摘要:
The invention concerns a device (6) for sampling molecules of biological interest. Said device (6) comprises capturing zones (10) whereof the developed surface is much greater than the surface such that the contact sampling enables a sufficient amount of molecules to be obtained to achieve a reliable analysis, while being of dimensions such that the device (6) is neither invasive nor aggressive. In particular the device comprises stepped capture zones (10) localized on a support (12) made according to microtechnology techniques; the capture zones (10) can advantageously be functionalized.
摘要:
The invention concerns a method for transporting an analyte present in a sample, a method for concentrating an analyte present in a sample, and a device for implementing said methods. The method for transporting an analyte present in a sample consists in preparing a solution A from the sample wherein the analyte is fixed on magnetic particles; introducing solution A in a first container connected via a bottle-neck to a second container; displacing with a magnetic system the analyte fixed on magnetic particles from the first container to the second container via the bottle-neck; the second container being filled with all or part of solution A and/or with another solution.
摘要:
The invention concerns a method and a device for testing an electronic chip (10) comprising at its surface a plurality of addressable electrically conductive sensitive elements (12) which consists in contacting with a conducting solution (52) the chip sensitive elements; in selectively addressing one or several sensitive elements (12) for applying to each sensitive element addressed a testing electric signal, called input signal, and in measuring a signal, called output signal, on an electrode (60, 60a) also in contact with the conducting solution.
摘要:
The invention concerns a method for making a biochip and a biochip, said biochip consisting in particular of biological probes grafted on an conductive polymer. The inventive method comprises the following steps: a) structuring a substrate so as to obtain on said substrate microwells comprising at their base a layer of a material capable of initiating and promoting adhesion thereon of a copolymer film of pyrrole and pyrrole functionalised by electropolymerisation; b) collective electropolymerisation so as to form an electropolymerised film of a copolymer of pyrrole or functionalised pyrrole on the base of said microwells; c) directly or indirectly fixing functionalised oligonucleotides by micro-deposition or liquid jet printing process.
摘要:
Process for the production of a seal providing mechanical strength between a substrate and a chip hybridized by beads on the substrate. The invention concerns a process for producing an encapsulating seal for sealing a chip (1) hybridized by beads on a substrate (5) and providing said chip with mechanical strength. The process consists in, parallel to the production of hybridation beads (9) on the chip or substrate underside (1a) using a first fusible material: (a) depositing, on the substrate or on the underside of the electronic component, a seal (13) made of a second fusible material; (b) placing the underside of the chip on the substrate so as to provide connections between the chip and the substrate by means of the first fusible material; and (c) heating the whole so formed at a temperature at least equal to the highest melting temperature of the first and second fusible materials, so as to produce hybridation beads using the first material and the seal using the second material. The height (h) and width (d) of the seal are based on the expressions (I) where α is a shape factor and D is the largest dimension of the electronic component.
摘要:
The invention concerns the use of a miniature device for separating and isolating biological objects for various applications in the field of molecular biology, methods for making DNA or protein chips in particular, and the DNA or protein chips obtained by using said methods.
摘要:
The invention relates to a microsystem which is intended to receive beads and to position said beads precisely at determined locations in the microsystem. The inventive microsystem consists of a container (3) comprising a cavity (4) which is equipped with blocking elements (5). According to the invention, the blocking elements are used to arrange and stack the beads in the spaces therebetween, said spaces forming the aforementioned determined locations. The invention also comprises a cover (7) which is hermetically fixed to the container (3) and inlet (8) and outlet (9) means which enable a fluid to flow into the cavity (4). The invention also relates to the production and use of said bead-filled microsystem.
摘要:
The invention relates to an in vivo diagnostic and therapy micro-device, comprising an essentially long body, preferably with parallel faces and, in the longitudinal direction thereof, at least one principal channel (24), one inlet (18) of which is located at a first end (14) of the body.
摘要:
The invention concerns a working device characterized in that it comprises an active surface (Sa) substantially non wetting with respect to a liquid of interest; at least one localized capture zone (Zc) of a drop of said liquid, formed on said active surface; at least one working zone (Zt) arranged with a capture zone such that the working zone is covered at least partly by a drop of the liquid when the latter is captured by said capture zone; and means for allowing a drop of said liquid to remain on said capture zone. Said device enables in particular high density matrices of drops of said liquid to be formed on a surface, in particular for performing chemical or biochemical reactions and/or for analyzing the liquid of interest at each drop. The invention is applicable in biological chips.
摘要:
The invention concerns a platform for chemical or biological analysis (102) comprising at least a fixed support (110) and at least a first mobile support (104a) capable of being lined with reagent, the mobile support being linked to the fixed support by first flexible retaining means (108a) capable of bending in response to a modification in a weight supported by the first mobile support, brought about by a chemical or biological reaction of said reagent.