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公开(公告)号：EP2771457A4
公开(公告)日：2015-04-08
申请号：EP12844270
申请日：2012-10-25
发明人： COST GREGORY J , HOLMES MICHAEL C , KASAHARA NORIYUKI , LAGANIERE JOSEE , MILLER JEFFREY C , PASCHON DAVID , REBAR EDWARD J , URNOV FYODOR , ZHANG LEI
CPC分类号： C12N15/85 , C07K2319/81 , C12N9/1077 , C12N9/22 , C12N9/96 , C12N15/907 , C12Y204/02008 , C12Y301/21004
摘要： Nucleases and methods of using these nucleases for modification of an HPRT locus and for increasing the frequency of gene modification at a targeted locus and clones and for generating animals.
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2.发明公开METHODS AND COMPOSITIONS FOR GENOME ENGINEERING 审中-公开VERFAHREN UND ZUSAMMENSETZUNGENFÜRGENOM-ENGINEERING
公开(公告)号：EP3080274A4
公开(公告)日：2017-06-21
申请号：EP14869719
申请日：2014-12-09
发明人： MILLER JEFFREY C , PASCHON DAVID , REBAR EDWARD J , WECHSLER THOMAS , ZHANG LEI
CPC分类号： C07K14/47 , A61K48/00 , C07K14/4702 , C07K2319/81 , C12N9/22 , C12N9/644 , C12N15/86 , C12N2750/14133 , C12N2750/14142 , C12N2750/14143
摘要： Disclosed herein are methods and compositions for insertion of transgene sequences encoding proteins involved in clotting into the genome of a cell for treating conditions including hemophilias.
摘要翻译： 本文公开了用于插入转基因序列的方法和组合物，所述转基因序列编码涉及凝结成细胞基因组的蛋白质以治疗包括血友病的病症。
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3.发明公开COMPOSITIONS FOR LINKING DNA-BINDING DOMAINS AND CLEAVAGE DOMAINS 审中-公开ZUSAMMENSETZUNGEN ZUR VERNETZUNG VON DNA-BINDUNGSDOMÄNENUND-SPALTUNGSDOMÄNEN
公开(公告)号：EP3039136A4
公开(公告)日：2017-05-03
申请号：EP14840079
申请日：2014-08-28
发明人： PASCHON DAVID , ZHANG LEI
CPC分类号： C12N15/907 , C07K2319/81 , C12N9/22 , C12N15/01 , C12N15/62
摘要： Disclosed herein are compositions for linking DNA-binding domains and cleavage domains to form nucleases, for example nucleases with altered target site separation (gap) preferences as compared to conventional linkers. Also described are fusion proteins comprising these linkers. The disclosure also provides methods of using these fusion proteins and compositions thereof for targeted cleavage of cellular chromatin in a region of interest and/or homologous recombination at a predetermined region of interest in cells. Thus, in one aspect, described herein are amino acid sequences linking a DNA-binding domain to a cleavage domain. The linker may replace one or more residues of the DNA-binding domain and/or cleavage domain.
摘要翻译： 本文公开了用于连接DNA结合结构域和切割结构域（或切割半结构域）以形成非天然存在的核酸酶的组合物。 还描述了制造和使用包含这些连接体的组合物的方法。
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公开(公告)号：EP3102673A4
公开(公告)日：2017-11-01
申请号：EP15742584
申请日：2015-02-02
发明人： LIU PEI-QI , REIK ANDREAS , ZHANG LEI
CPC分类号： C07K14/805 , A61K35/28 , A61K2035/124
摘要： Methods and compositions for treatment of a beta thalessemia are provided.
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公开(公告)号：EP2890780A4
公开(公告)日：2016-08-31
申请号：EP13833658
申请日：2013-08-29
发明人： COST GREGORY J , GREGORY PHILIP D , GUSCHIN DMITRY , HOLMES MICHAEL C , MILLER JEFFREY C , PASCHON DAVID , REBAR EDWARD J , REIK ANDREAS , URNOV FYODOR , ZHANG LEI
IPC分类号： C12N5/071 , C07K14/47 , C07K14/805 , C12N9/22 , C12N15/85
CPC分类号： C12N15/85 , C07K14/4702 , C07K14/805 , C07K2319/81 , C12N9/22 , C12N15/52
摘要： Disclosed herein are nucleases and methods of using these nucleases for alteration of a globin gene and generation of cells.
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公开(公告)号：EP2780460A4
公开(公告)日：2015-08-26
申请号：EP12850371
申请日：2012-11-16
发明人： GREGORY PHILIP D , MILLER JEFFREY C , PASCHON DAVID , REBAR EDWARD J , TAN SIYUAN , URNOV FYODOR , ZHANG LEI
IPC分类号： C12N15/87 , C07K14/195 , C12N5/04 , C12N5/071 , C12N9/22 , C12N15/63 , C12N15/90 , C12P19/34
CPC分类号： C07K14/195 , C07K2319/80 , C12N1/16 , C12N5/04 , C12N5/0602 , C12N9/1007 , C12N9/22 , C12N15/63 , C12N15/902 , C12N15/907 , C12P19/34 , C12Y301/21004
摘要： Disclosed herein are enhanced polypeptides, polynucleotides encoding these polypeptides, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
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7.发明公开
公开(公告)号：EP2571512A4
公开(公告)日：2013-11-20
申请号：EP11783865
申请日：2011-05-17
发明人： GREGORY PHILIP D , MILLER JEFFREY C , PASCHON DAVID , REBAR EDWARD J , TAN SIYUAN , URNOV FYODOR , ZHANG LEI
CPC分类号： C12N15/907 , A61K38/00 , C07K14/195 , C07K2319/80 , C12N9/22 , C12N15/52 , C12N15/62 , C12N15/8213 , C12N15/85 , C12Y301/21004
摘要： Disclosed herein are polypeptides, polynucleotides encoding, cells and organisms comprising novel DNA-binding domains, including TALE DNA-binding domains. Also disclosed are methods of using these novel DNA-binding domains for modulation of gene expression and/or genomic editing of endogenous cellular sequences.
摘要翻译： 本文公开了包含新型DNA结合结构域的多肽，编码多核苷酸的细胞和生物体，包括TALE DNA结合结构域。 还公开了使用这些新的DNA结合结构域调节内源性细胞序列的基因表达和/或基因组编辑的方法。
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公开(公告)号：EP1720995A4
公开(公告)日：2008-04-16
申请号：EP05756438
申请日：2005-02-03
发明人： MILLER JEFFREY C , ZHANG LEI
IPC分类号： C12N9/22 , C07K14/435 , C07K14/47 , C07K14/715 , C12N9/14 , C12N15/01 , C12N15/62 , C12P19/34 , C12Q1/68
CPC分类号： A61K35/12 , C07K14/43595 , C07K14/4717 , C07K14/715 , C07K2319/00 , C07K2319/09 , C07K2319/81 , C12N9/14 , C12N9/22 , C12N15/62 , C12N15/85 , C12N2800/80
摘要： Disclosed herein are methods and compositions for targeted cleavage of a genomic sequence, targeted alteration of a genomic sequence, and targeted recombination between a genomic region and an exogenous polynucleotide homologous to the genomic region. The compositions include fusion proteins comprising a cleavage domain (or cleavage half-domain) and an engineered zinc finger domain, as well as polynucleotides encoding same. Fusion proteins comprising cleavage half-domains are used in pairs, to reconstitute a functional cleavage domain. In these fusion proteins, the zinc finger domain can be N-terminal to the cleavage half-domain, or the cleavage half-domain can be N-terminal to the zinc finger domain. The availability of fusion endonucleases having these different polarities allows targeting (and thereby binding) of zinc finger endonucleases either to opposite strands of the DNA target or to the same strand of the DNA target, thereby increasing the number of possible sequences which can be targeted and cleaved by the fusion proteins.
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9.发明公开TARGETED DISRUPTION OF T CELL RECEPTOR GENES USING ENGINEERED ZINC FINGER PROTEIN NUCLEASES 审中-公开GEZIELTE DISRUPTION VON T-ZELL REZEPTORGENEN MIT MANIPULIERTEN ZINKFINGER-PROTEINNUKLEASEN
公开(公告)号：EP2975942A4
公开(公告)日：2016-11-09
申请号：EP14770870
申请日：2014-03-20
发明人： GREGORY PHILIP D , HOLMES MICHAEL C , PASCHON DAVID , ZHANG LEI , BONINI MARIA CHIARA , GENOVESE PIETRO , MAGNANI ZULMA , MASTAGLIO SARA , NALDINI LUIGI
CPC分类号： A61K35/26 , A61K39/0011 , A61K2039/5156 , A61K2039/5158 , C12N15/87 , C12N2501/515 , C12N2510/00
摘要： Disclosed herein are methods and compositions for modifying TCR genes, using nucleases (zinc finger nucleases or TAL nucleases) to modify TCR genes.
摘要翻译： 本文公开了使用核酸酶（锌指核酸酶或TAL核酸酶）修饰TCR基因来修饰TCR基因的方法和组合物。
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10.发明公开FAD2 PERFORMANCE LOCI AND CORRESPONDING TARGET SITE SPECIFIC BINDING PROTEINS CAPABLE OF INDUCING TARGETED BREAKS 审中-公开FAD2演出场所和相关的目标定特异性结合蛋白，以确保靶向BREAKS
公开(公告)号：EP2892321A4
公开(公告)日：2016-03-02
申请号：EP13834776
申请日：2013-09-05
发明人： AINLEY WILLIAM MICHAEL , WEBB STEVEN R , SAMUEL PON , GUSCHIN DMITRY Y , MILLER JEFFREY C , ZHANG LEI
CPC分类号： C12N15/8216 , C07K14/4702 , C07K2319/81 , C12N9/22 , C12N15/102 , C12N15/66 , C12N15/822 , C12N15/8247 , C12N15/902 , C12Y301/00
摘要： Methods and compositions for gene disruption, gene editing or gene stacking within a FAD2 loci by cleaving, in a site directed manner, a location in a FAD2 gene in a soybean cell, to generate a break in the FAD2 gene and then optionally integrating into the break a nucleic acid molecule of interest is disclosed.
摘要翻译： 方法和组合物基因破坏，基因编辑或基因通过切割，在一个定点方式一个FAD2基因座内堆叠，在大豆细胞中的FAD2基因的位置，以产生在所述FAD2基因中断，然后任选INTEGRA廷入 打破感兴趣的核酸分子游离缺失盘。
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