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    Isolation of microbial oils
    8.
    发明专利
    Isolation of microbial oils 审中-公开
    分离微生物油

    公开(公告)号：JP2014138598A

    公开(公告)日：2014-07-31

    申请号：JP2014031850

    申请日：2014-02-21

    申请人： Dsm Ip Assets Bv ,  ディーエスエム アイピー アセッツ ビー.ブイ.

    发明人： BIJL HENDRIK LOUIS ,  SCHAAP ALBERT

    IPC分类号： C12M1/00 ,  C12P7/64 ,  A23D9/00 ,  B01D17/038 ,  C07G17/00 ,  C11B1/00 ,  C11B1/02 ,  C11B1/06

    CPC分类号： C12P7/6463 ,  A23D9/00 ,  C11B1/00 ,  C11B1/025 ,  C11B1/06 ,  C12P7/6472

    摘要： PROBLEM TO BE SOLVED: To provide a method for directly extracting microbial or unicellular oils containing one or more polyunsaturated fatty acids (PUFAs) and the like from microbial cells without the need of a solvent.SOLUTION: After fermentation, microbial cells are pasteurised and washed, and the cell walls are lysed or disrupted by a mechanical (e.g. homogenization), physical (boiling or drying), chemical (solvents) or enzymatic (cell wall degrading enzymes) technique. The oil (containing the PUFA) is then separated from the resulting cell wall debris. This is achieved by centrifugation, which results in an oily phase (top layer) that contains the oil. The oily phase is separated from an aqueous phase (containing the cell wall debris). The oil is then extracted and if necessary the PUFA is purified or isolated from the oil.

    摘要翻译： 要解决的问题：提供从微生物细胞中直接提取含有一种或多种多不饱和脂肪酸（PUFA）等的微生物或单细胞油的方法，而不需要溶剂。解决方案：发酵后，将微生物细胞进行巴氏消毒和洗涤 ，并且通过机械（例如均化），物理（沸腾或干燥），化学（溶剂）或酶（细胞壁降解酶）技术裂解或破坏细胞壁。 然后将油（含有PUFA）与所得的细胞壁碎片分离。 这通过离心实现，其导致含有油的油相（顶层）。 油相与水相分离（含有细胞壁碎片）。 然后萃取油，如果需要，将PUFA从油中纯化或分离。

    Extraction and winterization of lipid from oilseed and microbial source
    9.
    发明专利
    Extraction and winterization of lipid from oilseed and microbial source 审中-公开
    来自油和微生物来源的脂肪的提取和冷冻

    公开(公告)号：JP2013100530A

    公开(公告)日：2013-05-23

    申请号：JP2013007324

    申请日：2013-01-18

    申请人： Dsm Ip Assets Bv ,  ディーエスエム アイピー アセッツ ビー.ブイ.

    发明人： DUEPPEN DANIEL G ,  ZELLER SAMUEL G ,  DILTZ SANDRA I ,  DRIVER ROBERT H

    IPC分类号： C11B7/00 ,  C12N15/09 ,  A23D9/02 ,  B01D11/02 ,  C07C51/48 ,  C07C57/03 ,  C11B1/00 ,  C11B1/10 ,  C11B3/00 ,  C11B3/16 ,  C12P7/64 ,  C12R1/89

    CPC分类号： C11B1/00 ,  A23V2002/00 ,  C11B1/10 ,  C11B3/006 ,  C11B7/0025 ,  A23V2250/1942

    摘要： PROBLEM TO BE SOLVED: To provide a process for purifying a lipid composition having predominantly neutral lipid components having at least one long chain polyunsaturated fatty acid.SOLUTION: The process includes contacting the lipid composition with a polar solvent, such as acetone, wherein the solvent is selected such that contaminants are less soluble in the solvent than in the long chain polyunsaturated fatty acid. The contact process is typically conducted at cooler temperatures, including about 0°C. Upon precipitation of the contaminants from the lipid composition, a separation is conducted to remove the precipitated material from the lipid composition. The long chain polyunsaturated fatty acids can include ARA, DPA, EPA, and/or DHA. The process effectively winterizes lipid compositions, thereby reducing the tendency of such compositions to become hazy.

    摘要翻译： 要解决的问题：提供一种纯化具有主要具有至少一种长链多不饱和脂肪酸的中性脂质组分的脂质组合物的方法。 解决方案：该方法包括使脂质组合物与极性溶剂如丙酮接触，其中选择溶剂使得污染物在溶剂中的溶解度比长链多不饱和脂肪酸中的溶解度低。 接触过程通常在较冷的温度下进行，包括约0℃。 当从脂质组合物沉淀污染物时，进行分离以从脂质组合物中除去沉淀的物质。 长链多不饱和脂肪酸可以包括ARA，DPA，EPA和/或DHA。 该方法有效地使脂质组合物变凉，从而降低这些组合物变得模糊的倾向。 版权所有（C）2013，JPO＆INPIT