DNA AMPLIFICATION METHOD
    10.
    发明公开
    DNA AMPLIFICATION METHOD 审中-公开
    DNA-AMPLIFIKATIONSVERFAHREN

    公开(公告)号:EP1752535A1

    公开(公告)日:2007-02-14

    申请号:EP05743478.9

    申请日:2005-05-30

    CPC classification number: C12Q1/686 C12Q2549/119

    Abstract: The object of the invention is to provide a DNA amplification method with which single-strand DNA of a desired direction and a desired region can be prepared simply and efficiently. The invention provides a DNA amplification method that uses DNA as a template, wherein the DNA amplification method uses a first primer that is complementary to the 3' end side of a target base sequence, a second primer that has a base sequence that is homologous to the 5' end side of that target base sequence, and a third primer that has, on its 3' end side, a base sequence that is homologous to part of the base sequence on the 5' end side of the second primer or the first primer, and a melting temperature Tm 1 of the first primer, a melting temperature Tm 2 of the second primer, and a melting temperature Tm 3 of the third primer have the relationship expressed by the following expression: Tm 1 Tm 2 and Tm 2 Tm 3 and
    wherein the DNA amplification method includes a first process of performing a PCR cycle at an annealing temperature Ta (where Ta≤Tm 1 and Ta≤Tm 2 ), and a second process of performing a PCR cycle at an annealing temperature Tb (where Tm 1 2 3 ).

    Abstract translation: 本发明的目的是提供一种可以简单且有效地制备所需方向和所需区域的单链DNA的DNA扩增方法。 本发明提供了使用DNA作为模板的DNA扩增方法,其中DNA扩增方法使用与靶碱基序列的3'端侧互补的第一引物,具有与 该目标碱基序列的5'端侧和第三引物,其3'末端具有与第二引物的5'末端侧的碱基序列的部分同源的碱基序列或第一引物 引物,第一引物的熔解温度Tm 1,第二引物的熔解温度Tm 2和第三引物的熔解温度Tm 3具有由以下表达式表示的关系:Tm 1

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