公开(公告)号：EP2361311B1

公开(公告)日：2017-01-18

申请号：EP09828362.5

申请日：2009-11-23

申请人： Lallemand Hungary Liquidity Management LLC ,  Stellenbosch University

发明人： MCBRIDE, John ,  BREVNOVA, Elena ,  GHANDI, Chhayal ,  MELLON, Mark ,  FROEHLICH, Alan ,  DELEAULT, Kristen ,  RAJGARHIA, Vineet ,  FLATT, Jim ,  VAN ZYL, Emile ,  DEN HAAN, Riaan ,  LAGRANGE, Danie ,  ROSE, Shaunita ,  PENTTILA, Merja ,  ILMEN, Marja ,  SIIKA-AHO, Matti ,  UUSITALO, Jaana ,  HAU, Heidi Hanson ,  RICE, Charles ,  VILLARI, Jeff ,  STONEHOUSE, Emily A. ,  GILBERT, Alan ,  KEATING, Jeffrey D. ,  XU, Haowen ,  WILLES, Deidre ,  SHIKHARE, Indraneel ,  THORNGREN, Naomi ,  WARNER, Anne K. ,  MURPHY, Dan

IPC分类号： C12N9/42 ,  C12N15/52 ,  C12P7/06

CPC分类号： C12P7/10 ,  C12N9/2437 ,  C12N9/2445 ,  C12N15/80 ,  C12P7/14 ,  C12P39/00 ,  C12Y302/01004 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/16 ,  Y02E50/17

公开(公告)号：EP3168300A1

公开(公告)日：2017-05-17

申请号：EP16192843.7

申请日：2011-06-03

申请人： Lallemand Hungary Liquidity Management LLC ,  Stellenbosch University

发明人： BREVNOVA, Elena ,  MCBRIDE, John, E. ,  WISWALL, Erin ,  WENGER, Kevin, S. ,  CAIAZZA, Nicky ,  HAU, Heidi, H. ,  ARGYROS, Aaron ,  AGBOGBO, Frank ,  RICE, Charles, F. ,  BARRETT, Trisha ,  BARDSLEY, John, S. ,  FOSTER, Abigail, S. ,  WARNER, Anne, K. ,  MELLON, Mark ,  SKINNER, Ryan ,  SHIKHARE, Indraneel ,  DEN HAAN, Riaan ,  GANDHI, Chhayal, V. ,  BELCHER, Alan ,  RAJGARHIA, Vineet, B. ,  FROEHLICH, Allan, C. ,  DELEAULT, Kristen, M. ,  STONEHOUSE, Emily ,  TRIPATHI, Shital, A. ,  GOSSELIN, Jennifer ,  CHIU, Yin-Ying ,  XU, Haowen

IPC分类号： C12N9/42 ,  C12P7/06

CPC分类号： C12N15/52 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/248 ,  C12N9/2482 ,  C12N15/81 ,  C12P7/06 ,  C12Y302/01004 ,  C12Y302/01008 ,  C12Y302/01021 ,  C12Y302/01091 ,  Y02E50/17

摘要： The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured togetherand used to produce ethanol from biomass feedstock.

摘要翻译： 本发明涉及一种或多种酵母菌株，其分泌全套或全套的任何子集的酶以水解玉米淀粉，玉米纤维，木质纤维素（包括水解纤维素，半纤维素和 在木质素和碳水化合物之间）并利用戊糖（木糖和阿拉伯糖）。 本发明还涉及用于每种酶活性类别的在酵母中良好表达的一组蛋白质。 所得菌株或菌株可用于同时水解淀粉和纤维素。 得到的菌株或菌株也可以被代谢工程化以产生更少的甘油并摄取乙酸盐。 所得到的菌株或菌株也可用于从粒状淀粉生产乙醇而不液化。 得到的一种或多种菌株可以进一步用于减少在同时糖化和发酵（SSF）过程中水解生物质原料所需的外部酶的量，或者在当前的糖分解酶载量下在SSF期间增加乙醇的产量。 另外，本发明的多种酶可以在本发明的细胞中共表达以提供对生物质原料的协同消化作用。 在一些方面，表达不同异源糖分解酶的宿主细胞也可以共培养以用于从生物质原料生产乙醇。

公开(公告)号：EP2890784A1

公开(公告)日：2015-07-08

申请号：EP13719189.6

申请日：2013-03-15

申请人： Lallemand Hungary Liquidity Management LLC

发明人： MCBRIDE, John, E. ,  WISWALL, Erin ,  SHIKHARE, Indraneel ,  XU, Haowen ,  THORNGREN, Naomi ,  HAU, Heidi, H. ,  STONEHOUSE, Emily

IPC分类号： C12N9/24 ,  C12N15/81

CPC分类号： C12N9/18 ,  C12N9/2402 ,  C12N9/2437 ,  C12N9/2445 ,  C12N9/2465 ,  C12N9/2471 ,  C12N9/248 ,  C12N9/2482 ,  C12N9/2491 ,  C12N9/2494 ,  C12P3/00 ,  C12P7/10 ,  C12P7/16 ,  C12P7/28 ,  C12P7/52 ,  C12P7/56 ,  C12P19/02 ,  C12P19/14 ,  C12Y301/01006 ,  C12Y301/01072 ,  C12Y302/01021 ,  C12Y302/01037 ,  C12Y302/01072 ,  C12Y302/01131 ,  C12Y302/01139 ,  Y02E50/16

摘要： The present invention provides a multi-component enzyme system that hydrolyzes hemicellulose oligomers from hardwood which can be expressed, for example, in yeast such as Saccharomyces cerevisiae. In some embodiments, this invention provides for the engineering of a series of biocatalysts combining the expression and secretion of components of this enzymatic system with robust, rapid xylose utilization, and ethanol fermentation under industrially relevant process conditions for consolidated bioprocessing. In some embodiments, the invention utilizes co-cultures of strains that can achieve significantly improved performance due to the incorporation of additional enzymes in the fermentation system.

摘要翻译： 本发明提供了一种多组分酶系统，其从硬木中水解半纤维素寡聚体，其可以在例如酿酒酵母（Saccharomyces cerevisiae）的酵母中表达。 在一些实施方案中，本发明提供了一系列生物催化剂的工程化，其将该酶系统的组分的表达和分泌与强力，快速的木糖利用和乙醇发酵相结合，在工业上相关的工艺条件下进行综合生物处理。 在一些实施方案中，本发明利用菌株的共培养物，其可以通过在发酵系统中引入额外的酶而实现显着改善的性能。