公开(公告)号：EP1183346B1

公开(公告)日：2007-10-31

申请号：EP00932712.3

申请日：2000-05-23

申请人： NEW ENGLAND BIOLABS, INC. ,  Boston Biomedical Research Institute

发明人： XU, Ming-Qun ,  EVANS, Thomas, C. ,  PRADHAN, Sriharsa ,  COMB, Donald, G. ,  PAULUS, Henry ,  SUN, Luo ,  CHEN, Lixin ,  GHOSH, Inca

IPC分类号： C12N15/10 ,  C12N15/62 ,  C12N15/64 ,  C12P21/02

CPC分类号： C12N9/1029 ,  C07K14/43595 ,  C07K2319/00 ,  C07K2319/055 ,  C07K2319/06 ,  C07K2319/08 ,  C07K2319/09 ,  C07K2319/32 ,  C07K2319/75 ,  C07K2319/92 ,  C12N9/1092 ,  C12N9/1252 ,  C12N9/88 ,  C12N15/10 ,  C12N15/62 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8257 ,  C12N15/8275 ,  C12N15/8278 ,  C12P21/02

摘要： A new type of transgene system is disclosed which allows efficient protein expression in a target host such as a plant, but avoids the undesirable result of the migration of the transgene into related host systems and/or to the environment via the pollen. The methods described herein may also be applied to the expression of virtually any protein of interest (e.g. a toxic protein) in eukaryotic (yeast, insect, mammalian cells, etc.) and prokaryotic (E. coli, etc.) organisms.

公开(公告)号：EP1183346A1

公开(公告)日：2002-03-06

申请号：EP00932712.3

申请日：2000-05-23

申请人： NEW ENGLAND BIOLABS, INC. ,  Boston Biomedical Research Institute

发明人： XU, Ming-Qun ,  EVANS, Thomas, C. ,  PRADHAN, Sriharsa ,  COMB, Donald, G. ,  PAULUS, Henry ,  SUN, Luo ,  CHEN, Lixin ,  GHOSH, Inca

IPC分类号： C12N15/10 ,  C12N15/62 ,  C12N15/64 ,  C12P21/02

CPC分类号： C12N9/1029 ,  C07K14/43595 ,  C07K2319/00 ,  C07K2319/055 ,  C07K2319/06 ,  C07K2319/08 ,  C07K2319/09 ,  C07K2319/32 ,  C07K2319/75 ,  C07K2319/92 ,  C12N9/1092 ,  C12N9/1252 ,  C12N9/88 ,  C12N15/10 ,  C12N15/62 ,  C12N15/8214 ,  C12N15/8216 ,  C12N15/8241 ,  C12N15/8242 ,  C12N15/8257 ,  C12N15/8275 ,  C12N15/8278 ,  C12P21/02

摘要： A new type of transgene system is disclosed which allows efficient protein expression in a target host such as a plant, but avoids the undesirable result of the migration of the transgene into related host systems and/or to the environment via the pollen. The methods described herein may also be applied to the expression of virtually any protein of interest (e.g. a toxic protein) in eukaryotic (yeast, insect, mammalian cells, etc.) and prokaryotic (E. coli, etc.) organisms.

公开(公告)号：EP0835323A1

公开(公告)日：1998-04-15

申请号：EP96922498.0

申请日：1996-06-19

申请人： NEW ENGLAND BIOLABS, INC.

发明人： COMB, Donald, G. ,  PERLER, Francine, B. ,  JACK, William, E. ,  XU, Ming-Qun ,  HODGES, Robert, A. ,  NOREN, Christopher, J. ,  CHONG, Shaorong, S., C.

IPC分类号： C12N15 ,  C07K1 ,  C07K14 ,  C07K19 ,  C12N9 ,  C12P21

CPC分类号： C12Y302/01081 ,  C07K1/1075 ,  C07K2319/20 ,  C07K2319/21 ,  C07K2319/92 ,  C07K2319/95 ,  C12N9/1252 ,  C12N9/14 ,  C12N9/22 ,  C12N9/2442 ,  C12N9/2471 ,  C12N15/62 ,  C12P21/02 ,  C12Y302/01014 ,  C12Y302/01023

摘要： The present invention is directed to modified proteins and methods of their production. The modified proteins comprise a controllable intervening protein sequence (CIVPS) inserted into a target protein, the CIVPS being capable of excision from or cleavage of the modified protein under predetermined conditions in cis or in trans, i.e., increase in temperature, exposure to light, unblocking of amino acid residues by dephosphorylation, treatment with chemical reagents or deglycosylation. If desired, the modified protein can be subjected to these conditions. The CIVPS may also be inserted into a region that substantially inactivates target protein activity.