-
公开(公告)号：EP3289066A1
公开(公告)日：2018-03-07
申请号：EP16725161.0
申请日：2016-05-27
CPC分类号： C12M23/16 , C12M3/04 , C12M3/065 , C12M21/08 , C12M23/06 , C12M29/04 , C12M29/12 , C12M35/08
摘要： Cell culture apparatus for emulating gastrointestinal tract conditions and comprising at least two adjacent, microfluidic, cell cultivation channels separated by a permeable or semipermeable membrane, a first channel carrying gastrointestinal tract epithelial cells or tissues and a second channel carrying luminal and preferably mucosal microbiota, and wherein said second channel comprises one or more dwell chambers capable of providing a location for unattached luminal flora to reside away from any direct flow in said second channel, permits modelling of multiple sections of the gastrointestinal tract and control of retention times.
-
公开(公告)号：EP2831221A1
公开(公告)日：2015-02-04
申请号：EP13713844.2
申请日：2013-03-27
申请人： The Arizona Board Of Regents On Behalf Of The University of Arizona , Université de Luxembourg , Centre de Recherche Public - Gabriel Lippmann
CPC分类号： C12M25/02 , B32B37/1292 , B32B38/1841 , B32B2307/726 , B32B2369/00 , C12M23/16 , C12M29/04 , C12M35/08 , C12N5/0693 , C12N2502/70
摘要： Cell culture apparatus comprising at least two adjacent cell cultivation channels separated by a permeable or semipermeable membrane, wherein at least one channel, for the majority of its length, has a cross sectional area of no more than 1 mm
2 , said channel being provided with entrance and exit means to permit the passage of media therethrough, allows co-culture of separate cell types,
e.g. human and microbial cells, without mingling, allowing monitoring of cell cultures and chemical exchanges between the respective cell cultures.-
公开(公告)号：EP2859119A2
公开(公告)日：2015-04-15
申请号：EP13731228.6
申请日：2013-06-12
发明人： GALAS, David , WANG, Kai , WILMES, Paul
CPC分类号： C12Q1/6895 , C12Q1/6888 , C12Q1/689 , C12Q2600/178 , G06F19/22 , G06F19/28
摘要： Determination of the level and identity of various RNA molecules in circulation or tissues of subjects permits determination of microbiome composition and function of the subject as well as providing an index to health of various organs. Because rapid parallel sequencing techniques are available, such determinations can be conducted on a practical level.
-
公开(公告)号：EP4058008A1
公开(公告)日：2022-09-21
申请号：EP20820786.0
申请日：2020-11-12
发明人： TREZZI, Jean-Pierre , WILMES, Paul
IPC分类号： A61K31/22 , A61K31/366 , A61K31/40 , A61K31/4045 , A61K31/47 , A61K31/505 , A61P25/16 , A61P43/00
-
公开(公告)号：EP3880795A1
公开(公告)日：2021-09-22
申请号：EP19802182.6
申请日：2019-11-15
发明人： WILMES, Paul , GREENHALGH, Kacy
-
6.发明授权METHOD FOR THE ISOLATION OF GENOMIC DNA, RNA, PROTEINS AND METABOLITES FROM A SINGLE BIOLOGICAL SAMPLE 有权式分离方法基因组DNA，RNA，蛋白质和代谢物从生物样品中
公开(公告)号：EP2751268B1
公开(公告)日：2016-04-06
申请号：EP12745471.8
申请日：2012-08-02
发明人： WILMES, Paul , ROUME, Hugo , HILLER, Karsten , CORDES, Thekla
CPC分类号： C12Q1/6806 , C12N1/066 , C12N15/1003 , C12N15/101 , G01N33/6803
-
公开(公告)号：EP4058808A1
公开(公告)日：2022-09-21
申请号：EP20820785.2
申请日：2020-11-12
发明人： TREZZI, Jean-Pierre , WILMES, Paul
IPC分类号： G01N33/68
-
8.发明公开METHOD AND KIT FOR THE ISOLATION OF GENOMIC DNA, RNA, PROTEINS AND METABOLITES FROM A SINGLE BIOLOGICAL SAMPLE 有权式分离方法基因组DNA，RNA，蛋白质和代谢物从生物样品中
公开(公告)号：EP2751268A1
公开(公告)日：2014-07-09
申请号：EP12745471.8
申请日：2012-08-02
发明人： WILMES, Paul , ROUME, Hugo , HILLER, Karsten , CORDES, Thekla
IPC分类号： C12N15/10
CPC分类号： C12Q1/6806 , C12N1/066 , C12N15/1003 , C12N15/101 , G01N33/6803
摘要： The invention provides method and kit for the separation and purification of cellular components including polar and non-polar metabolites, genomic DNA, RNA and proteins from a single biological sample where two steps of lysis of the cells are performed sequentially, before and after a metabolite isolation step. The first lysis step is mechanical and performed in order to be incomplete, whereas the second is chemical or both mechanical and chemical. A sequential isolation of genomic DNA, RNA and proteins is carried out after the second lysis step.
-
-
-
-
-
-
-
搜索结果
8 条记录 (耗时 0.022 秒)
