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公开(公告)号：EP2598172A1
公开(公告)日：2013-06-05
申请号：EP11741727.9
申请日：2011-07-29
发明人： SIEKMANN, Juergen , HAIDER, Stefan , ROTTENSTEINER, Hanspeter , IVENS, Andreas , TURECEK, Peter , ZOECHLING, Oliver
IPC分类号： A61K47/48
摘要： The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
摘要翻译： 本发明涉及将水溶性聚合物与治疗性蛋白质的氧化碳水化合物部分缀合的材料和方法，包括在允许缀合的条件下使氧化的碳水化合物部分与活化的水溶性聚合物接触。 更具体地说，本发明涉及上述材料和方法，其中水溶性聚合物含有活性氨基氧基，其中在水溶性聚合物上的氧化碳水化合物部分和活性氨基氧基之间形成肟或腙键，其中 共轭在亲核催化剂存在下进行。
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公开(公告)号：EP2459226A2
公开(公告)日：2012-06-06
申请号：EP10739386.0
申请日：2010-07-26
发明人： JAIN, Sanjay , GREGORIADIS, Gregory , DWIVEDI, Archana , NATH, Srijit , SIEKMANN, Juergen , HAIDER, Stefan , ROTTENSTEINER, Hanspeter , TURECEK, Peter
IPC分类号： A61K47/48
CPC分类号： A61K47/6455 , A61K47/60 , A61K47/61 , A61K47/65
摘要： A water soluble polymer, in particular polysialic acid (PSA) or a modified PSA (mPSA), is conjugated to an oxidized carbohydrate moiety of a glycoprotein other than a blood coagulation protein or to a ganglioside or drug delivery system by contacting the oxidized carbohydrate moiety with the water soluble polymer, wherein said water soluble polymer contains an aminooxy group and an oxime linkage is formed between the oxidized carbohydrate moiety and the aminooxy group on the water soluble polymer or wherein said water soluble polymer contains a hydrazide group and a hydrazone linkage is formed between the oxidized carbohydrate moiety and the hydrazide group on the water soluble polymer. Conjugates of aminooxy- or hydrazide-water soluble polymer, such as PSA and mPSA, are thus obtained in which the PSA or mPSA is attached via a carbohydrate moiety.
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3.发明公开METHODS OF MEASURING ADAMTS13-MEDIATED IN VIVO CLEAVAGE OF VON WILLEBRAND FACTOR AND USES THEREOF 有权值的测定方法ADAMTS13介导体内切割维勒布兰德因子及其用途
公开(公告)号：EP2358895A1
公开(公告)日：2011-08-24
申请号：EP09764426.4
申请日：2009-12-03
发明人： VARADI, Katalin , ROTTENSTEINER, Hanspeter , TURECEK, Peter , SCHWARZ, Hans-Peter , SCHREINER, Jutta
IPC分类号： C12Q1/37
CPC分类号： G01N33/86 , C12Q1/37 , C12Q1/56 , G01N33/573 , G01N2333/755 , G01N2333/96486 , G01N2800/224 , G01N2800/52
摘要： The invention generally relates to methods of measuring cleaved von Willebrand factor (VWF) fragments. More specifically, the invention relates to methods of measuring the ability of a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13 (ADAMTS13) to cleave VWF in vivo. The invention also relates to methods of using various animal models which demonstrate ADAMTS13 activity similar to that of a human. The invention further relates to methods of measuring the cleavage products of rVWF in mammals, particularly in humans and in human plasma.
摘要翻译： 本发明基因的反弹涉及测量裂解von Willebrand因子（VWF）的片段的方法。 更具体地，本发明涉及到测量整合素和金属蛋白酶的具有血小板反应类型1基序的，构件13（ADAMTS13）切割VWF体内的能力的方法。 因此，本发明涉及使用多种动物模型的方法演示了类似于做了人体的哪个ADAMTS13活性。 本发明还涉及到测量的r的vWF的切割产物在哺乳动物，特别是在人类和在人血浆中的方法。
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公开(公告)号：EP2849795A1
公开(公告)日：2015-03-25
申请号：EP13728026.9
申请日：2013-05-16
IPC分类号： A61K47/48
摘要： The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
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5.发明公开NUCLEOPHILIC CATALYSTS FOR OXIME LINKAGE AND USE OF NMR ANALYSES OF THE SAME 审中-公开亲核催化剂结合和使用肟的NMR分析可知：
公开(公告)号：EP2849794A1
公开(公告)日：2015-03-25
申请号：EP13724491.9
申请日：2013-05-16
发明人： RAY, G., Joseph , ZHANG, Zhenqing , SZABO, Christina , SIEKMANN, Juergen , SCHEINECKER, Richard , HAIDER, Stefan , ROTTENSTEINER, Hanspeter , KOSMA, Paul
IPC分类号： A61K47/48
摘要： The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a therapeutic protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation and analyzing the conjugation using 2D NMR analysis. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime or hydrazone linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer, and wherein the conjugation is carried out in the presence of a nucleophilic catalyst.
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6.发明公开MATERIALS AND METHODS FOR CONJUGATING A WATER SOLUBLE FATTY ACID DERIVATIVE TO A PROTEIN 审中-公开材料和方法水溶性脂肪酸衍生物的蛋白质的共轭
公开(公告)号：EP2654794A1
公开(公告)日：2013-10-30
申请号：EP11806103.5
申请日：2011-12-16
摘要： The invention relates to materials and methods of conjugating a water soluble fatty acid derivative to a therapeutic protein comprising contacting the therapeutic protein with an activated water soluble fatty acid derivative under conditions that allow conjugation.
摘要翻译： 本发明涉及的材料和缀合的水溶性脂肪酸衍生物对治疗性蛋白，其包含与在活化的水溶性脂肪酸衍生物接触所述治疗性蛋白质的条件下确实允许缀合的方法。
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公开(公告)号：EP2459224A2
公开(公告)日：2012-06-06
申请号：EP10738119.6
申请日：2010-07-26
IPC分类号： A61K47/48
CPC分类号： A61K47/4823 , A61K38/37 , A61K38/4846 , A61K47/60 , A61K47/61 , C08B37/0006 , C12N9/6437 , C12N9/644 , C12N9/96 , C12Y304/21021 , C12Y304/21022
摘要： The invention relates to materials and methods of conjugating a water soluble polymer to an oxidized carbohydrate moiety of a blood coagulation protein comprising contacting the oxidized carbohydrate moiety with an activated water soluble polymer under conditions that allow conjugation. More specifically, the present invention relates to the aforementioned materials and methods wherein the water soluble polymer contains an active aminooxy group and wherein an oxime linkage is formed between the oxidized carbohydrate moiety and the active aminooxy group on the water soluble polymer. In one embodiment of the invention the conjugation is carried out in the presence of the nucleophilic catalyst aniline. In addition the generated oxime linkage can be stabilized by reduction with NaCNBH
3 to form an alkoxyamine linkage.摘要翻译： 本发明涉及使水溶性聚合物与血液凝固蛋白的氧化碳水化合物部分缀合的材料和方法，包括使氧化碳水化合物部分与活化的水溶性聚合物在允许缀合的条件下接触。 更具体地说，本发明涉及上述材料和方法，其中水溶性聚合物含有活性氨氧基，并且其中在水溶性聚合物上的氧化碳水化合物部分和活性氨氧基之间形成肟键。 在本发明的一个实施方案中，缀合在亲核催化剂苯胺存在下进行。 另外，生成的肟键可以通过用NaCNBH 3还原形成烷氧基胺键来稳定。
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公开(公告)号：EP2710377B1
公开(公告)日：2015-09-09
申请号：EP12725948.9
申请日：2012-05-18
发明人： FERRARI, Silvia , GRUBER, Bernadette , PLAIMAUER, Barbara , ROTTENSTEINER, Hanspeter , SCHEIFLINGER, Friedrich
IPC分类号： G01N33/564
CPC分类号： G01N33/564
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公开(公告)号：EP2710377A1
公开(公告)日：2014-03-26
申请号：EP12725948.9
申请日：2012-05-18
发明人： FERRARI, Silvia , GRUBER, Bernadette , PLAIMAUER, Barbara , ROTTENSTEINER, Hanspeter , SCHEIFLINGER, Friedrich
IPC分类号： G01N33/564
CPC分类号： G01N33/564
摘要： The present invention relates to methods and means for detecting ADAMTS13 immune complexes in a sample. The methods include the steps of capturing and labelling immune complexes of anti-ADAMTS13 antibodies. Capturing and labelling may be achieved by two different binding units targeting the immune complexes. The invention further relates to diagnosing diseases associated with immunologic ADAMTS13 dysfunction like TTP (thrombotic thrombocy-topenic purpura).
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10.发明公开THERAPEUTIC PROTEINS WITH INCREASED HALF-LIFE AND METHODS OF PREPARING SAME 审中-公开提出增强半衰期和过程的治疗性蛋白质及其
公开(公告)号：EP2714093A1
公开(公告)日：2014-04-09
申请号：EP12725992.7
申请日：2012-05-25
摘要： The present disclosure relates to materials and methods of conjugating a water soluble polymer to a therapeutic protein.
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