摘要:
The invention concerns the use of a miniature device for separating and isolating biological objects for various applications in the field of molecular biology, methods for making DNA or protein chips in particular, and the DNA or protein chips obtained by using said methods.
摘要:
The invention concerns the use of a miniature device for separating and isolating biological objects for various applications in the field of molecular biology, methods for making DNA or protein chips in particular, and the DNA or protein chips obtained by using said methods.
摘要:
The invention concerns a miniature device for separating and isolating biological objects, the use of said device for isolating, separating, culturing and/or analysing biological objects and a method for separating and isolating biological objects using said device.
摘要:
The invention concerns a method for detecting a known function, from nucleic acids present in a sample, characterised in that it comprises the following steps: (a) preparing, from the nucleic acids of the sample, nucleic acid molecules comprising the gene(s) coding for the protein(s) corresponding to said function, and the control elements required for the transcription and the translation of said gene(s); (b) in vitro transcription and translation of the nucleic acid molecule prepared in step (a); (c) detecting and/or measuring the function corresponding to the protein(s) produced in step (b).
摘要:
The invention concerns a method for separating and characterising functions potentially present in a biological sample containing nucleic acids, characterised in that it comprises the following steps: (a) preparing nucleic acid fragments from said sample; (b) associating each of said fragments with a carrier molecule; (c) isolating each fragment or a part of each construct formed by a fragment associated with a carrier molecule; (d) treating in vitro each fragment or a part of each construct formed by a fragment associated with a carrier molecule isolated at step (c) to obtain transcripts; (e) testing a function of the transcripts obtained at step (d) or proteins for which they code after said transcripts have been translated.
摘要:
The invention concerns a method for producing in vitro recombined polynucleotide sequences from a bank of polynucleotide sequences, characterised in that it comprises the following steps: fragmenting an initial bank of double-strand polynucleotide sequences; (b) denaturation of the fragments resulting from step (a) optionally in the presence of one or several assembling matrix or matrices; (c) hybridising said fragments with one or several of the assembling matrix or matrices if the latter is/are not present in step (b); (d) ligation of said fragments; (e) optionally cloning said recombined polynucleotide sequences having advantageous properties with respect to one or several reference sequences.
摘要:
Method of preparing fragments for shuffling using DNA repair enzymes. Repair enzymes can be used to repair damaged and mismatched residues to reduce level of mutation in fragments, or can themselves create fragments when used without sufficient DNA polymerase. Invention further includes reaction mixtures created by or during the method, sequences created by the method, hosts and vectors containing same, and proteins translated therefrom.
摘要:
La présente invention concerne une méthode de détection d'une fonction connue, à partir des acides nucléiques présents dans un échantillon, caractérisée en ce qu'elle comprend les étapes suivantes (a) la préparation, à partir des acides nucléiques de l'échantillon, de molécules d'acides nucléiques comprenant le ou les gènes codant pour la ou les protéines correspondant à ladite fonction, et les éléments de contrôle nécessaires à la transcription et à la traduction du ou desdits gènes; (b) la transcription et la traduction in vitro des molécules d'acide nucléique préparée(s) à l'étape (a); (c) la détection et/ou la mesure de la fonction correspondant à la ou aux protéines produites à l'étape (b).
摘要:
The invention concerns a method for determining the activity in vitro of a substance using a functional test, characterised in that it consists in detecting and/or measuring the variation of a known function corresponding to one or several proteins produced in vitro in the presence and in the absence of said substance or to the substance in the presence or in the absence of one or several proteins produced in vitro.
摘要:
La présente invention concerne une méthode de détection d'une fonction connue, à partir des acides nucléiques présents dans un échantillon, caractérisée en ce qu'elle comprend les étapes suivantes (a) la préparation, à partir des acides nucléiques de l'échantillon, de molécules d'acides nucléiques comprenant le ou les gènes codant pour la ou les protéines correspondant à ladite fonction, et les éléments de contrôle nécessaires à la transcription et à la traduction du ou desdits gènes; (b) la transcription et la traduction in vitro des molécules d'acide nucléique préparée(s) à l'étape (a); (c) la détection et/ou la mesure de la fonction correspondant à la ou aux protéines produites à l'étape (b).