotide to the 5′ end of the primer, subjecting the DNA molecules to a plurality of cycles of (1) pooling, (2) dividing, and (3) appending a barcode polynucleotide to the 5′ end of the primer, sequencing the barcode polynucleotides and the genomic DNA, and assembling the short read polynucleotide sequences having identical barcode polynucleotides.

    摘要翻译: 本公开描述了通过组装多个较短的多核苷酸读数对DNA序列的长部分进行测序的方法。 通常,该方法包括将多个引物退火至变性的DNA分子,将条形码多核苷酸附加至引物的5'末端,使DNA分子经历多次(1)合并,(2)分裂和 (3)将条形码多核苷酸附加到引物的5'末端,对条形码多核苷酸和基因组DNA进行测序,并组装具有相同条形码多核苷酸的短读取多核苷酸序列。