公开(公告)号：EP2828385A1

公开(公告)日：2015-01-28

申请号：EP13765270.7

申请日：2013-03-22

申请人： Codexis, Inc.

发明人： NAZOR, Jovana ,  SMITH, Derek ,  CROWE, Michael, A. ,  SONG, Shiwei ,  COLLIER, Steven, J.

IPC分类号： C12N9/10

CPC分类号： C12N9/1096 ,  C12P17/10 ,  C12Y206/01 ,  C12Y206/01018 ,  Y02P20/52

摘要： The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered polypeptides having transaminase activity, polynucleotides encoding the polypeptides, methods of the making the polypeptides, and methods of using the polypeptides for the biocatalytic conversion of ketone substrates to amine products. The present enzymes have been engineered to have one or more residue differences as compared to the amino acid sequence of the naturally occurring transaminase of Vibrio fluvialis. In particular, the transaminases of the present disclosure have been engineered for efficient formation of chiral tryptamine derivatives from its corresponding prochiral ketone substrates.

公开(公告)号：EP2780448A1

公开(公告)日：2014-09-24

申请号：EP12795954.2

申请日：2012-11-14

申请人： Codexis, Inc.

发明人： CABIROL, Fabien ,  CHEN, Haibin ,  GOHEL, Anupam ,  COLLIER, Steven, J. ,  SMITH, Derek ,  KOSJEK, Birgit ,  JANEY, Jacob

IPC分类号： C12N9/04 ,  C12P13/00 ,  C12P7/00 ,  C12P17/10 ,  C12N9/00

CPC分类号： C12N9/0006 ,  C12N15/70 ,  C12P13/00 ,  C12P13/001 ,  C12P13/02 ,  C12P41/002 ,  C12Y101/01184 ,  Y02P20/52

摘要： The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides.

摘要翻译： 本公开涉及用于制备羟基取代的氨基甲酸酯化合物的工程化酮还原酶多肽，以及多核苷酸，载体，宿主细胞，以及制备和使用酮还原酶多肽的方法。

公开(公告)号：EP2697662B1

公开(公告)日：2018-06-06

申请号：EP12771861.7

申请日：2012-04-12

申请人： Codexis, Inc.

发明人： GOHEL, Anupam ,  SMITH, Derek ,  WONG, Brian ,  SUKUMARAN, Joly ,  YEO, Wan Lin ,  COLLIER, Steven, J. ,  NOVICK, Scott

IPC分类号： A01N43/00 ,  A61K31/55 ,  C12N9/02

CPC分类号： C12P17/10 ,  C07D223/22 ,  C07D223/28 ,  C07D453/02 ,  C12N9/0006 ,  C12N9/004 ,  C12N11/00 ,  C12P41/002 ,  C12Y101/00 ,  Y02P20/52

摘要： The present disclosure relates to biocatalysts and its uses for the efficient preparation of eslicarbazepine, eslicarbazepine acetate, and analogs thereof.

公开(公告)号：EP2649187B1

公开(公告)日：2017-11-22

申请号：EP11846568.1

申请日：2011-12-07

申请人： Codexis, Inc.

发明人： ANG, Ee Lui ,  CLAY, Michael, D. ,  BEHROUZIAN, Behnaz ,  EBERHARD, Ellen ,  COLLIER, Steven, J. ,  FU FAN, Jasyln ,  SMITH, Derek ,  SONG, Shiwei ,  ALVIZO, Oscar ,  WIDEGREN, Magnus ,  ZHU, Jun ,  XU, Junye ,  WILSON, Robert

IPC分类号： C12P13/02 ,  C07K1/00

CPC分类号： C12N9/0073 ,  C12P11/00 ,  C12P13/02 ,  C12P41/002 ,  C12Y114/13022 ,  Y02P20/52

摘要： The present invention relates to non-naturally occurring polypeptides useful for preparing armodafinil, polynucleotides encoding the polypeptides, and methods of using the polypeptides. The non-naturally occurring polypeptides of the present invention are effective in carrying out biocatalytic conversion of the (i) 2-(benzhydrylsulfinyl)acetamide to (−)-2-[(R)-(diphenylmethyl)sulfinyl]acetamide (armodafinil), or (ii) benzhydryl-thioacetic acid to (R)-2-(benzhydrylsulfinyl)acetic acid, which is a pivotal intermediate in the synthesis of armodafinil, in enantiomeric excess.

公开(公告)号：EP2606139A2

公开(公告)日：2013-06-26

申请号：EP11818555.2

申请日：2011-08-08

申请人： Codexis, Inc. ,  Cabirol, Fabien L. ,  Gohel, Anupam ,  Collier, Steven J. ,  Liang, Jack ,  Mock, Marissa ,  Mundorff, Emily ,  Novick, Scott ,  Limanto, John ,  Smith, Derek ,  Beutner, Gregory ,  Klapars, Artis ,  Ashley, Eric ,  Strotman, Hallena ,  Truppo, Matthew ,  Hughes, Gregory ,  Grau, Brendan ,  Janey, Jacob

发明人： CABIROL, Fabien, L. ,  GOHEL, Anupam ,  COLLIER, Steven, J. ,  LIANG, Jack ,  MOCK, Marissa ,  MUNDORFF, Emily ,  NOVICK, Scott ,  LIMANTO, John ,  SMITH, Derek ,  BEUTNER, Gregory ,  KLAPARS, Artis ,  ASHLEY, Eric ,  STROTMAN, Hallena ,  TRUPPO, Matthew ,  HUGHES, Gregory ,  GRAU, Brendan ,  JANEY, Jacob

IPC分类号： C12P13/00 ,  C12P7/00

CPC分类号： C12P13/001 ,  C12N9/1096

摘要： The disclosure provides transaminase polypeptides capable of converting the substrate, 2-(3,4-dimethoxyphenethoxy)cyclohexanone to the trans diastereomer product (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine in at least a 2:1 diastereomeric ratio relative to the cis diastereomer (1R,2S)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine. The disclosure also provides polynucleotides, vectors, host cells, and methods of making and using the transaminase polypeptides in processes for preparing (1R,2R)-2-(3,4-dimethoxyphenethoxy)cyclohexanamine and its analogs, which can product compounds can be further used to prepare the aminocyclohexylether compound, (3R)-1-[(1R,2R)-2-[2-(3,4-dimethoxyphenyl)ethoxy]cyclohexyl]pyrrolidin-3-ol, which is an ion channel blocker.

摘要翻译： 本公开提供了能够以至少2:1非对映异构体将底物2-（3,4-二甲氧基苯乙氧基）环己酮转化成反式非对映异构体产物（1R，2R）-2-（3,4-二甲氧基苯乙氧基）环己胺的转氨酶多肽 相对于顺式非对映异构体（1R，2S）-2-（3,4-二甲氧基苯乙氧基）环己胺的比例。 本公开还提供了用于制备（1R，2R）-2-（3,4-二甲氧基苯乙氧基）环己胺及其类似物的方法中制备和使用转氨酶多肽的多核苷酸，载体，宿主细胞和方法， 进一步用于制备作为离子通道阻断剂的氨基环己基醚化合物（3R）-1 - [（1R，2R）-2- [2-（3,4-二甲氧基苯基）乙氧基]环己基]吡咯烷-3-醇。

公开(公告)号：EP2566497A1

公开(公告)日：2013-03-13

申请号：EP11778262.3

申请日：2011-05-04

申请人： Codexis, Inc.

发明人： CROWE, Michael, A. ,  ALVIZO, Oscar ,  BEHROUZIAN, Behnaz ,  BONG, Yong Koy ,  COLLIER, Steven, J. ,  GOHEL, Anupam ,  MAVINAHALLI, Jagadeesh ,  MODUKURU, Naga ,  MUNDORFF, Emily ,  SMITH, Derek ,  SONG, Shiwei ,  YEO, Wan Lin

IPC分类号： A61K38/16 ,  C07K1/00

CPC分类号： C12N9/0006 ,  A61K38/00 ,  C07K14/47 ,  C12P17/10 ,  C12P17/14 ,  C12Y101/00 ,  C12Y101/01184

摘要： The present disclosure relates to non-naturally occurring polypeptides useful for preparing Ezetimibe, polynucleotides encoding the polypeptides, and methods of using the polypeptides.

摘要翻译： 本公开涉及用于制备依泽替米贝的非天然存在的多肽，编码所述多肽的多核苷酸以及使用所述多肽的方法。

公开(公告)号：EP2329013A2

公开(公告)日：2011-06-08

申请号：EP09810573.7

申请日：2009-08-27

申请人： Codexis, Inc.

发明人： SAVILE, Christopher ,  GRUBER, John, M. ,  MUNDORFF, Emily ,  HUISMAN, Gjalt, W. ,  COLLIER, Steven, J.

IPC分类号： C12N9/02 ,  C12N15/53 ,  C12P7/00

CPC分类号： C12N9/0006 ,  C12P13/001 ,  C12P17/00 ,  C12P41/002

摘要： The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. The engineered ketoreductase polypeptides are optimized for catalyzing the conversion of
N,N -dimethyl-3-keto-3-(2-thienyl)-1-ketopropanamine to (
S )
-N,N -dimethyl-3-hydroxy-3-(2-thienyl)-1-propanamine.

摘要翻译： 本公开提供了与天然存在的野生型酮还原酶相比具有改善的性质的工程酮还原酶。 还提供了编码工程酮还原酶的多核苷酸，能够表达工程酮还原酶的宿主细胞，以及使用工程酮还原酶合成各种手性化合物的方法。 改造的工程酮还原酶多肽用于催化N，N-二甲基-3-酮基-3-（2-噻吩基）-1-酮丙胺转化为（S）-N，N-二甲基-3-羟基-3-（ 2-噻吩基）-1-丙胺。

公开(公告)号：EP2847327B1

公开(公告)日：2018-12-26

申请号：EP13788385.6

申请日：2013-05-07

申请人： Codexis, Inc.

发明人： CHEN, Haibin ,  BONG, Yong Koy ,  CABIROL, Fabien ,  GOHEL, Anupam ,  LI, Tao ,  MOORE, Jeffrey C. ,  QUINTANAR-AUDELO, Martina ,  YANG, Hong ,  COLLIER, Steven, J. ,  SMITH, Derek

IPC分类号： C12N9/04 ,  C12N5/00 ,  C12P17/18 ,  C12N9/02 ,  C12P13/04 ,  C12P13/24 ,  C12P17/12

CPC分类号： C12P17/12 ,  C12N9/0071 ,  C12P13/04 ,  C12P13/24 ,  C12P17/188 ,  C12Y114/11002 ,  Y02P20/52

摘要： The present disclosure provides engineered proline hydroxylase polypeptides for the production of hydroxylated compounds, polynucleotides encoding the engineered proline hydroxylases, host cells capable of expressing the engineered proline hydroxylases, and methods of using the engineered proline hydroxylases to prepare compounds useful in the production of active pharmaceutical agents. The present disclosure provides engineered proline hydroxylase biocatalysts, polynucleotides encoding the biocatalysts, methods of their preparation, and processes for preparing hydroxylated compounds using these engineered biocatalysts.

公开(公告)号：EP2828385B1

公开(公告)日：2018-02-07

申请号：EP13765270.7

申请日：2013-03-22

申请人： Codexis, Inc.

发明人： NAZOR, Jovana ,  SMITH, Derek ,  CROWE, Michael, A. ,  SONG, Shiwei ,  COLLIER, Steven, J.

IPC分类号： C12N9/10

CPC分类号： C12N9/1096 ,  C12P17/10 ,  C12Y206/01 ,  C12Y206/01018 ,  Y02P20/52

摘要： The present disclosure provides engineered transaminase polypeptides for the production of amines, polynucleotides encoding the engineered transaminases, host cells capable of expressing the engineered transaminases, and methods of using the engineered transaminases to prepare compounds useful in the production of active pharmaceutical agents.

公开(公告)号：EP2467473B1

公开(公告)日：2016-03-23

申请号：EP10810597.4

申请日：2010-08-19

申请人： Codexis, Inc.

发明人： ALVIZO, Oscar ,  COLLIER, Steven, J. ,  HENNEMANN, Joerg ,  OH, Seong, Ho ,  ZHA, Wenjuan

IPC分类号： C12N9/04 ,  C12N15/53 ,  C12P13/00 ,  A61K31/137

CPC分类号： C12N9/0006 ,  A61K31/137 ,  C12P13/001 ,  C12Y101/01184

摘要： The disclosure relates to engineered ketoreductase polypeptides and processes of using the polypeptides for production of phenylephrine.