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    ENZYMATIC OIL-DEGUMMING METHOD
    3.
    发明授权
    ENZYMATIC OIL-DEGUMMING METHOD 有权
    ENZYMATIC OIL-DEGUMMING法

    公开(公告)号：EP1791933B1

    公开(公告)日：2011-06-29

    申请号：EP05761577.5

    申请日：2005-07-18

    申请人： DANISCO A/S

    发明人： SOE, Jorn, Borch ,  TURNER, Mark

    IPC分类号： C11B3/00 ,  C11C3/00 ,  C07F9/10

    CPC分类号： C12N9/1029 ,  A21D8/042 ,  A23J7/00 ,  C11B3/003 ,  C12N9/20 ,  C12P7/6418 ,  C12P7/6445 ,  C12P7/6481 ,  C12Y301/01003

    摘要： A process of enzymatic degumming edible oils, comprising treating the edible oil with a lipid acyltransferase so as to transfer an acyl group from a major part of the phospholipid to one or more acyl acceptors, wherein the acyl acceptor may be any compound comprising a hydroxy group. In one embodiment preferably the acyl acceptor is water and in another embodiment preferably the acyl acceptor is one or more sterols and/or stanols. When the acyl acceptor is a stanol and/or sterol, one or more sterol esters and/or stanol esters are produced. The lipid acyltransferase for use in the process of the present invention may comprises one or more of the following amino acid sequences: SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 4, SEQ ID No. 5, SEQ ID No. 6, SEQ ID No. 7, SEQ ID No. 8, SEQ ID No. 9, SEQ ID No. 10, SEQ ID No. 11, SEQ ID No. 12, SEQ ID No. 13, SEQ ID No. 14, or SEQ ID No. 15, SEQ ID No. 16, SEQ ID No. 17, SEQ ID No. 18, SEQ ID No. 36, SEQ ID No. 38, SEQ ID No. 40, SEQ ID No. 41, SEQ ID No 45, SEQ ID No. 47, SEQ ID No. 50 or an amino acid sequence which has 75% or more identity thereto. A novel lipid acyltransferase comprising the amino acid sequence shown as SEQ ID No. 16 is also taught.

    摘要翻译： 一种酶促脱胶食用油的方法，包括用脂质酰基转移酶处理食用油以便将酰基从大部分磷脂转移到一个或多个酰基受体，其中酰基受体可以是任何含有羟基的化合物 。 在一个实施方案中，优选酰基受体是水，在另一个实施方案中，优选酰基受体是一种或多种甾醇和/或甾烷醇。 当酰基受体是甾烷醇和/或甾醇时，产生一种或多种甾醇酯和/或甾烷醇酯。 用于本发明方法的脂质酰基转移酶可以包含一个或多个以下氨基酸序列：SEQ ID No.1，SEQ ID No.3，SEQ ID No.4，SEQ ID No.5，SEQ ID No.5 序列号6，序列号7，序列号8，序列号9，序列号10，序列号11，序列号12，序列号13，序列号 14或SEQ ID No.15，SEQ ID No.16，SEQ ID No.17，SEQ ID No.18，SEQ ID No.36，SEQ ID No.38，SEQ ID No.40，SEQ ID No.41 ，SEQ ID No 45，SEQ ID No.47，SEQ ID No.50或与其具有75％或更多同一性的氨基酸序列。 还教导了包含如SEQ ID No.16所示的氨基酸序列的新型脂质酰基转移酶。

    FUNGAL LIPOLYTIC ENZYMES
    6.
    发明公开
    FUNGAL LIPOLYTIC ENZYMES 有权
    真菌脂肪分解酶

    公开(公告)号：EP1722636A2

    公开(公告)日：2006-11-22

    申请号：EP05718349.3

    申请日：2005-03-10

    申请人： DANISCO A/S

    发明人： BRUNSTEDT, Janne ,  MIKKELSEN, Jorn, Dalgaard ,  PEDERSEN, Henrik ,  SOE, Jorn, Borch

    IPC分类号： A21D8/04 ,  C12N9/18 ,  C12N9/20

    CPC分类号： A21D8/042 ,  A23C19/0328 ,  A23D7/02 ,  A23K20/189 ,  A23K50/75 ,  A23L7/107 ,  A23L7/109 ,  A23L7/111 ,  A23L27/60 ,  A23L29/06 ,  C11B3/003 ,  C12N9/18 ,  C12P7/62 ,  C12P19/44 ,  C12Y301/01

    摘要： A fungal wild-type lipolytic enzyme having a higher ratio of activity on polar lipids compared with triglycerides, wherein the enzyme preferably has a phospholipidAriglyceride hydrolysing activity ratio of at least 4. Preferably, the lipolytic enzyme according to the present invention has a glycolipid:triglyceride hydrolysing activity ratio of at least 1.5. In one embodiment, the fungal lipolytic enzyme according to the present invention comprises an amino acid sequence as shown in SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 4 or SEQ ID No. 6 or an amino acid sequence which has at least 90% identity thereto. The present invention further encompasses a nucleic acid encoding a fungal lipolytic enzyme, which nucleic acid is selected from the group consisting of: (a) a nucleic acid comprising a nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7; (b) a nucleic acid which is related to the nucleotide sequence of SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7 by the degeneration of the genetic code; and (c) nucleic acid comprising a nucleotide sequence which has at least 90% identity with the nucleotide sequence shown in SEQ ID No. 3, SEQ ID No. 5 or SEQ ID No. 7.

    SOLID PHASE GLYCEROLYSIS
    9.
    发明公开
    SOLID PHASE GLYCEROLYSIS 有权
    固相磷化

    公开(公告)号：EP1304925A2

    公开(公告)日：2003-05-02

    申请号：EP01972384.0

    申请日：2001-08-02

    申请人： DANISCO A/S

    发明人： SOE, Jorn, Borch

    IPC分类号： A21D13/00 ,  A23C13/00 ,  A23G3/00 ,  A23G9/00 ,  A23L1/035 ,  A23D7/00 ,  C12P7/64 ,  C11C3/06

    CPC分类号： C12P7/6445 ,  A21D2/16 ,  A23D7/011 ,  A23D7/013 ,  A23D9/013 ,  C11C3/06

    摘要： An enzymatic solid phase reaction for preparing a solid having greater than 40 % monoglyceride from a reaction mixture; wherein the reaction mixture comprises: (i) lipase; (ii) at least 14 weight % glycerol; and (iii) glyceride; and optionally (iv) lecithin; such that if (iv) is not present then the glyceride (iii) has an iodine value of between about 5 and about 35, and a solid fat content of more than about 75 % at 20 °C. The present invention further relates to a process of preparing an enzymatic solid phase reaction mixture for preparing a solid having greater than 40 % monoglyceride.

    摘要翻译： 用于从反应混合物制备具有大于40％甘油一酸酯的固体的酶促固相反应; 其中所述反应混合物包含：（i）脂肪酶; （ii）至少14重量％的甘油; 和（iii）甘油酯; 和任选地（iv）卵磷脂; 使得如果（iv）不存在，那么甘油酯（iii）具有介于约5与约35之间的碘值以及在20℃下大于约75％的固体脂肪含量。 本发明进一步涉及制备酶固相反应混合物用于制备具有大于40％甘油单酯的固体的方法。