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公开(公告)号：EP1576151A2
公开(公告)日：2005-09-21
申请号：EP03817367.0
申请日：2003-08-06
IPC分类号： C12N9/00 , C12N1/20 , C12N15/00 , C12Q1/00 , C12Q1/34 , C12Q1/68 , C12P21/06 , C12P21/04 , A61K38/44 , C07H21/04 , C07K1/00
CPC分类号： C12N9/0022
摘要： New fumonisin detoxifying or fumonisin-derivative detoxifying homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, antibodies to the new homologues, and methods of using the homologues are also provided.
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公开(公告)号：EP1095149A2
公开(公告)日：2001-05-02
申请号：EP99933975.7
申请日：1999-07-14
CPC分类号： C12N15/8282 , A23K10/14 , A23K10/16 , A23K10/18 , A23K20/189 , A23K30/00 , A23K30/18 , C12N15/52 , C12N15/8242
摘要： Compositions and methods for the complete detoxification of fumonisin and fumonisin degradation products are provided. Particularly, nucleotide sequences corresponding to the detoxification enzymes are provided. The sequences find use in preparing expression cassettes for the transformation of a broad variety of host cells and organisms.
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3.发明公开MAIZE BASAL LAYER ANTIMICROBIAL PROTEIN POLYNUCLEOTIDES AND METHODS OF USE 审中-公开用于抗微生物基底层蛋白多核苷酸由玉米和使用方法
公开(公告)号：EP1417311A2
公开(公告)日：2004-05-12
申请号：EP02763374.2
申请日：2002-07-25
CPC分类号： C12N15/8279 , C07K14/415
摘要： Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding maize BTL proteins are provided. The sequence can be used in expression cassettes for antimicrobial resistance, modulating development, developmental pathways, and defense response. Transformed plants, plant cells, tissues, and seed are also provided.
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公开(公告)号：EP1185679A2
公开(公告)日：2002-03-13
申请号：EP00944778.0
申请日：2000-06-21
CPC分类号： C12N9/0032 , C07K14/415 , C12N15/8282
摘要： Compositions and methods to aid in protecting plants from invading pathogenic organisms are provided. The compositions of the invention comprise anti-pathogenic genes, including 5' regulatory sequences in the promoter, and proteins encoded by the anti-pathogenic genes. The compositions find use in methods for reducing or eliminating damage to plants caused by plant pathogens. Transformed plants, plant cells, tissues, and seed having enhanced disease resistance are also provided.
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公开(公告)号：EP1745148A1
公开(公告)日：2007-01-24
申请号：EP05735444.1
申请日：2005-04-11
CPC分类号： C12Q1/6897 , C12N15/1086
摘要： Methods and compositions for identifying a nucleotide sequence that encodes a polypeptide that directly or indirectly modulates the activity of a transcriptional regulatory region are provided. The methods permit the efficient screening of a large number of candidate nucleotide sequences for potential modulators of transcription. In particular, a population of polynucleotide constructs comprising a common transcriptional regulatory region operably linked to a candidate sequence is introduced into a population of host cells, and each candidate sequence is screened for modulation in its expression level, for example, using methods that employ hybridization to a complementary DNA microarray. The expression level of the candidate sequence correlates to the activity of the transcriptional regulatory region. Thus, alterations in the expression level of a candidate sequence indicate that the sequence encodes a polypeptide that modulates the activity of the transcriptional regulatory region. Compositions for practicing the invention are further provided.
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公开(公告)号：EP1196601A1
公开(公告)日：2002-04-17
申请号：EP99935536.5
申请日：1999-07-14
IPC分类号： C12N15/52 , C12N15/53 , C12N15/31 , C12N15/82 , A01H5/00 , A01N63/02 , A23K1/165 , A23L1/015
CPC分类号： C12N9/0071 , A23K20/189 , A23K30/18 , A23L5/25 , A23L5/28 , C07K14/37 , C07K14/39 , C12N9/00 , C12N9/0008 , C12N15/52 , C12N15/8242 , C12N15/8282
摘要： Compositions and methods for the complete detoxification of fumonisin and fumonisin degradation products are provided. Particularly, nucleotide sequences corresponding to the detoxification enzymes are provided. The sequences find use in preparing expression cassettes for the transformation of a broad variety of host cells and organisms.
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7.发明公开AMINO POLYOL AMINE OXIDASE POLYNUCLEOTIDES AND RELATED POLYPEPTIDES AND METHODS OF USE 审中-公开氨基多元醇胺氧化多核苷酸和相关的多肽和使用DeRene
公开(公告)号：EP1095151A1
公开(公告)日：2001-05-02
申请号：EP99933787.6
申请日：1999-07-08
CPC分类号： C12N9/0022 , A23K10/14 , A23K10/18 , A23K30/18 , C07K2319/00 , C12N9/16 , C12N15/8209 , C12N15/8242 , C12N15/8282 , G01N2333/906
摘要： The present invention provides polynucleotides and related polypeptides of the enzyme APAO isolated from Exophiala spinifera and Rhinocladiella atrovirens. Additionally, the polynucleotides encoding for the APAO enzyme can be used to transform plant cells normally susceptible to Fusarium or other toxin-producing fungus infection. Plants can be regenerated from the transformed plant cells. Additionally, the present invention provides for expressing both APAO and a fumonisin esterase in a transgenic plant. In this way, a transgenic plant can be produced with the capability of degrading fumonisin, as well as with the capability of producing the degrading enzymes. In addition, the present invention provides methods for producing the APAO enzyme in both prokaryotic and non-plant eukaryotic systems. Methods for detoxification in grain, grain processing, silage, food crops and in animal feed and rumen microbes are also disclosed.
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公开(公告)号：EP1745148B1
公开(公告)日：2010-06-09
申请号：EP05735444.1
申请日：2005-04-11
CPC分类号： C12Q1/6897 , C12N15/1086
摘要： Methods and compositions for identifying a nucleotide sequence that encodes a polypeptide that directly or indirectly modulates the activity of a transcriptional regulatory region are provided. The methods permit the efficient screening of a large number of candidate nucleotide sequences for potential modulators of transcription. In particular, a population of polynucleotide constructs comprising a common transcriptional regulatory region operably linked to a candidate sequence is introduced into a population of host cells, and each candidate sequence is screened for modulation in its expression level, for example, using methods that employ hybridization to a complementary DNA microarray. The expression level of the candidate sequence correlates to the activity of the transcriptional regulatory region. Thus, alterations in the expression level of a candidate sequence indicate that the sequence encodes a polypeptide that modulates the activity of the transcriptional regulatory region. Compositions for practicing the invention are further provided.
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9.发明公开SUNFLOWER RhoGAP, LOX, ADH, AND SCIP-1 POLYNUCLEOTIDES AND METHODS OF USE 审中-公开SUNFLOWER RhoGAP，LOX，ADH，与SCIP-1多核苷酸及其用途
公开(公告)号：EP1230372A2
公开(公告)日：2002-08-14
申请号：EP00977200.5
申请日：2000-11-13
发明人： BIDNEY, Dennis, L. , DUVICK, Jonathan, P. , HENDRICK, Carol , HU, Xu , LU, Guihua , CRASTA, Oswald, R.
CPC分类号： C12N9/0073 , C07K14/415 , C12N9/0006 , C12N15/8239 , C12N15/8261 , C12N15/8282 , Y02A40/146
摘要： Methods and compositions for modulating development and defense response are provided. Nucleotide sequences encoding a sunflower rhoGAP, LOX, ADH, and SCIP-1 protein are provided. Nucleotide sequences comprising the LOX and SCIP-1 promoters are also provided. The sequences can be used in expression cassettes for modulating development, developmental pathways, and the plant defense response. Transformed plants, plant cells, tissues, and seed are also provided.
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公开(公告)号：EP0832294A1
公开(公告)日：1998-04-01
申请号：EP96921259.0
申请日：1996-06-05
CPC分类号： C12Q1/6809 , C07K14/415 , C12N15/1096 , C12Q2539/107 , C12Q2525/186 , C12Q2563/131
摘要： A process is described in which PCR-based cDNA libraries with anchored ends are made, a PCR--based cDNA subtracted (PCSUB) library is produced therefrom, and the ends of a cDNA clone are isolated, via PCR, from an anchored-ends library.
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