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公开(公告)号：EP3323880A1
公开(公告)日：2018-05-23
申请号：EP16836577.3
申请日：2016-08-09
发明人： YE, Lei
IPC分类号： C12M3/02
CPC分类号： C12M3/02 , C12M3/04 , C12M23/08 , C12M23/16 , C12M25/14 , C12M25/18 , C12N5/0062 , C12N5/0068
摘要： The present invention relates to a culture device for tissue cell suspension. The culture device includes a tissue cell culture body. The tissue cell culture body is a porous material. The porous material is formed by cavities classified into different levels according to the pore size of material and cavity walls surrounding to form the cavities. A lower level of small cavities is provided to surround the cavity wall which forms the upper level of large cavity. Cavities of each level are in communication with each other, and cavities between respective levels are also in communication with each other. The culture device further includes a swirler device. Such culture device can particularly facilitate normal and unrestricted growth of the suspension of cells in three-dimensional space, obstructing the formation of over-dense cell region or nutrient-rich region during the cell culturing, enabling a uniform cell distribution, and facilitating normal growth of cells.
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2.发明公开LIQUID DELIVERY METHOD FOR CELL CULTURE SYSTEM, AND CELL CULTURE SYSTEM 审中-公开用于细胞培养系统的液体传递方法和细胞培养系统
公开(公告)号：EP3196289A1
公开(公告)日：2017-07-26
申请号：EP15842148.7
申请日：2015-09-09
发明人： KOSEKI, Osamu , SUENAGA, Ryo , KASHIWABARA, Ken
摘要： A cell culture system disclosed herein comprises a culture medium container (10) to supply a culture medium to a culture container, two culture containers (20, 30) for use in cell culture, and a transfer tube (40) to connect these containers, whereby liquid delivery can be performed by use of only one pump (41). The containers in the cell culture system are connected to the transfer tube (40) in the order of the culture medium container (10), the second culture container (30) and the first culture container (20); the only one pump (41) is attached to the transfer tube; the second culture container is connected to the transfer tube on both sides of the pump by culture container connecting tubes (42) to connect the second culture container and the transfer tube, to inject cells into the first culture container; the culture medium is transferred from the culture medium container to the first culture container by the pump; after the cell culture in the first culture container, a cell suspension is transferred from the first culture container to the second culture container; and the culture medium is transferred from the culture medium container to the second culture container.
摘要翻译： 本文公开的细胞培养系统包括向培养容器供应培养基的培养基容器（10），用于细胞培养的两个培养容器（20,30）和连接这些容器的输送管（40） 由此可以仅通过使用一个泵（41）来执行液体输送。 细胞培养系统中的容器按照培养基容器（10），第二培养容器（30）和第一培养容器（20）的顺序与输送管（40）连接; 唯一一个泵（41）连接到输送管上; 第二培养容器通过培养容器连接管（42）与泵两侧的传送管连接，连接第二培养容器和传送管，将细胞注入第一培养容器; 通过泵将培养基从培养基容器转移至第一培养容器; 在第一培养容器中进行细胞培养之后，将细胞悬浮液从第一培养容器转移到第二培养容器中; 并且培养基从培养基容器转移到第二培养容器。
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公开(公告)号：EP2710135A2
公开(公告)日：2014-03-26
申请号：EP12730011.9
申请日：2012-05-18
CPC分类号： C12P7/6409 , A01C3/023 , A01H4/001 , C12M1/002 , C12M3/02 , C12M21/02 , C12M23/58 , C12N1/12 , C12N5/04 , C12P7/06 , C12P7/64 , C12P19/02 , C12P19/12 , C12R1/89 , C12R1/91 , Y02E50/17
摘要： The present invention provides a method of producing a photosynthetic product, the method comprising maintaining a photosynthetic plant or algal cell suspension culture, in the presence of water, light and a carbonic acid-enriched growth medium. The carbonic acid may, for example be provided by feeding the photosynthetic plant cell suspension culture with a carbonic acid solution, a solid or liquid precursor thereof, or a gaseous mixture of carbon dioxide and one or more other gases. The invention also provides a method for producing a photosynthetic product, the method comprising maintaining a photosynthetic plant or algal cell suspension culture, in the presence of water, light and a carbon source selected from carbon dioxide and carbonic acid, wherein the culture is maintained at a pH of less than 7.0, preferably 4.5 to 5.5.
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公开(公告)号：EP3287514A1
公开(公告)日：2018-02-28
申请号：EP16764728.8
申请日：2016-03-03
发明人： KOIKE, Tetsuo , TAKIMOTO, Masahiro , YAGI, Yoshiki
IPC分类号： C12M1/00
CPC分类号： C12M41/36 , C12M1/00 , C12M1/34 , C12M1/36 , C12M3/02 , C12M41/46 , C12M41/48 , C12N5/06 , C12R1/91 , G06T1/0014
摘要： Provided is an apparatus to produce cultured cell products including: an isolator 3 configured to maintain its inside in aseptic conditions and process cell culture vessels 1 therein; and at least one robot arm 6, 7, and 10 to 12 located within the isolator 3, wherein a taking-out step of taking out cells cultured in the cell culture vessels 1, a cell density-adjusting step of adjusting density of the cells in a cell-containing liquid containing the taken-out cells, and a subdividing step of subdividing and placing the cell-containing liquid with its density adjusted into a plurality of product containers 9 are performed within the isolator 3 by the at least one robot arm 6, 7, and 10 to 12.
摘要翻译： 本发明提供一种生产培养细胞产品的设备，包括：隔离器3，其被配置为将其内部保持在无菌条件下并且在其中处理细胞培养容器1; 以及位于隔离器3内的至少一个机器人手臂6,7和10至12，其中取出步骤取出在细胞培养容器1中培养的细胞;细胞密度调整步骤，调整细胞的密度 在包含被取出的细胞的含细胞液体中，并且通过至少一个机器人臂在隔离器3内执行将细胞含有液体的密度调整并放置到多个产品容器9中的细分步骤（细分步骤） 6日，7日和10日至12日。
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5.发明公开METHOD OF LOADING AND DISTRIBUTING CELLS IN A BIOREACTOR OF A CELL EXPANSION SYSTEM 审中-公开方法用于加载和细胞分布的生物反应器细胞扩增系统
公开(公告)号：EP2885393A1
公开(公告)日：2015-06-24
申请号：EP13756956.2
申请日：2013-08-20
申请人： Terumo BCT, Inc.
发明人： NANKERVIS, Brian, J.
CPC分类号： C12M41/44 , C12M3/00 , C12M3/02 , C12M21/08 , C12M23/08 , C12M25/12 , C12M27/10 , C12M29/04 , C12M29/18 , C12M41/00 , C12M47/04 , C12N5/00 , C12N5/0081
摘要： One or more embodiments are described directed to a method and system for loading and distributing cells in a bioreactor of a cell expansion system. Accordingly, embodiments include methods and systems that may provide for adding a plurality of cells to a fluid within a bioreactor of the cell expansion system. A first percentage of the plurality of cells is allowed to settle in the bioreactor and a second percentage of the plurality of cells is allowed to settle outside of the bioreactor. The first percentage of cells is then expanded in the bioreactor. The second percentage of cells is wasted.
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公开(公告)号：EP4424816A1
公开(公告)日：2024-09-04
申请号：EP22887175.2
申请日：2022-10-28
发明人： MOCHIZUKI Shinsuke , KATO Mai , TSUBAKI Keiichiro , TOMATSU Fumiko , YAMAUCHI Fumio , KANAZAKI Kengo
CPC分类号： C12N5/00 , C12Q1/24 , C12N1/04 , C12N5/06 , C12M1/26 , C12N1/02 , C12M3/02 , C12M1/00 , C12N1/00
摘要： A cell dissociation solution is used in dissociation of, from a substrate, a cell disposed on the substrate, by application of ultrasonic vibration to the cell and the cell dissociation solution contains a polymer including a polyalkylene glycol structure.
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公开(公告)号：EP3305885A1
公开(公告)日：2018-04-11
申请号：EP16800017.2
申请日：2016-05-24
申请人： Nipro Corporation
CPC分类号： C12M3/043 , B01L3/50 , B01L3/563 , B01L2200/025 , B01L2200/026 , B01L2300/046 , C12M1/007 , C12M1/065 , C12M1/10 , C12M1/38 , C12M3/02 , C12M23/14 , C12M23/34 , C12M23/42 , C12M23/44 , C12M23/50 , C12M29/00 , C12M41/48
摘要： [Object] To provide a compact culture device 10 capable of performing large-scale culture in an aseptic state.
[Solution] The culture device 10 has a culture bag 70 which has ports 73 and 74 through which a liquid flows and enables cell culture, a bag holding portion 31 having holding plates 42 and 43 holding the culture bag 70 and rotating shafts 48 and 49, a rotation mechanism 34 supporting and rotating the rotating shaft 48, a liquid supply/discharge mechanism 37 supplying and discharging a liquid to/from the culture bag 70, and a rotation control portion 20 controlling the rotation of the rotation mechanism 34. The rotation control portion 20 brings the bag holding portion 31 into a first position in parallel with the approximately horizontal direction according to a first information, brings the bag holding portion 31 into a second position when a culture medium in the culture bag 70 is caused to flow out of the port 74 according to a second information, and brings the bag holding portion 31 into a third position in parallel with the approximately vertical direction at least when a cell suspension in the culture bag 70 is caused to flow out of the port 74 according to a third information.摘要翻译： 本发明的目的是提供一种能够在无菌状态下进行大规模培养的小型培养装置10。 培养装置10具有培养袋70，该培养袋70具有供液体流动并能够进行细胞培养的端口73,74;袋保持部31，其具有保持培养袋70的保持板42,43;以及旋转轴48,49 ，支承旋转轴48并使其旋转的旋转机构34，向培养袋70供给液体并从培养袋70排出液体的液体供给排出机构37，以及控制旋转机构34的旋转的旋转控制部20。 控制部20根据第一信息使袋保持部31进入与大致水平方向平行的第一位置，当使培养袋70中的培养基流出时使袋保持部31进入第二位置 根据第二信息，将袋保持部31置于与大致垂直方向平行的第三位置，至少在单元su 根据第三信息使培养袋70中的张力从端口74流出。
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公开(公告)号：EP1620537A2
公开(公告)日：2006-02-01
申请号：EP04720783.2
申请日：2004-03-15
申请人： Walters, Richard E. , King, Alan D.
发明人： Walters, Richard E. , King, Alan D.
IPC分类号： C12N1/00
CPC分类号： C12N13/00 , A61K9/0009 , C12M1/42 , C12M3/00 , C12M3/006 , C12M3/02 , C12M35/02 , C12M45/07 , C12N15/87
摘要： An object of the invention is to provide an electroporation method for treating vesicles with exogenous material for insertion of the exogenous material into the vesicles which includes the steps of: a. retaining a suspension of the vesicles and the exogenous material in a treatment volume in a chamber which includes electrodes, wherein the chamber has a geometric factor (cm -1 ) defined by the quotient of the electrode gap squared (cm 2 ) divided by the chamber volume (cm 3 ), wherein the geometric factor is less than or equal to 0.1 cm -1 , wherein the suspension of the vesicles and the exogenous material is in a medium which is adjusted such that the medium has conductivity in a range spanning 0.01 to 1.0 milliSiemens, wherein the suspension is enclosed in the chamber during treatment, and h. treating the suspension enclosed in the chamber with one or more pulsed electric fields. With the method, the treatment volume of the suspension is scalable, and the time of treatment of the vesicles in the chamber is substantially uniform.
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公开(公告)号：EP3252153A4
公开(公告)日：2018-07-18
申请号：EP16743340
申请日：2016-01-26
申请人： KANEKA CORP , UNIV TOKYO
发明人： HORIGUCHI IKKI , SAKAI YASUYUKI , IBUKI MASATO
IPC分类号： C12N5/10 , C12M3/02 , C12N5/0735
CPC分类号： C12M3/02 , C12N5/0018 , C12N5/10 , C12N2500/72 , C12N2525/00
摘要： The present invention addresses the problem of providing a method for producing cell aggregates using suspension culture, wherein it is easy to control the size of the cell aggregates so as to be appropriate for culturing, and the likelihood of damaging the cells is low. The present invention pertains to a method for producing cell aggregates, the method comprising the step of culturing cells while suspended in a liquid culture medium containing a lysophospholipid.
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公开(公告)号：EP3196289A4
公开(公告)日：2018-06-06
申请号：EP15842148
申请日：2015-09-09
发明人： KOSEKI OSAMU , SUENAGA RYO , KASHIWABARA KEN
摘要： A cell culture system disclosed herein comprises a culture medium container (10) to supply a culture medium to a culture container, two culture containers (20, 30) for use in cell culture, and a transfer tube (40) to connect these containers, whereby liquid delivery can be performed by use of only one pump (41). The containers in the cell culture system are connected to the transfer tube (40) in the order of the culture medium container (10), the second culture container (30) and the first culture container (20); the only one pump (41) is attached to the transfer tube; the second culture container is connected to the transfer tube on both sides of the pump by culture container connecting tubes (42) to connect the second culture container and the transfer tube, to inject cells into the first culture container; the culture medium is transferred from the culture medium container to the first culture container by the pump; after the cell culture in the first culture container, a cell suspension is transferred from the first culture container to the second culture container; and the culture medium is transferred from the culture medium container to the second culture container.
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