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公开(公告)号：EP2598648A1
公开(公告)日：2013-06-05
申请号：EP11741110.8
申请日：2011-07-27
申请人： Universite de Rouen , Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) , Algenics
发明人： BARDOR, Muriel , LOUVET, Romain , SAINT-JEAN, Bruno , BUREL, Carole , BAIET, Bérangère , CADORET, Jean-Paul , LEROUGE, Patrice , MICHEL, Rémy , CARLIER, Aude
IPC分类号： C12P21/00 , C12N9/10 , C12N9/24 , C12N1/13 , C07K14/505
CPC分类号： C12N15/8243 , C07K14/505 , C12N9/1051 , C12N9/2402 , C12P21/005 , C12Y302/01096 , Y02P20/52
摘要： The present invention concerns transformed Phaeodactylum tricomutum comprising a nucleic acid sequence operatively linked to a promoter, wherein said nucleic acid sequence encodes an N-acetylglucosaminyltransferase I and/or an a-Mannosidase II and wherein at least one β-Ν-acetylglucosaminidase of said transformed Phaeodactylum tricomutum has been inactivated; a method for producing a glycosylated polypeptide, said method comprising the steps of (i) culturing a transformed P. tricomutum as defined previously and (ii) purifying said polypeptide that is expressed and glycosylated in said transformed P. tricomutum; and the use of such a transformed P. tricomutum for producing a glycosylated polypeptide.
摘要翻译： 本发明涉及包含可操作地连接到启动子的核酸序列的转化的三叶黄瓜，其中所述核酸序列编码N-乙酰葡糖胺基转移酶I和/或± - 甘露糖苷酶II，并且其中至少一个所述转化的2-N-乙酰氨基葡糖苷酶 三角褐指藻已灭活; 一种制备糖基化多肽的方法，所述方法包括以下步骤：（i）培养如前所定义的转化的三角褐豆胡桃，和（ii）纯化在所述转化的三角褐豆中表达和糖基化的所述多肽; 以及使用这种经转化的三角褐指藻来生产糖基化多肽。
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公开(公告)号：EP2598648B1
公开(公告)日：2017-11-08
申请号：EP11741110.8
申请日：2011-07-27
申请人： Université de Rouen , Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER) , Algenics
发明人： BARDOR, Muriel , LOUVET, Romain , SAINT-JEAN, Bruno , BUREL, Carole , BAIET, Bérangère , CADORET, Jean-Paul , LEROUGE, Patrice , MICHEL, Rémy , CARLIER, Aude
IPC分类号： C12P21/00 , C12N9/10 , C12N9/24 , C12N1/13 , C07K14/505
CPC分类号： C12N15/8243 , C07K14/505 , C12N9/1051 , C12N9/2402 , C12P21/005 , C12Y302/01096 , Y02P20/52
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公开(公告)号：EP2155880A1
公开(公告)日：2010-02-24
申请号：EP08772802.8
申请日：2008-06-13
发明人： D'AOUST, Marc-Andre , MARQUET-BLOUIN, Estelle , BARDOR, Muriel , BUREL, Carole , FAYE, Loic , LEROUGE, Patrice , VEZINA, Louis-Philippe , GOMORD, Véronique , AQUIN, Stéphanie , RIHOUEY, Christophe , PACCALET, Thomas , SOURROUILLE, Christophe
IPC分类号： C12N15/62 , A01H5/00 , A01H5/10 , C07K19/00 , C12N15/54 , C12N15/82 , C12N5/10 , C12N9/10 , C12P21/02 , C12P21/08
CPC分类号： C12N15/8257 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8258
摘要： A method for synthesizing a protein of interest with a modified N-glycosylation profile within a plant, a portion of a plant, or a plant cell is provided. The method comprises co-expressing within a plant a nucleotide sequence encoding a first nucleotide sequence encoding a hybrid protein (GNT1-GalT) comprising a CTS domain of N-acetylglucosaminyl transferase (GNT1) fused to a catalytic domain of beta-1,4galactosyltransferase (GalT), the first nucleotide sequence operatively linked with a first regulatory region that is active in the plant, and a second nucleotide sequence for encoding the protein of interest, the second nucleotide sequence operatively linked with a second regulatory region that is active in the plant. The first and second nucleotide sequences are co-expressed to synthesize a protein of interest comprising glycans with the modified N-glycosylation profile within the plant, the portion of the plant, or the plant cell.
摘要翻译： 提供了在植物，植物的一部分或植物细胞内合成具有修饰的N-糖基化分布的目的蛋白质的方法。 所述方法包括在植物内共表达编码编码包含N-乙酰葡糖胺基转移酶（GNT1）的CTS结构域的杂合蛋白（GNT1-GalT）的第一核苷酸序列的核苷酸序列，所述CTS结构域与β-1,4半乳糖基转移酶的催化结构域 GalT），第一核苷酸序列与在植物中有活性的第一调控区有效连接，第二核苷酸序列用于编码感兴趣的蛋白质，第二核苷酸序列与在植物中有活性的第二调控区有效连接 。 第一和第二核苷酸序列共表达以合成包含植物内植物，植物部分或植物细胞内具有修饰的N-糖基化谱的聚糖的目的蛋白质。
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4.发明公开PRODUCTION OF GLYCOSYLATED POLYPEPTIDES IN MICROALGAE 审中-公开米格兰根的HERSTELLUNG GLYKOSYLERERTER POLYPEPTIDE
公开(公告)号：EP2257618A1
公开(公告)日：2010-12-08
申请号：EP09710039.0
申请日：2009-02-12
申请人： Institut Francais de Recherche pour l'Exploitation de la Mer (IFREMER) , Centre National de la Recherche Scientifique - CNRS , UNIVERSITE DE ROUEN
发明人： CADORET, Jean-Paul , CARLIER, Aude , LEROUGE, Patrice , BARDOR, Muriel , BUREL, Carole , MAURY, Florian
CPC分类号： C12P21/005 , C07K14/4756 , C07K14/505 , C12N1/12 , C12N9/1051 , C12N9/2402 , C12N15/79 , C12N15/8257 , C12Y302/01045 , C12Y302/01096 , C12Y305/01052
摘要： The invention relates to transformed microalgae capable of expressing glycosylated polypeptides and methods for producing said transformed microalgae and producing glycosylated polypeptides.
摘要翻译： 本发明涉及能够表达糖基化多肽的转化微藻和用于产生所述转化的微藻并产生糖基化多肽的方法。
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公开(公告)号：EP1987137B1
公开(公告)日：2011-06-22
申请号：EP07710612.8
申请日：2007-02-09
发明人： PACCALET, Thomas , BARDOR, Muriel , RIHOUEY, Christophe , GOMORD, Véronique , FAYE, Loïc , LEROUGE, Patrice , AQUIN, Stéphanie , VEZINA, Louis-Philippe , D'AOUST, Marc-André
IPC分类号： C12N15/82 , C12N15/54 , C12N15/60 , C12N5/04 , C12N5/10 , C12N9/88 , C12N9/90 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8245 , C12N9/1085 , C12N9/88 , C12N15/8257 , C12P19/26 , C12P21/005 , C12Y205/01057 , C12Y401/03003
摘要： A method of synthesizing sialic acid in plants, and plants capable of synthesizing sialic acid is provided. Furthermore, a method of producing sialylated protein in a plant is also provided. The method to synthesize sialic acid comprises providing a plant comprising a nucleotide sequence encoding N-acetyl neuraminic acid (Neu5Ac) synthase or Neu5Ac lyase, and expressing the nucleotide sequence thereby synthesizing sialic acid. The plant may also co-express a nucleotide sequence encoding one or more than one of an epimerase, a CMP-Neu5Ac synthase, a CMP-Neu5Ac transporter and a sialyltransferase.
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公开(公告)号：EP4269432A1
公开(公告)日：2023-11-01
申请号：EP22305618.5
申请日：2022-04-26
摘要： Monoclonal antibodies represent the most rapidly growing category of the recombinant therapeutic protein pipeline, with more than 85% of the therapeutic indications. The present invention proposes novel vectors that have been optimized so as to produce high levels of monoclonal antibodies when transfected in the microalgae Phaeodactylum tricornutum, as well as optimized culture conditions of said microalgae cells. Altogether, the present invention provides new systems for producing high amounts of monoclonal antibodies, functional fragments or derivatives thereof, in microalgae.
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公开(公告)号：EP2155880B1
公开(公告)日：2016-08-10
申请号：EP08772802.8
申请日：2008-06-13
发明人： D'AOUST, Marc-Andre , MARQUET-BLOUIN, Estelle , BARDOR, Muriel , BUREL, Carole , FAYE, Loic , LEROUGE, Patrice , VEZINA, Louis-Philippe , GOMORD, Véronique , AQUIN, Stéphanie , RIHOUEY, Christophe , PACCALET, Thomas , SOURROUILLE, Christophe
IPC分类号： C12N15/62 , A01H5/00 , A01H5/10 , C07K19/00 , C12N15/54 , C12N15/82 , C12N5/10 , C12N9/10 , C12P21/02 , C12P21/08
CPC分类号： C12N15/8257 , C12N9/1051 , C12N15/8216 , C12N15/8218 , C12N15/8245 , C12N15/8258
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