摘要:
The invention relates to a device and method for determining a property of a sample that is to be used in a charged particle microscope. The sample comprises a specimen embedded within a matrix layer. The device comprises a light source arranged for directing a beam of light towards said sample, and a detector arranged for detecting light emitted from said sample in response to said beam of light being incident on said sample. Finally, the device comprises a controller that is connected to said detector and arranged for determining a property of said matrix layer based on signals received by said detector.
摘要:
A sample inspection device 200 includes at least a chamber 231 formed between a top electron transparent layer 217 and a bottom electron transparent layer 218 for holding a sample. Multiple pillars 212 are arranged within the chamber. The sample inspection device includes a window 201 covering at least one of the multiple pillars 212. Various methods and devices are provided for searching the optimum sample condition of a sample for cryogenic electron microscopy. Multiple samples with different sample conditions may be screened using a sample inspection device having an additional, second, chamber formed between the top electron transparent layer and the bottom electron transparent layer.
摘要:
A method of performing Electron Energy-Loss Spectroscopy (EELS) in an electron microscope, comprising: - Providing a specimen on a specimen holder; - Producing a beam of electrons from a source; - Using an illuminator to direct said beam so as to irradiate the specimen; - Using an imaging system to receive a flux of electrons transmitted through the specimen and direct it onto a spectroscopic apparatus comprising: ▪ A dispersion device, for dispersing said flux in a dispersion direction so as to form an EELS spectrum; ▪ A detector, comprising a detection surface that is sub-divided into a plurality of detection zones,
specifically comprising: - Using a first of said detection zones to register a first EELS spectral entity; - Using a second of said detection zones to register a second EELS spectral entity; - Reading out said first detection zone whilst said second detection zone is still registering said second spectral entity.
摘要:
A method for determining a reconstructed image using a particle-optical apparatus is described. The method comprises receiving (60) the plurality of pixel signals, determining (70) a reconstructed image from using Viterbi Detection on the plurality of pixel signals, the Viterbi Detection using a plurality of different states corresponding to a plurality of configurations of particles incident on the detector, and at least two states of the plurality of different states corresponding to a same, non-zero multiplicity of incident particles on a single pixel of the plurality of pixel signals.
摘要:
The invention relates to a method and an apparatus for preparing a cryogenic sample, whereby the sample is subjected to rapid cooling using a cryogen. The method comprises the step of providing a sample, said sample comprising a specimen provided on a substantially planar specimen carrier. The method comprises the step of providing at least one flow device for transporting cryogenic fluid to said sample, wherein said flow device comprises a first nozzle for directing a flow of cryogenic fluid onto said sample. Then, the sample is positioned next to said first nozzle and a flow of cryogenic fluid is provided out of said first nozzle in such a way that the sample is cryogenically cooled. As defined herein, the nozzle opening of the first nozzle has a width, as measured in a first direction, and a height, as measured in a second direction substantially perpendicular to said first direction, wherein said width is larger than said height. Using a non-circular, such as essentially oval or rectangular nozzle opening provides for more improved cooling, so that all parts of the specimen carrier are evenly cooled.
摘要:
The invention relates to a method and an apparatus for preparing a cryogenic sample, whereby the sample is subjected to rapid cooling using a cryogen. A pair of conduits for transporting cryogenic fluid are provided, each of which conduits opens out into a mouthpiece, which mouthpieces are arranged to face each other across an intervening gap, wherein in said gap a sample that is provided on a substantially planar sample carrier can be received. Cryogenic fluid can be pumped through said conduits so as to concurrently flush from said mouthpieces and suddenly immerse the sample in cryogenic fluid from two opposite sides. As defined herein, at least one of said mouthpieces comprises at least two nozzle openings for evenly cooling said substantially planar sample carrier during said flushing.
摘要:
Apertures (108) having references edges are situated to define a sample irradiation zone (131) and a shielded zone. The sample irradiation zone (131) includes a portion proximate the shielded zone that is conjugate to a detector (116). A sample (112) is scanned into the sample irradiation zone (131) from the shielded zone so that the sample (112) can remain unexposed until situated properly with respect to the detector (116) for imaging. Irradiation exposure of the sample (112) is reduced, permitting superior imaging and less damage to the sample (112). The sample can be translated longitudinally and rotated and an electron beam (104) can be used in order to form images by electron beam tomography.
摘要:
A method of using a Transmission Charged Particle Microscope, comprising: - Providing a specimen on a specimen holder; - Using a charged particle beam column to produce a charged particle beam and irradiate at least a portion of the specimen therewith; - Using an imaging system to collect charged particles that traverse the specimen during said irradiation, and to direct them onto a detector; - Using said detector to record a diffraction pattern of said irradiated portion of the specimen, further comprising: - Configuring said detector to operate in particle counting mode; - Recording said diffraction pattern iteratively in a series of successive detection frames, and summing said frames; - During recording of each frame, causing relative motion of said diffraction pattern and said detector, so as to cause each local intensity maximum in said pattern to trace out a known, controllable locus on said detector.
摘要:
The present invention describes a blotting material comprising a profiled region that has a portion configured to protrude at least partially into a space created within a boundary formed by the rim of a holder for a sample grid. Also described are methods for making such profiles and components used to make said profiles. There are also provided methods for removing excess liquid from a sample grid by bringing the profiled blotting material into association with the sample grid past the sample grid holder. Systems comprises means to hold a sample grid holder, means to hold the blotting paper, and means to bring the two together are also described.