公开(公告)号：EP1183346B1

公开(公告)日：2007-10-31

申请号：EP00932712.3

申请日：2000-05-23

申请人： NEW ENGLAND BIOLABS, INC. ,  Boston Biomedical Research Institute

发明人： XU, Ming-Qun ,  EVANS, Thomas, C. ,  PRADHAN, Sriharsa ,  COMB, Donald, G. ,  PAULUS, Henry ,  SUN, Luo ,  CHEN, Lixin ,  GHOSH, Inca

IPC分类号： C12N15/10 ,  C12N15/62 ,  C12N15/64 ,  C12P21/02

CPC分类号： C12N9/1029 ,  C07K14/43595 ,  C07K2319/00 ,  C07K2319/055 ,  C07K2319/06 ,  C07K2319/08 ,  C07K2319/09 ,  C07K2319/32 ,  C07K2319/75 ,  C07K2319/92 ,  C12N9/1092 ,  C12N9/1252 ,  C12N9/88 ,  C12N15/10 ,  C12N15/62 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8257 ,  C12N15/8275 ,  C12N15/8278 ,  C12P21/02

摘要： A new type of transgene system is disclosed which allows efficient protein expression in a target host such as a plant, but avoids the undesirable result of the migration of the transgene into related host systems and/or to the environment via the pollen. The methods described herein may also be applied to the expression of virtually any protein of interest (e.g. a toxic protein) in eukaryotic (yeast, insect, mammalian cells, etc.) and prokaryotic (E. coli, etc.) organisms.

公开(公告)号：EP1183346A1

公开(公告)日：2002-03-06

申请号：EP00932712.3

申请日：2000-05-23

申请人： NEW ENGLAND BIOLABS, INC. ,  Boston Biomedical Research Institute

发明人： XU, Ming-Qun ,  EVANS, Thomas, C. ,  PRADHAN, Sriharsa ,  COMB, Donald, G. ,  PAULUS, Henry ,  SUN, Luo ,  CHEN, Lixin ,  GHOSH, Inca

IPC分类号： C12N15/10 ,  C12N15/62 ,  C12N15/64 ,  C12P21/02

CPC分类号： C12N9/1029 ,  C07K14/43595 ,  C07K2319/00 ,  C07K2319/055 ,  C07K2319/06 ,  C07K2319/08 ,  C07K2319/09 ,  C07K2319/32 ,  C07K2319/75 ,  C07K2319/92 ,  C12N9/1092 ,  C12N9/1252 ,  C12N9/88 ,  C12N15/10 ,  C12N15/62 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8257 ,  C12N15/8275 ,  C12N15/8278 ,  C12P21/02

摘要： A new type of transgene system is disclosed which allows efficient protein expression in a target host such as a plant, but avoids the undesirable result of the migration of the transgene into related host systems and/or to the environment via the pollen. The methods described herein may also be applied to the expression of virtually any protein of interest (e.g. a toxic protein) in eukaryotic (yeast, insect, mammalian cells, etc.) and prokaryotic (E. coli, etc.) organisms.

公开(公告)号：EP1578962A2

公开(公告)日：2005-09-28

申请号：EP03812946.6

申请日：2003-12-11

申请人： NEW ENGLAND BIOLABS, INC.

发明人： XU, Ming-Qun ,  SUN, Luo ,  GHOSH, Inca ,  EVANS, Thomas, C.

IPC分类号： C12N9/00

CPC分类号： G01N33/54353 ,  G01N33/532

摘要： Improved methods for purification, detection or characterization of molecules have been described in embodiments of the present invention. The improved methods rely on the ability to create a carrier reagent which is capable of (i) binding to a matrix either specifically or non-specifically; and (ii) forming a covalent linkage with any ligand having a nucleophilic group or a thioester as a result of a simple reaction which does not require a variety of chemical reagents or sophisticated chemistry. The covalent linkage between the carrier and the ligand relies on the chemical reaction between a thioester and a nucleophilic group. The carrier should either contain a reactive thioester which if it is a protein should be at the C-terminal end of the protein or a reactive nucleophilic group which if it is a protein should be at the N-terminal end and should preferably be a cysteine or selenocysteine. The ligand requires a reactive nucleophilic group to react with the thioester on the carrier or if the carrier has a reactive nucleophilic group, the ligand should have a reactive thioester.