公开(公告)号：EP2350043B1

公开(公告)日：2014-03-26

申请号：EP09819991.2

申请日：2009-10-09

申请人： TEKMIRA Pharmaceuticals Corporation ,  The University of British Columbia

发明人： HOPE, Michael, J. ,  SEMPLE, Sean, C. ,  CHEN, Jianxin ,  MADDEN, Thomas, D. ,  CULLIS, Pieter, R. ,  CIUFOLINI, Marco, A. ,  MUI, Barbara

IPC分类号： C07D345/00 ,  C07D517/00 ,  A61K9/127

CPC分类号： A61K47/22 ,  A61K9/1271 ,  A61K31/713 ,  A61K39/39 ,  A61K2039/55511 ,  C07D317/28 ,  C07D319/06

公开(公告)号：EP1173600A2

公开(公告)日：2002-01-23

申请号：EP00920309.2

申请日：2000-04-20

申请人： THE UNIVERSITY OF BRITISH COLUMBIA

发明人： CULLIS, Pieter, R. ,  CHEN, Tao ,  FENSKE, David, B. ,  PALMER, Lorne, R. ,  WONG, Kim

IPC分类号： C12N15/88 ,  A61K47/48 ,  A61K9/127 ,  A61K49/00

CPC分类号： A61K48/00 ,  A61K9/1272 ,  A61K47/543 ,  A61K47/60 ,  A61K47/6911

摘要： The present invention provides cationic-polymer-lipid conjugates (CPLs) such as distal cationic-poly(ethylene glycol)-lipid conjugates which can be incorporated into conventional and stealth liposomes or other lipid-based formulation for enhancing cellular uptake. The CPLs of the present invention comprise a lipid moiety; a hydrophilic polymer; and a polycationic moiety. Method of increasing intracellular delivery of nucleic acids are also provided.

公开(公告)号：EP2496700B1

公开(公告)日：2017-03-01

申请号：EP10851175.9

申请日：2010-11-04

申请人： THE UNIVERSITY OF BRITISH COLUMBIA

发明人： CULLIS, Pieter, R. ,  BELLIVEAU, Nathan, M. ,  HANSEN, Carl Lars Genghis ,  HUFT, Jens ,  TAYLOR, James ,  WILD, Andre ,  MALCOM, Stuart ,  HAFEZ, Ismail ,  LEUNG, Alex ,  WALKER, David

IPC分类号： C12N15/87 ,  A61K47/22 ,  A61K47/24 ,  A61K47/34 ,  C12N15/00 ,  C12N15/10 ,  A61K31/7088 ,  C07H21/00 ,  C12N15/113 ,  C12N15/88 ,  A61K9/14

CPC分类号： C12N15/113 ,  A61K9/1272 ,  A61K9/145 ,  A61K9/146 ,  A61K31/7088 ,  A61K47/22 ,  C12N15/111 ,  C12N15/88 ,  C12N2310/14 ,  C12N2320/32

摘要： Lipid particles containing a nucleic acid, devices and methods for making the lipid particles, and methods for using the lipid particles.

摘要翻译： 含有核酸的脂质颗粒，用于制备脂质颗粒的装置和方法，以及使用脂质颗粒的方法。

公开(公告)号：EP2971013A1

公开(公告)日：2016-01-20

申请号：EP14785963.1

申请日：2014-03-14

申请人： The University of British Columbia

发明人： RAMSAY, Euan ,  TAYLOR, R., James ,  WALSH, Colin ,  BELLIVEAU, Nathan, M. ,  CULLIS, Pieter, R.

IPC分类号： C12N15/88

摘要： Transfection reagent composition, lipid nanoparticles prepared from the transfection reagent composition, kits that include the transfection reagent composition, and methods for making and using lipid nanoparticles prepared from the transfection reagent composition. Lipids when dispersed in aqueous media readily form liposomes, such as unilamellar vesicles and multilamellar vesicles. Liposomes have been used successfully to encapsulate and deliver a wide range of chemicals including nucleic acids, proteins and, small molecule drugs, to cells.

摘要翻译： 转染试剂组合物，由转染试剂组合物制备的脂质纳米颗粒，包括转染试剂组合物的试剂盒，以及由转染试剂组合物制备和使用脂质纳米颗粒的方法。 分散在水介质中的脂质容易形成脂质体，如单层囊泡和多层囊泡。 脂质体已被成功地用于包封和递送广泛的化学物质，包括核酸，蛋白质和。 小分子药物，细胞。

公开(公告)号：EP2224912A1

公开(公告)日：2010-09-08

申请号：EP08866209.3

申请日：2008-12-31

申请人： TEKMIRA Pharmaceuticals Corporation ,  THE UNIVERSITY OF BRITISH COLUMBIA ,  Alnylam Pharmaceuticals

发明人： HOPE, Michael, J. ,  SEMPLE, Sean, C. ,  CHEN, Jianxin ,  MADDEN, Thomas, D. ,  MUI, Barbara ,  CULLIS, Pieter, R. ,  CIUFOLINI, Marco, A. ,  WONG, Kim, F. ,  MANOHARAN, Muthiah ,  RAJEEV, Kallanthottathil, G. ,  SRINIVASULU, Masunu

IPC分类号： A61K9/127 ,  A61K31/7088

CPC分类号： A61K31/7088 ,  A61K9/127 ,  A61K9/1275 ,  C07C215/10 ,  C07C217/08 ,  C07C217/28 ,  C07C219/08 ,  C07C229/12 ,  C07C271/12 ,  C07C323/25 ,  C07D317/28

摘要： The present invention provides compositions and methods for the delivery of therapeutic agents to cells. In particular, these include novel lipids and nucleic acid-lipid particles that provide efficient encapsulation of nucleic acids and efficient delivery of the encapsulated nucleic acid to cells in vivo. The compositions of the present invention are highly potent, thereby allowing effective knock-down of specific target protein at relatively low doses. In addition, the compositions and methods of the present invention are less toxic and provide a greater therapeutic index compared to compositions and methods previously known in the art.

公开(公告)号：EP2496700A1

公开(公告)日：2012-09-12

申请号：EP10851175.9

申请日：2010-11-04

申请人： THE UNIVERSITY OF BRITISH COLUMBIA

发明人： CULLIS, Pieter, R. ,  BELLIVEAU, Nathan, M. ,  HANSEN, Carl Lars Genghis ,  HUFT, Jens ,  TAYLOR, James ,  WILD, Andre ,  MALCOM, Stuart ,  HAFEZ, Ismail ,  LEUNG, Alex ,  WALKER, David

IPC分类号： C12N15/87 ,  A61K47/22 ,  A61K47/24 ,  A61K47/34 ,  C12N15/00 ,  C12N15/10 ,  A61K31/7088 ,  C07H21/00 ,  C12N15/113 ,  C12N15/88 ,  A61K9/14

CPC分类号： C12N15/113 ,  A61K9/1272 ,  A61K9/145 ,  A61K9/146 ,  A61K31/7088 ,  A61K47/22 ,  C12N15/111 ,  C12N15/88 ,  C12N2310/14 ,  C12N2320/32

摘要： Lipid particles containing a nucleic acid, devices and methods for making the lipid particles, and methods for using the lipid particles.

公开(公告)号：EP1328254B1

公开(公告)日：2007-02-21

申请号：EP01983342.5

申请日：2001-10-25

申请人： THE UNIVERSITY OF BRITISH COLUMBIA ,  INEX Pharmaceuticals Corp.

发明人： CULLIS, Pieter, R. ,  MACLACHLAN, Ian ,  FENSKE, David, B.

IPC分类号： A61K9/127

CPC分类号： A61K9/1272

摘要： The present invention provides lipid-based systemic delivery vehicles and method for selectively targeting an active agent to a specific tissue site. The methods include designing a lipid-based systemic delivery vehicle having a plurality of constitutent parts, and thereafter varying the amounts of each of the plurality of constituent parts to impart tissue selectivity. After tissue selectivity is imparted it is possible to selectively target an active agent to a specific tissue site.

公开(公告)号：EP0941065A1

公开(公告)日：1999-09-15

申请号：EP97911086.0

申请日：1997-10-30

申请人： THE UNIVERSITY OF BRITISH COLUMBIA

发明人： FENSKE, David, B. ,  CULLIS, Pieter, R. ,  WONG, Kim ,  MAURER, Norbert ,  LEENHOUTS, Johanna, Maria ,  MAURER, Elisabeth ,  BOMAN, Nancy

IPC分类号： A61K9 ,  A61K31

CPC分类号： A61K9/1278

摘要： Novel methods are provided for loading a weakly basic drug into liposomes utilizing an electroneutral transport system. In these methods, ionophores are utilized with liposomes having a metal ion gradient to facilitate the exchange of metal ions for protons. The transported metal ion will, in some embodiments, be complexed with a chelating agent which is present in the external media.

公开(公告)号：EP2350043A1

公开(公告)日：2011-08-03

申请号：EP09819991.2

申请日：2009-10-09

申请人： TEKMIRA Pharmaceuticals Corporation ,  The University of British Columbia

发明人： HOPE, Michael, J. ,  SEMPLE, Sean, C. ,  CHEN, Jianxin ,  MADDEN, Thomas, D. ,  CULLIS, Pieter, R. ,  CIUFOLINI, Marco, A. ,  MUI, Barbara

IPC分类号： C07D345/00 ,  C07D517/00 ,  A61K9/127

CPC分类号： A61K47/22 ,  A61K9/1271 ,  A61K31/713 ,  A61K39/39 ,  A61K2039/55511 ,  C07D317/28 ,  C07D319/06

摘要： The present invention provides superior compositions and methods for the delivery of therapeutic agents to cells. In particular, these include novel lipids and nucleic acid-lipid particles that provide efficient encapsulation of nucleic acids and efficient delivery of the encapsulated nucleic acid to cells in vivo. The compositions of the present invention are highly potent, thereby allowing effective knock-down of specific target proteins at relatively low doses. In addition, the compositions and methods of the present invention are less toxic and provide a greater therapeutic index compared to compositions and methods previously known in the art.

公开(公告)号：EP0941065B1

公开(公告)日：2002-06-26

申请号：EP97911086.3

申请日：1997-10-30

申请人： THE UNIVERSITY OF BRITISH COLUMBIA

发明人： FENSKE, David, B. ,  CULLIS, Pieter, R. ,  WONG, Kim ,  MAURER, Norbert ,  LEENHOUTS, Johanna, Maria ,  MAURER, Elisabeth ,  BOMAN, Nancy

IPC分类号： A61K9/127

CPC分类号： A61K9/1278

摘要： Novel methods are provided for loading a weakly basic drug into liposomes utilizing an electroneutral transport system. In these methods, ionophores are utilized with liposomes having a metal ion gradient to facilitate the exchange of metal ions for protons. The transported metal ion will, in some embodiments, be complexed with a chelating agent which is present in the external media.