公开(公告)号：EP2788494A1

公开(公告)日：2014-10-15

申请号：EP12784273.0

申请日：2012-11-14

申请人： Evonik Degussa GmbH

发明人： HAAS, Thomas ,  SCHAFFER, Steffen ,  PÖTTER, Markus ,  WESSEL, Mirja ,  PFEFFER, Jan, Christoph ,  GEHRING, Christian ,  KIRCHNER, Nicole ,  Wittmann, Eva, Maria

IPC分类号： C12P7/42 ,  C12N15/52 ,  C12N9/02

CPC分类号： C12P7/42 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/1217 ,  C12N9/13 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12Y103/99002 ,  C12Y207/02007 ,  C12Y402/01017 ,  C12Y602/01002

摘要： The invention relates to a method having the steps of a) providing isobutyric acid, b) bringing the isobutyric acid into contact with the combination of isobutyrate kinase and phosphotransisobutyrylase and/or isobutyryl-coenzyme A-synthetase/ligase and/or isobutyrate-coenzyme A-transferase, c) bringing the product of step a) into contact with isobutyryl-coenzyme A-dehydrogenase, d) bringing the product of step b) into contact with methacrylyl-coenzyme A-hydratase, and e) hydrolyzing the product of step d) with the formation of 3-hydroxyisobutyric acid. At least one of the enzymes is used in the form of a cell which comprises an activity of 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, said activity being reduced with respect to the wild type of the cell. The invention also relates to a cell which comprises at least one enzyme of the group comprising isobutyryl-coenzyme A-synthetase/ligase, isobutyrate-coenzyme A-transferase, isobutyrate-kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A-dehydrogenase, methacrylyl-coenzyme A-hydratase, and 3-hydroxisobutyryl-coenzyme A-hydrolase and which has an activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, said activity being reduced with respect to the wild type of the cell. Additionally, the cell preferably has a monooxygenase, even more preferably a monooxygenase of the AlkBGT type or a variant thereof. The invention also relates to the use of such a cell for producing 3-hydroxyisobutyric acid

公开(公告)号：EP2744819A1

公开(公告)日：2014-06-25

申请号：EP12747926.9

申请日：2012-08-15

申请人： Evonik Degussa GmbH

发明人： SCHAFFER, Steffen ,  GIELEN, Jasmin ,  DECKER, Nicole ,  KIRCHNER, Nicole ,  HAAS, Thomas ,  PÖTTER, Markus ,  HÄGER, Harald

IPC分类号： C07K14/21 ,  C12N9/00 ,  C12P7/62

CPC分类号： C12P7/649 ,  C07K14/21 ,  C12P5/026 ,  C12P7/62 ,  C12P7/64 ,  C12P7/6409 ,  C12P13/001 ,  Y02E50/343 ,  Y02T50/678

摘要： The invention relates to a biotechnological process for the preparation of organic compounds with the aid of at least one alkL gene product.

公开(公告)号：EP3292207A1

公开(公告)日：2018-03-14

申请号：EP16722561.4

申请日：2016-04-28

申请人： Evonik Degussa GmbH

发明人： GRAMMANN, Katrin ,  HENNEMANN, Hans-Georg ,  DECKER, Nicole ,  KIRCHNER, Nicole ,  REINECKE, Liv

IPC分类号： C12P13/04 ,  C12N9/10 ,  C12N9/20 ,  C12N9/00

CPC分类号： C12P13/04 ,  C12N9/1025 ,  C12N9/1029 ,  C12N9/20 ,  C12N9/93 ,  C12P13/02 ,  C12Y602/01003

摘要： There is provided a microbial cell for producing at least one acyl amino acid, wherein the cell is genetically modified to comprise: - a first genetic mutation that increases the expression relative to a wild type cell of at least one lipase (EC 3.1.1) (E 1 ) capable of hydrolysing at least one glyceride to at least one fatty acid; and - a second and a third genetic mutation that increases the expression relative to a wild type cell of: (i) an amino acid-N-acyl-transferase (EC 2.3.2) (E 2 ), and (ii) an acyl-CoA synthetase (EC 6.2.1.1) (E 3 ) respectively that enables the cell to convert the fatty acid to at least one acyl amino acid and wherein the cell has a reduced fatty acid degradation capacity.

公开(公告)号：EP3008194A2

公开(公告)日：2016-04-20

申请号：EP14727495.5

申请日：2014-05-30

申请人： Evonik Degussa GmbH

发明人： WITTMANN, Eva Maria ,  HAAS, Thomas ,  SCHAFFER, Steffen ,  PÖTTER, Markus ,  SCHIEMANN, Yvonne ,  KIRCHNER, Nicole

IPC分类号： C12P5/02 ,  C12P7/04 ,  C12P7/16 ,  C12P7/30 ,  C12P7/42 ,  C12P7/52 ,  C12R1/01

CPC分类号： C12P7/52 ,  C12N15/52 ,  C12P5/026 ,  C12P7/04 ,  C12P7/16 ,  C12P7/30 ,  C12P7/42 ,  C12Y203/01009 ,  Y02E50/10 ,  Y02E50/343

摘要： The invention relates to a method for producing organic compositions comprising the method steps: A) providing an oxyhydrogen bacterium having an activity of an enzyme E
1 , which is increased by comparison with the wild type thereof and which can catalyse the conversion of 2 acetyl-CoA to acetoacetyl-CoA and CoA, in an aqueous medium; B) bringing the aqueous medium into contact with a gas containing H
2 , CO
2 and O
2 in a weight ratio from 20 - 70 to 10 - 45 to 5 - 35 and optionally C) purifying the organic composition.