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    A SIMPLE TWO PLASMID MAMMALIAN EXPRESSION SYSTEM FOR PRODUCTION OF RECOMBINANT PROTEINS AND VIRUSES
    1.
    发明公开
    A SIMPLE TWO PLASMID MAMMALIAN EXPRESSION SYSTEM FOR PRODUCTION OF RECOMBINANT PROTEINS AND VIRUSES 审中-公开
    带有两个质粒SIMPLE哺乳动物表达系统生产重组蛋白和病毒

    公开(公告)号：EP2718445A2

    公开(公告)日：2014-04-16

    申请号：EP12835226.7

    申请日：2012-06-08

    申请人： Joshi, Vishwas

    发明人： Joshi, Vishwas

    IPC分类号： C12N15/86

    CPC分类号： C12N7/00 ,  A61K39/00 ,  C12N15/85 ,  C12N15/86 ,  C12N2760/00051 ,  C12N2760/18451 ,  C12N2800/40 ,  C12N2840/203

    摘要： Reverse engineering has offered new ways of studying the pathobiology of RNA viral infections, new more efficient means of synthesizing recombinant viruses and developing vaccines and also demonstrated the versatility and efficiency of RNA dependent RNA polymerase RDRP system as an expression system. However, the currently used methods require a repertoire of complex, difficult-to- use tools. Present invention describes, a simpler plasmid based mammalian expression system that uses the RDRP enzyme activity for expression of recombinant proteins or RNA from viral minigenomes and rescue of recombinant viruses from cDNAs encoding entire genome(s) of negative stranded RNA viruses. This system will be useful for expression of recombinant proteins, therapeutic RNA molecules including anti-sense and/or selective interefering RNA and Ribozymes. This system can also be used for gene therapy and producing recombinant viruses for production of new vaccines.

    摘要翻译： 逆向工程已提供的研究RNA病毒感染，合成重组病毒的新的更有效的器件的病理学和开发疫苗的新方法，因此实例阐述了通用性和RNA依赖性RNA聚合酶RDRP系统的效率，因为在表达系统。然而，目前使用的 方法需要的复杂，难于使用的工具剧目。 本发明描述了一个简单的基于质粒的哺乳动物表达系统使用没有为重组蛋白的表达或RNA的RDRP酶活性从病毒基因组mini和从编码负链RNA病毒完整基因组（多个）的cDNA的重组病毒拯救。 该系统将用于重组蛋白，治疗性RNA分子，包括反义和/或选择的干扰RNA和核酶的表达是有用的。 因此，该系统可被用于基因治疗和产生重组病毒生产的新疫苗。