公开(公告)号：EP2029739A2

公开(公告)日：2009-03-04

申请号：EP07873501.6

申请日：2007-05-29

申请人： Verenium Corporation

发明人： WEINER, David ,  VARVAK, Alexander ,  RICHARDSON, Toby ,  PODAR, Mircea ,  BURKE, Ellen ,  HEALEY, Shaun

IPC分类号： C12N9/88

CPC分类号： C12N9/88

摘要： This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts.

公开(公告)号：EP1989302A2

公开(公告)日：2008-11-12

申请号：EP07751203.6

申请日：2007-02-14

申请人： Verenium Corporation

发明人： WEINER, David ,  BLUM, David ,  HEALEY, Shaun ,  VARVAK, Alexander ,  TODARO, Thomas ,  HAZLEWOOD, Geoff ,  CHANG, Kristine ,  CHANG, Hwai ,  DESANTIS, Grace ,  HANSEN, Connie Jo ,  HANCOCK, Charles ,  WOODWARD, Thomas ,  BEAVER, Scott W.

IPC分类号： C12N9/42

CPC分类号： C12N9/248 ,  A23K10/12 ,  A23K20/163 ,  A23L5/00 ,  A23L7/00 ,  A23L29/30 ,  A23L31/00 ,  A23L31/10 ,  A23L33/125 ,  A23V2002/00 ,  C12N9/2405 ,  C12N9/2437 ,  C12N9/2482 ,  C12N9/2488 ,  C12N9/2494 ,  C12P19/02 ,  C12P19/14 ,  C12Y302/01008 ,  D06L4/40 ,  D06L4/70 ,  D21C3/003 ,  D21C9/10 ,  D21H17/005 ,  Y02E50/16

摘要： The invention relates to enzymes having xylanase, mannanase and/or glucanase activity, e.g., catalyzing hydrolysis of internal ²-1,4-xylosidic linkages or endo- ²-1,4-glucanase linkages; and/or degrading a linear polysaccharide beta-1,4-xylan into xylose. Thus, the invention provides methods and processes for breaking down hemicellulose, which is a major component of the cell wall of plants, including methods and processes for hydrolyzing hemicelluloses in any plant or wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. In addition, methods of designing new xylanases, mannanases and/or glucanases and methods of use thereof are also provided. The xylanases, mannanases and/or glucanases have increased activity and stability at increased pH and temperature.

公开(公告)号：EP1869174A2

公开(公告)日：2007-12-26

申请号：EP06748348.7

申请日：2006-03-10

申请人： Verenium Corporation

发明人： WEINER, David ,  VARVAK, Alexander ,  RICHARDSON, Toby ,  PODAR, Mircea ,  BURKE, Ellen ,  HEALEY, Shaun

IPC分类号： C12N9/88

CPC分类号： C12N9/88 ,  C12P7/42 ,  C12P13/222 ,  C12P13/225 ,  C12P13/24 ,  C12Y403/01005 ,  C12Y403/01023 ,  D06M16/003

摘要： This invention provides polypeptides having lyase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In one aspect, the invention is directed to polypeptides having ammonia lyase activity, e.g., phenylalanine ammonia lyase, tyrosine ammonia lyase and/or histidine ammonia lyase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides of the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. X-NO2, Cl, Br, F, NH2, OH, H, alkyl at one or several o, m, and p positions R-H or alkyl