公开(公告)号：EP1005377A2

公开(公告)日：2000-06-07

申请号：EP98943466.7

申请日：1998-08-28

申请人： Selective Genetics, Inc.

发明人： LAROCCA, David ,  BAIRD, Andrew ,  JOHNSON, Wendy

IPC分类号： A61K48/00 ,  C12N15/70

CPC分类号： C40B40/02 ,  A61K47/6901 ,  A61K48/00 ,  C07K14/005 ,  C07K14/503 ,  C07K2319/00 ,  C12N7/00 ,  C12N15/1037 ,  C12N15/86 ,  C12N15/87 ,  C12N2795/10022 ,  C12N2795/10043 ,  C12N2795/10045 ,  C12N2795/14122 ,  C12N2810/851

摘要： Filamentous phage particles displaying a ligand on their surface are used to deliver a therapeutic gene to a cell. The ligand is a fusion protein with a phage capsid protein, covalently conjugated to phage particles, or complexed with modified phage particles.

公开(公告)号：EP1009819A1

公开(公告)日：2000-06-21

申请号：EP98942312.4

申请日：1998-08-28

申请人： Selective Genetics, Inc.

发明人： LAROCCA, David

IPC分类号： C12N15/10 ,  C07K14/47 ,  G01N33/68 ,  C12Q1/68 ,  C07K14/71

CPC分类号： C40B40/02 ,  A61K47/6901 ,  C07K2319/00 ,  C12N15/1037 ,  C12N2799/021 ,  C12Q1/6811 ,  C12Q1/6897

摘要： A bacteriophage system is presented for selecting internalizing ligands for gene delivery. The bacteriophage carries a reporter or selectable marker and presents a ligand on its surface. More specifically, a library of potential ligands may be screened for the ability to successfully transduce target cells.

摘要翻译： 提出了用于选择用于基因递送的内化配体的噬菌体系统。 噬菌体携带报道基因或选择标记，并在其表面上呈递配体。 更具体地说，可以筛选潜在配体文库成功转导靶细胞的能力。

公开(公告)号：EP1402074A2

公开(公告)日：2004-03-31

申请号：EP02726900.0

申请日：2002-05-17

申请人： Selective Genetics, Inc.

发明人： LAROCCA, David ,  KASSNER, Paul ,  BAIRD, Andrew ,  BURG, Michael, Alan

IPC分类号： C12Q1/70 ,  C12P21/06 ,  C12N15/00 ,  C07H21/04

CPC分类号： C40B40/02 ,  C12N15/1037 ,  C12Q2600/158

摘要： A genetic package display system and methodology for probing protein-protein interactions that lead to cell transduction, selecting and/or identifying internalizing ligands, target cells and tissues which internalize known or putative ligands, and cell transduction facilitating peptides is provided. A ligand displaying genetic package that carries a selectable marker (e.g., reporter, selection, etc.) and presents a ligand on its surface is utilized to identify internalizing ligands, tranduction facilitating peptides, and/or a variety of cells and tissue types for the ability to be successfully transduced by the ligand displaying genetic package. Also provided are methods for evolving a ligand displaying package to facilitate gene delivery or delivery of any desired agent (e.g., pharmaceutical, polypeptide, peptide, etc.) into a cell and/or targeting cellular compartments such as the nucleus, endosome, chloroplast, mitochondria, etc.

公开(公告)号：EP1133553A1

公开(公告)日：2001-09-19

申请号：EP99956763.9

申请日：1999-10-29

申请人： Selective Genetics, Inc.

发明人： LAROCCA, David ,  BAIRD, Andrew ,  KASSNER, Paul

IPC分类号： C12N5/10 ,  C12Q1/68 ,  A61K38/00

CPC分类号： C40B40/02 ,  A61K47/6901 ,  C07K2319/00 ,  C12N15/1037 ,  C12N2799/021 ,  C12Q1/68 ,  C12Q1/6811 ,  C12Q1/6897

摘要： A genetic package display system and methodology for probing protein-protein interactions that lead to cell transduction, selecting and/or identifying internalizing ligands, target cells and tissues which internalize known or putative ligands, and cell transduction facilitating peptides is provided. A ligand displaying genetic package that carries a selectable marker (e.g., reporter, selection, etc.) and presents a ligand on its surface is utilized to identify internalizing ligands, transduction facilitating peptides, and/or a variety of cells and tissue types for the ability to be successfully transduced by the ligand displaying genetic package.