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    OXIDATION UND AMINIERUNG VON PRIMÄREN ALKOHOLEN
    3.
    发明公开
    OXIDATION UND AMINIERUNG VON PRIMÄREN ALKOHOLEN 有权
    氧化铝在AMINIERUNG VONPRIMÄRENALKOHOLEN

    公开(公告)号：EP2734631A1

    公开(公告)日：2014-05-28

    申请号：EP12735553.5

    申请日：2012-07-17

    申请人： Evonik Degussa GmbH

    发明人： PÖTTER, Markus ,  HAAS, Thomas ,  PFEFFER, Jan, Christoph ,  SKERRA, Arne ,  KROUTIL, Wolfgang ,  LERCHNER, Alexandra ,  SATTLER, Johann, H. ,  SCHAFFER, Steffen ,  TAUBER, Katharina, Christin

    IPC分类号： C12P13/02

    CPC分类号： C12P13/001 ,  Y02P20/582

    摘要： The invention relates to a method comprising the following steps: a) providing a primary alcohol of the formula HO-(CH
    2 )
    x -R
    1 , in which R
    1 is selected from the group comprising -OH, -SH, -NH
    2 , and -COOR
    2 , x is at least 3, and R
    2 is selected from the group comprising H, alkyl, and aryl, b) oxidising the primary alcohol by bringing it into contact with an NAD(P)
    + -dependent alcohol dehydrogenase, and c) bringing the oxidation product from step a) into contact with a transaminase, the NAD(P)
    + -alcohol dehydrogenase and/or the transaminase being a recombinant or an isolated enzyme. The invention also relates to a whole-cell catalyst for carrying out said method, and to the use of such a whole-cell catalyst for oxidising a primary alcohol.

    摘要翻译： 一种方法，包括步骤a）提供式HO-（CH 2）x -R 1的伯醇，其中R 1是-OH，-SH，-NH 2或-COOR 2; x至少为3; 并且R2是H，烷基或芳基，b）通过使其与NAD（P）+依赖性醇脱氢酶接触来氧化伯醇，和c）使步骤a）的氧化产物与转氨酶接触，其中NAD ）+ - 醇脱氢酶和/或转氨酶是重组或分离的酶。 一种用于实施该方法的全细胞催化剂。 使用这种全细胞催化剂来氧化伯醇。

    BIOTECHNOLOGISCHE HERSTELLUNG VON 3-HYDROXYISOBUTTERSÄURE
    5.
    发明公开
    BIOTECHNOLOGISCHE HERSTELLUNG VON 3-HYDROXYISOBUTTERSÄURE 审中-公开
    生物技术制造3-羟基

    公开(公告)号：EP2788494A1

    公开(公告)日：2014-10-15

    申请号：EP12784273.0

    申请日：2012-11-14

    申请人： Evonik Degussa GmbH

    发明人： HAAS, Thomas ,  SCHAFFER, Steffen ,  PÖTTER, Markus ,  WESSEL, Mirja ,  PFEFFER, Jan, Christoph ,  GEHRING, Christian ,  KIRCHNER, Nicole ,  Wittmann, Eva, Maria

    IPC分类号： C12P7/42 ,  C12N15/52 ,  C12N9/02

    CPC分类号： C12P7/42 ,  C12N9/0004 ,  C12N9/001 ,  C12N9/1217 ,  C12N9/13 ,  C12N9/88 ,  C12N9/93 ,  C12N15/52 ,  C12Y103/99002 ,  C12Y207/02007 ,  C12Y402/01017 ,  C12Y602/01002

    摘要： The invention relates to a method having the steps of a) providing isobutyric acid, b) bringing the isobutyric acid into contact with the combination of isobutyrate kinase and phosphotransisobutyrylase and/or isobutyryl-coenzyme A-synthetase/ligase and/or isobutyrate-coenzyme A-transferase, c) bringing the product of step a) into contact with isobutyryl-coenzyme A-dehydrogenase, d) bringing the product of step b) into contact with methacrylyl-coenzyme A-hydratase, and e) hydrolyzing the product of step d) with the formation of 3-hydroxyisobutyric acid. At least one of the enzymes is used in the form of a cell which comprises an activity of 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, said activity being reduced with respect to the wild type of the cell. The invention also relates to a cell which comprises at least one enzyme of the group comprising isobutyryl-coenzyme A-synthetase/ligase, isobutyrate-coenzyme A-transferase, isobutyrate-kinase, phosphotransisobutyrylase, isobutyryl-coenzyme A-dehydrogenase, methacrylyl-coenzyme A-hydratase, and 3-hydroxisobutyryl-coenzyme A-hydrolase and which has an activity of a 3-hydroxyisobutyric acid dehydrogenase or a variant thereof, said activity being reduced with respect to the wild type of the cell. Additionally, the cell preferably has a monooxygenase, even more preferably a monooxygenase of the AlkBGT type or a variant thereof. The invention also relates to the use of such a cell for producing 3-hydroxyisobutyric acid