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公开(公告)号：EP4144837A1
公开(公告)日：2023-03-08
申请号：EP20933800.3
申请日：2020-05-29
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MOU, Huiyan , CAO, Shan
摘要： Provided are a transaminase mutant and an application thereof. Compared with an amino acid sequence shown in SEQ ID NO:1, an amino acid sequence of the transaminase mutant includes at least one of the following mutation sites: L166, K149, K146, A168, H73, F133, H82, E24, V194, T294, A295, G235 and F236. The mutant of the present invention has the improved catalytic activity for a transammonization reaction of ketone substrates, and is suitable for industrial production of chiral amines.
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公开(公告)号：EP4276188A1
公开(公告)日：2023-11-15
申请号：EP21924111.4
申请日：2021-07-06
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MOU, Huiyan , WANG, Zujian , SUN, Kaihua , JIA, Ru , LIU, Fang , LIU, Wenjing
摘要： Provided is a method for synthesizing a chiral amine compound. A transaminase is used to transaminate a ketone substrate under the action of an amino donor, to obtain the chiral amine compound; and the conserved amino acid sequence region of the transaminase at least includes a region 1 (MAGLWCVN) and a region 2 (YNTFFKT). With the transaminase with the specific conserved amino acid sequence region to synthesize a large sterically hindered chiral amine, the enzyme catalytic reaction volume is small, the synthesizing route is short, the product yield is high, a high-cost noble metal is not required for catalysis under the synthesizing conditions, three wastes are reduced, and the production cost is saved.
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公开(公告)号：EP3536780A1
公开(公告)日：2019-09-11
申请号：EP16920642.2
申请日：2016-11-04
申请人： Asymchem Laboratories (Tianjin) Co., Ltd. , Asymchem Life Science (Tianjin) Co., Ltd. , Tianjin Asymchem Pharmaceuticals Co., Ltd. , Asymchem Laboratories (Fuxin) Co., Ltd. , Jilin Asymchem Laboratories Co., Ltd.
发明人： HONG, Hao , JAMES, Gage , LU, Jiangping , ZHANG, Na , YU, Wenyan , LIU, Fang , LI, Yanjun , HUANG, Xin , GAO, Juan , ZHANG, Kejian , MA, Yulei , WEI, Junlu
摘要： Provided are a proline hydroxylase and uses thereof. The proline hydroxylase comprises (a) a protein having the amino acid sequence as shown in SEQ ID NO: 2; (b) a protein having an amino acid sequence of SEQ HD NO: 2 with a mutation of one or more amino acids and having a proline hydroxylase activity; or(c) a protein retaining the mutation of one or more amino acids as in (b), and having the proline hydroxylase activity and having at least 78% homology with the amino acid sequence of the protein in (b). Protein having the amino acid sequence as shown in SEQ HD NO: 2 and mutants obtained by genetically engineering have higher catalytic specificity or significantly increased catalytic activity when compared to proline hydroxylases in prior art.
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公开(公告)号：EP4273237A1
公开(公告)日：2023-11-08
申请号：EP21924110.6
申请日：2021-07-06
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , ZHANG, Na , JIAO, Xuecheng , MA, Yulei , MOU, Huiyan , WANG, Zujian , SUN, Kaihua , LI, Xiang , ZHAO, Tong , CAO, Shan
摘要： Provided are transaminase mutants and uses thereof. The transaminase mutant is obtained by one or more amino acid mutations occurring in SEQ ID NO: 2 or is a mutant with a conserved amino acid mutation obtained by taking the sequence SEQ ID NO: 1 of a wild-type CvTA transaminase as a reference. Compared with wild-type transaminases, the catalytic activity of the mutant is improved to different degrees, so that the production efficiency of chiral amine compound synthesis may be improved.
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公开(公告)号：EP4053269A1
公开(公告)日：2022-09-07
申请号：EP19951177.5
申请日：2019-10-28
发明人： HONG, Hao , JAMES, Gage , XIAO, Yi , MA, Yulei , ZHANG, Na , JIAO, Xuecheng , MU, Huiyan , CHENG, Yibing , CAO, Shan
摘要： Provided are a transaminase mutant and use thereof. The transaminase mutant has an amino acid sequence obtained by mutation of an amino acid sequence shown in SEQ ID NO:1, the mutation at least includes one of the following mutation site combinations: T7C+S47C, Q78C+A330C, V137C+G313C, A217C+Y252C and L295C+C328C; or the transaminase mutant has an amino acid sequence which has the mutation sites in the mutated amino acid sequence and has 80% or more identity with the mutated amino acid sequence. The transaminase mutant realizes the change of protein structure and functions, reduces the enzyme amount, increases the enantiomeric excess (ee) value of a product, and reduces the difficulty of post-processing, so that the transaminase mutant may be suitable for industrial production.
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