摘要:
[PROBLEM] To provide means for efficient growth of epithelial cells capable of being used to manufacture regenerated hair follicle germs. [SOLUTION MEANS] Provided are a culture medium for growth of epithelial cells capable of being used to manufacture regenerated hair follicle germs, the culture medium comprising basal medium and at least (1) through (3) below; and a method for growing the epithelial cells using the culture medium: (1) at least one species of BMP inhibitor; (2) at least one species of fibroblast growth factor; and (3) at least one species of sonic hedgehog (SHH) and/or SHH agonist.
摘要:
The present invention provides a method for producing regenerated hair follicle primordium that allows production of regenerated hair follicle primordium more easily and in larger amounts compared to conventional production methods. A method for producing regenerated hair follicle primordium comprising a step of obtaining hair follicle primordium by culturing a first population of cells comprising epithelial cells and a second population of cells comprising mesenchymal cells while allowing them to be in contact is provided. The production method of the present invention is characterized in that at least either one of said first population of cells and said second population of cells has formed cell aggregates before said contact, and a culture support is not used when contacting the other population of cells with said cell aggregate.
摘要:
The purpose of the present invention is to propose a method for measuring the level of health of a subject, with which it is possible: to perform health examination that is non-invasive to the human body and that employs a simple method; to provide highly reliable analysis results having a scientific basis; and to, additionally, provide appropriate solutions to the subject. Another purpose of the present invention is to propose a health-level determining apparatus employing said method and a new business model employing said method. The present invention is a method for measuring the level of health of a subject, including a hair analyzing step (A) for analyzing hair of the subject. It is preferable that the aforementioned hair analyzing step (A) include a step (A-1) for analyzing hair in the occipital region. In addition, it is preferable that the aforementioned hair analyzing step (A) include a step (A-2) for analyzing hair in a site other than the occipital region.
摘要:
The object of the present invention is to provide an artificial skin having hair follicles, sebaceous glands, and hair pores. The present invention provides a method for manufacturing artificial skin having hair follicles, sebaceous glands, and hair pores, characterized in that it comprises each of the following steps: (A) : a step of preparing an artificial skin having dermal and epidermal layers or an artificial skin having only a dermal layer; and (B): a step of transplanting isolated hair follicles to the artificial skin prepared in step (A); wherein said isolated hair follicle comprises a sebaceous gland, and said isolated hair follicle is transplanted to said artificial skin so that the surface of said dermal layer of said artificial skin is in alignment with the position of the hair pore portion of said isolated hair follicle.
摘要:
[Problem] To provide a technique for regenerating hair through the transplantation of hair follicle germ in which the color of the regenerated hair is controlled. [Solution] Provided is a method for producing a regenerative hair follicle germ for transplantation in which a color of hair that grows after transplantation is controlled, said method including: preparing a first cell mass including mesenchymal cells; preparing a second cell mass including epithelial cells; preparing a cell mass including pigment stem cells; binding the cell mass including the pigment stem cells to at least one among the first cell mass and the second cell mass, and closely contacting the first cell mass and the second cell mass, at least one of which has been bound to the cell mass including the pigment stem cells, and culturing them within a support.
摘要:
The present invention provides a method for efficiently manufacturing full-thickness skin with skin appendage. The present invention provides a method for manufacturing full-thickness skin with skin appendage, characterized in that said method comprises the following steps (a) - (d): (a) a step of stimulating an embryoid body with a bioactive substance that may activate the Wnt pathway, (b) a step of preparing a conjugate comprising all or a part of said embryoid body stimulated in step (a) and a scaffolding material, (c) a step of transplanting said conjugate prepared in said step (b) to an animal, and (d) a step of manufacturing full-thickness skin derived from said conjugate in said animal.
摘要:
The purpose of the present invention is to provide a method for producing a regenerative organ primordium for transplantation, said regenerative organ primordium for transplantation ensuring continuity with a recipient after transplantation and facilitating transplantation procedures. Provided is a method for producing a regenerative organ primordium provided with a guide for transplantation, said method comprising: a step for preparing a regenerative organ primordium by tightly contacting a first cell mass, which substantially consists of mesenchymal cells, with a second cell mass, which substantially consists of epithelial cells, and culturing these cell masses within a supporting medium; and a step for inserting the guide into the regenerative organ primordium.