公开(公告)号：EP1621638A4

公开(公告)日：2007-05-09

申请号：EP04731764

申请日：2004-05-07

申请人： TAKARA BIO INC

发明人： TOMONO JUN ,  UENO HARUMI ,  TAKEDA OSAMU ,  MUKAI HIROYUKI ,  SAGAWA HIROAKI ,  KATO IKUNOSHIN

IPC分类号： C12Q1/68

CPC分类号： C12Q1/6844 ,  C12Q2527/107

摘要： A method of analyzing, in chromosomal DNA having a retroviral vector incorporated therein, DNA region derived from chromosome which is adjacent to the vector, characterized in that a primer extension reaction and a nucleic acid amplification reaction are carried out in the presence of a compound capable of lowering the Tm value of double stranded nucleic acid. There are further provided a kit and buffer for use in this method.

公开(公告)号：EP1396538A4

公开(公告)日：2005-10-26

申请号：EP02736063

申请日：2002-06-12

申请人： TAKARA BIO INC

发明人： SAGAWA HIROAKI ,  UEMORI TAKASHI ,  MUKAI HIROYUKI ,  YAMAMOTO JUNKO ,  TOMONO JUN ,  KOBAYASHI EIJI ,  ENOKI TATSUJI ,  ASADA KIYOZO ,  KATO IKUNOSHIN

IPC分类号： C12Q1/68 ,  C12N15/09

CPC分类号： C12Q1/6848 ,  C12Q2521/327 ,  C12Q2525/121 ,  C12Q2527/137

摘要： A method of stabilizing a reaction reagent for highly sensitively and specifically amplifying a target nucleic acid in a sample with the use of a chimeric oligonucleotide primer and a method of storing the same over a long time; and a method of highly sensitively detecting a pathogenic microorganism and a virus.

摘要翻译： 稳定的反应试剂为高灵敏度和特异性地与使用的嵌合寡核苷酸引物和在长时间存储相同的方法的样品中扩增靶核酸的方法; 和高灵敏度检测病原微生物和病毒的方法。

公开(公告)号：EP1424396A4

公开(公告)日：2006-08-16

申请号：EP02767888

申请日：2002-09-05

申请人： TAKARA BIO INC

发明人： UENO HARUMI ,  TOMONO JUN ,  SAGAWA HIROAKI ,  SAKAI TAKESHI ,  KATO IKUNOSHIN

IPC分类号： C12N1/19 ,  C12N1/21 ,  C12N9/24 ,  C12N9/40 ,  C12N15/56 ,  C12P19/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/14

CPC分类号： C12N9/2465 ,  C12N9/2402 ,  C12P19/00 ,  C12P19/04 ,  C12P19/12 ,  C12P19/14 ,  G01N2333/924

摘要： A gene encoding a polypeptide having an activity of digesting sulfated fucogalactan which is useful in sugar chain engineering reagents, analyzing the structures of sulfated fucose-containing polysaccharides and preparing degraded products of the polysaccharides; a genetic engineering process for producing the polypeptide; and the polypeptide obtained by the process.

公开(公告)号：EP1319710A4

公开(公告)日：2005-02-09

申请号：EP01965691

申请日：2001-09-18

申请人： TAKARA BIO INC

发明人： SAGAWA HIROAKI ,  ENOKI TATSUJI ,  KOBAYASHI EIJI ,  TOMONO JUN ,  KATO IKUNOSHIN

IPC分类号： C12N15/09 ,  C12Q1/68

CPC分类号： C12Q1/6839 ,  C12Q2533/101 ,  C12Q2525/113

摘要： A method of forming a complex consisting of a double-stranded nucleic acid and oligonucleotide(s) characterized by comprising: the step of mixing a double-stranded nucleic acid with at least one oligonucleotide to give a reaction mixture, wherein the oligonucleotide is a chimeric oligonucleotide containing at least a member selected from among deoxyribonucleotides and nucleotide analogs and a ribonucleotide and having a sequence substantially complementary to the base sequence of one of the strands of the double-stranded nucleic acid as described above; and the step of incubating the reaction mixture to form a complex under such conditions that the above-described double-stranded nucleic acid is not denatured.

公开(公告)号：EP1748071A4

公开(公告)日：2009-01-07

申请号：EP05741599

申请日：2005-05-19

申请人： TAKARA BIO INC

发明人： SHODAI TOSHIHIRO ,  KOBORI HIROSHI ,  SAGARA TAKEHIRO ,  ENDO HIROSHI ,  TAKAKURA HIKARU ,  TOMONO JUN ,  SAGAWA HIROAKI ,  MUKAI HIROYUKI ,  KATO IKUNOSHIN

IPC分类号： C12N15/09 ,  C12N9/12 ,  C12N9/22 ,  C12N15/70 ,  C12P21/02

CPC分类号： C12N15/70 ,  C12N9/1276 ,  C12N9/22 ,  C12P21/02

摘要： A process for producing a target protein at low temperature, comprising inducing expression of not only a vector having introduced therein a gene coding for the target protein but also a vector having a chaperone gene introduced therein.

公开(公告)号：EP1672060A4

公开(公告)日：2006-12-27

申请号：EP04788321

申请日：2004-09-29

申请人： TAKARA BIO INC

发明人： TOMONO JUN ,  UENO HARUMI ,  SAGAWA HIROAKI ,  KATO IKUNOSHIN

IPC分类号： C12P19/34 ,  C12N9/22 ,  C12N15/11 ,  C12N15/55

CPC分类号： C12N15/111 ,  C12N9/22 ,  C12N2310/14 ,  C12N2330/30 ,  C12Y301/26003

摘要： A polypeptide having an RNase III activity with which the length of a dsRNA degradation product can be easily controlled depending on reaction conditions and, in preparing a dsRNA having a length allowing it to serve as an siRNA in RNA interference, a low-molecular weight product having little RNA interferring effect is scarcely formed; a method of degrading a dsRNA with the use of the above polypeptide; and a composition and a kit for the above method.

公开(公告)号：EP1582587A4

公开(公告)日：2006-10-25

申请号：EP03778794

申请日：2003-12-11

申请人： TAKARA BIO INC

发明人： TOMONO JUN ,  UENO HARUMI ,  KISHIMOTO MASAYUKI ,  SAGAWA HIROAKI ,  KATO IKUNOSHIN

IPC分类号： C12N15/31 ,  C07K14/245 ,  C12N15/63 ,  C12N15/67 ,  C12N15/70

CPC分类号： C12N15/67 ,  C12N15/63

摘要： A vector having a region encoding a cold shock protein gene mRNA-origin 5'-nontranslated region, characterized in that the 5'-nontranslated region has a mutation having been transferred therein so as to change the distance of the stem structure formed by the region.

公开(公告)号：EP1365025A4

公开(公告)日：2004-08-25

申请号：EP02700773

申请日：2002-02-26

申请人： TAKARA BIO INC

发明人： TOMONO JUN ,  SAGAWA HIROAKI ,  KATO IKUNOSHIN

IPC分类号： C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N9/24 ,  C12N9/42 ,  C12N15/10 ,  C12N15/56 ,  C12N5/10 ,  C12N9/38 ,  C12N9/40 ,  C12P19/14

CPC分类号： C12Y302/01081 ,  C12N9/2468

摘要： Production of efficiently producing agaro-oligosaccharide; a polypeptide having an agarase activity which is usable in, for example, efficiently extracting a substance such as a nucleic acid from an agarose gel; the amino acid sequence of the polypeptide; a gene encoding the polypeptide; a process for producing the polypeptide; a process for producing agaro-oligosaccharide; and a process for extracting a substance such as a nucleic acid from an agarose gel.

公开(公告)号：EP1312682A4

公开(公告)日：2005-03-09

申请号：EP01956988

申请日：2001-08-21

申请人： TAKARA BIO INC

发明人： SAGAWA HIROAKI ,  UEMORI TAKASHI ,  MUKAI HIROYUKI ,  YAMAMOTO JUNKO ,  TOMONO JUN ,  KOBAYASHI EIJI ,  ENOKI TATSUJI ,  ASADA KIYOZO ,  KATO IKUNOSHIN

IPC分类号： C12N15/09 ,  C12N15/11 ,  C12Q1/68

CPC分类号： C12Q1/6853

摘要： A method of highly sensitively and specifically amplifying a target nucleic acid in a sample by using a chimeric oligonucleotide primer having a ribonucleotide provided at the 3'-terminus or in the 3'-terminal side, an endoribonuclease and a DNA polymerase having a chain transfer activity, i.e., an isothermal and chimeric primer-initiated amplification of nucleic acids (ICAN) method; a method of detecting an amplified fragment obtained by using the above method; a process for producing a target nucleic acid by using the above amplification method; and chimeric oligonucleotide primers to be used in these methods.

公开(公告)号：EP1086952A4

公开(公告)日：2005-02-02

申请号：EP99923961

申请日：1999-06-08

申请人： TAKARA BIO INC

发明人： ENOKI TATSUJI ,  TOMONO JUN ,  KOYAMA NOBUTO ,  IKAI KATSUSHIGE ,  SAGAWA HIROAKI ,  KATO IKUNOSHIN

IPC分类号： A23L1/30 ,  A23L3/3535 ,  A23L3/3562 ,  A61K31/35 ,  A61K31/351 ,  A61K31/357 ,  C07D493/08 ,  A23L2/00 ,  A61K7/00 ,  A61K31/12 ,  A61K31/52 ,  C07C45/52 ,  C07C45/59 ,  C07C47/263 ,  C07C49/707 ,  C07D309/32 ,  C07D473/18 ,  C07D473/34

CPC分类号： A61K31/7004 ,  A23L3/3535 ,  A23L3/3562 ,  A23L33/10 ,  A23V2002/00 ,  A61K31/35 ,  A61K31/351 ,  A61K31/357 ,  A23V2250/60 ,  A23V2250/608 ,  A23V2250/5024 ,  A23V2250/5036

摘要： Therapeutic or preventive agents for diseases requiring apoptosis induction, cancerous diseases, diseases requiring the inhibition of active oxygen production, those requiring the inhibition of nitrogen monoxide production, those requiring the inhibition of prostaglandin synthesis, those requiring the inhibition of synovial cell proliferation, those requiring the induction of heat shock protein production or those requiring the inhibition of α-glycosidase, which contain as the active ingredient compounds selected from among compounds represented by general formula (I), (wherein X and Y are each H or CH2OH, provided that when X is CH2OH, Y is H, while when X is H, Y is CH2OH), those represented by general formula (II), (wherein R is a residue obtained by freeing a compound having an SH group from the SH group) and salts of both; and foods, drinks, cosmetics and so on, containing compounds selected from among compounds of general formula (I), those of general formula (II) and salts of both.

摘要翻译： 需要凋亡诱导的疾病的治疗或预防剂，癌性疾病，需要抑制活性氧产生的疾病，需要抑制一氧化氮产生的那些，需要抑制前列腺素合成的那些，需要抑制滑膜细胞增殖的那些 ，需要诱导热休克蛋白产生的那些或需要抑制α-糖苷酶的那些，其含有选自通式（I）表示的化合物中的活性成分的化合物（其中X和Y各自为H或CH 2 OH， 条件是当X是CH 2 OH时，Y是H，而当X是H时，Y是CH 2 OH），由通式（II）表示的那些，其中R是从SH基团中释放具有SH基团的化合物获得的残基 ）和两者的盐; 和食品，饮料，化妆品等，含有选自通式（I）的化合物，通式（II）的化合物和二者的盐的化合物。