公开(公告)号：EP3215271A4

公开(公告)日：2018-06-13

申请号：EP15856708

申请日：2015-11-03

申请人： MASSACHUSETTS GEN HOSPITAL

发明人： KAPUR RAVI ,  SMITH KYLE C ,  TONER MEHMET

IPC分类号： G01N35/08 ,  A61K35/28 ,  B01L3/00 ,  C12M1/12 ,  C12N5/0787 ,  G01N15/00 ,  G01N15/02 ,  G01N15/06

CPC分类号： B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493

摘要： A microfluidic device includes a particle sorting region having a first, second and third microfluidic channels, a first array of islands separating the first microfluidic channel from the second microfluidic channel, and a second array of islands separating the first microfluidic channel from the third microfluidic channel, in which the island arrays and the microfluidic channels are arranged so that a first fluid is extracted from the first microfluidic channel into the second microfluidic channel and a second fluid is extracted from the third microfluidic channel into the first microfluidic channel, and so that particles are transferred from the first fluid sample into the second fluid sample within the first microfluidic channel.

公开(公告)号：EP3215270A4

公开(公告)日：2018-05-23

申请号：EP15856423

申请日：2015-11-03

申请人： MASSACHUSETTS GEN HOSPITAL

发明人： KAPUR RAVI ,  SMITH KYLE C ,  TONER MEHMET

IPC分类号： B01L3/00 ,  A61K35/28 ,  B81B1/00 ,  G01N15/00 ,  G01N15/02 ,  G01N15/06 ,  G01N35/08

CPC分类号： B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493

摘要： Extracting and concentrating particles from a first fluid sample includes: providing the first fluid sample to a fluid exchange module of a microfluidic device, providing a second fluid sample to the fluid exchange module, in which the first fluid sample and the second fluid sample are provided under conditions such that particle-free portions of the first fluid sample are shifted, and an inertial lift force causes the particles in the first fluid sample to cross streamlines and transfer into the second fluid sample; passing the second fluid sample containing the transferred particles to a particle concentration module under conditions such that particle-free portions of the second fluid sample are shifted, and such that the particles within the second fluid sample are focused to a streamline within the particle concentration module.

公开(公告)号：EP3215854A4

公开(公告)日：2018-05-23

申请号：EP15856773

申请日：2015-11-03

申请人： MASSACHUSETTS GEN HOSPITAL

发明人： KAPUR RAVI ,  SMITH KYLE C ,  TONER MEMHET

IPC分类号： G01N35/08 ,  A61K35/28 ,  B01L3/00 ,  C12M1/12 ,  C12N5/0787 ,  G01N15/00 ,  G01N15/02 ,  G01N15/06

CPC分类号： B01L3/502761 ,  A61K35/28 ,  B01L3/502715 ,  B01L3/502746 ,  B01L3/502753 ,  B01L3/502776 ,  B01L2200/0631 ,  B01L2200/0647 ,  B01L2200/0652 ,  B01L2200/0668 ,  B01L2200/0684 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2300/0877 ,  B01L2300/185 ,  B01L2400/0409 ,  B01L2400/0415 ,  B01L2400/043 ,  B01L2400/0436 ,  B01L2400/0457 ,  B01L2400/0487 ,  B01L2400/082 ,  G01N1/4077 ,  G01N15/0255 ,  G01N15/0618 ,  G01N15/1484 ,  G01N2001/4088 ,  G01N2015/0053 ,  G01N2015/0065 ,  G01N2015/0288 ,  G01N2015/1486 ,  G01N2015/149 ,  G01N2015/1493

摘要： Extracting and concentrating particles from a first fluid sample includes: providing the first fluid sample to a fluid exchange module of a microfluidic device, providing a second fluid sample to the fluid exchange module, in which the first fluid sample and the second fluid sample are provided under conditions such that particle-free portions of the first fluid sample are shifted, and an inertial lift force causes the particles in the first fluid sample to cross streamlines and transfer into the second fluid sample; passing the second fluid sample containing the transferred particles to a particle concentration module under conditions such that particle-free portions of the second fluid sample are shifted, and such that the particles within the second fluid sample are focused to a streamline within the particle concentration module.

公开(公告)号：EP3058316A4

公开(公告)日：2017-05-31

申请号：EP14853592

申请日：2014-10-20

申请人： MASSACHUSETTS GEN HOSPITAL

发明人： SPUHLER PHILIPP S ,  SMITH KYLE C ,  FACHIN FABIO ,  BARBER THOMAS ALAN ,  KAPUR RAVI ,  TONER MEHMET ,  PAI VINCENT ,  KARABACAK MURAT N

IPC分类号： B01L3/00 ,  B03C1/01 ,  B03C1/033 ,  B03C1/28 ,  G01N15/10 ,  G01N15/14 ,  G01N35/00 ,  G01N35/10

CPC分类号： C12M47/04 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0652 ,  B01L2300/0816 ,  B01L2300/0864 ,  B01L2400/043 ,  B01L2400/0487 ,  B01L2400/086 ,  B03C1/01 ,  B03C1/0332 ,  B03C1/288 ,  B03C2201/18 ,  B03C2201/26 ,  C12M23/16 ,  G01N15/1463 ,  G01N35/0098 ,  G01N2015/1006 ,  G01N2015/149 ,  G01N2035/00237 ,  G01N2035/1034

摘要： Microfluidic devices are described that include a microfluidic channel, a first array of one or more magnets above the microfluidic channel, each magnet in the first array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the first array, and a second array of one or more magnets beneath the microfluidic channel, each magnet in the second array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the second array. The first array is aligned with respect to the second array such that magnetic fields emitted by the first array and second array generate a magnetic flux gradient profile extending through the channel. An absolute value of the profile includes a first maximum and a second maximum that bound a local minimum. The local minimum is located within the microfluidic channel or less than 5 mm away from a wall of the microfluidic channel. Methods of using the new devices are also described.

摘要翻译： 描述了微流体装置，其包括微流体通道，微流体通道上方的一个或多个磁体的第一阵列，第一阵列中的每个磁体具有与第一阵列中的相邻磁体的磁极方向相反的磁极方向， 在微流体通道下方的一个或多个磁体的第二阵列，第二阵列中的每个磁体具有与第二阵列中的相邻磁体的磁极取向相反的磁极取向。 第一阵列相对于第二阵列对齐，使得由第一阵列和第二阵列发射的磁场产生延伸通过通道的磁通梯度分布。 概况的绝对值包括限制局部最小值的第一最大值和第二最大值。 局部最小值位于微流体通道内或离微流体通道壁不到5mm。 还描述了使用新设备的方法。