利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

7 条记录 (耗时 0.016 秒)

    FLUORESCENT AMP-KINASE BIOSENSORS
    1.
    发明公开
    FLUORESCENT AMP-KINASE BIOSENSORS 审中-公开
    荧光AMP-激酶生物传感器

    公开(公告)号：EP3158081A1

    公开(公告)日：2017-04-26

    申请号：EP15729200.4

    申请日：2015-06-18

    申请人： Université Grenoble Alpes ,  European Molecular Biology Laboratory

    发明人： BERGER, Imre ,  PELOSSE, Martin ,  SCHLATTNER, Uwe

    IPC分类号： C12Q1/48 ,  G01N33/542

    摘要： The present invention deals with heterotrimeric AMP-activated protein kinase (AMPK) comprising a fluorophore pair wherein the conformational change can be measured by FRET. It represents an advanced tool to screen and identify AMPK interactors in vitro and in cells in vivo. Such invention can also be considered as a reporter of the cellular energy status as it allows the spatiotemporal monitoring, in situ, of fluctuations in the ratio of AMP and ADP versus ATP.

    摘要翻译： 本发明涉及包含荧光团对的异源三聚AMP-活化蛋白激酶（AMPK），其中构象变化可以通过FRET测量。 它代表了一种高级工具，用于在体外和体内细胞中筛选和鉴定AMPK相互作用物。 这种发明也可以被认为是细胞能量状态的记者，因为它允许现场时空监测AMP和ADP与ATP之比的波动。

    TOOLS FOR MULTIPROTEIN COMPLEX EXPRESSION IN PICHIA PASTORIS
    3.
    发明公开
    TOOLS FOR MULTIPROTEIN COMPLEX EXPRESSION IN PICHIA PASTORIS 审中-公开
    PIC IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS IS

    公开(公告)号：EP3128006A1

    公开(公告)日：2017-02-08

    申请号：EP15179986.3

    申请日：2015-08-06

    申请人： Consejo Superior de Investigaciones Cientificas (CSIC) ,  EMBL (European Molecular Biology Laboratory)

    发明人： VEGA FERNÁNDEZ, María Cristina ,  FERNÁNDEZ PÉREZ, Francisco José ,  BERGER, Imre

    IPC分类号： C12N15/00

    CPC分类号： C12N15/815

    摘要： The invention provides an isolated multiple integration element (MIE) comprising, in the specified order: a) a homing endonuclease site (HE); b) a promoter selected from the group consisting of AOX1, FLD1, FMD1, GAP and TEF1; c) a BstBI restriction site; d) a sequence encoding a secretion signal; e) a Ncol restriction site; f) a selection marker gene; g) a Pmel restriction site; h) a DNA sequence encoding a detection and/or purification polypeptide; i) a terminator selected from the group consisting of AOX1, FLD1, FMD1, GAP and TEF1; and j) a BstXI restriction site, wherein the HE and BstXI sites are selected such that HE and BstXI result in compatible cohesive ends when cut by the homing endonuclease and the BstXI restriction enzyme, respectively, and the ligation product of HE and BstXI cohesive ends can neither be cleaved by the homing endonuclease nor the BstXI restriction enzyme.

    摘要翻译： 本发明提供一种分离的多重整合元件（MIE），其以指定的顺序包括：a）归巢内切核酸酶位点（HE）; b）选自AOX1，FLD1，FMD1，GAP和TEF1的启动子; c）BstBI限制性位点; d）编码分泌信号的序列; e）Nco限制性位点; f）选择标记基因; g）Pmel限制性位点; h）编码检测和/或纯化多肽的DNA序列; i）选自AOX1，FLD1，FMD1，GAP和TEF1的终止子; 和j）BstXI限制性位点，其中选择HE和BstXI位点，使得HE和BstXI分别在归巢内切核酸酶和BstXI限制酶切割时分别产生相容的粘性末端，并且HE和BstXI粘性末端的连接产物 不能被归巢内切核酸酶和BstXI限制酶切割。