Cell culture
    71.
    发明专利
    Cell culture 有权
    细胞培养

    公开(公告)号:JP2011250791A

    公开(公告)日:2011-12-15

    申请号:JP2011155433

    申请日:2011-07-14

    发明人: CHOO YEN

    摘要: PROBLEM TO BE SOLVED: To provide improved technologies for regulating cell processes such as culturing, proliferating, differentiating, metabolic activity and expression of phenotype, etc., of the cells.SOLUTION: This method for obtaining differentiated cells from stem cells in vitro comprises (a) a step of proliferating the stem cells attached to micro-carriers or beads, or enclosed or captured in a medium penetrable barrier in a culturing medium, (b) a step of moving the micro-carriers of the cells in the step (a) from one culturing medium to another culturing medium, (c) a step of repeating the step (b) as necessary by an optional selection, and (d) a step of obtaining the differentiated cells.

    摘要翻译: 要解决的问题:提供用于调节细胞的细胞过程的改进技术,例如细胞的培养,增殖,分化,代谢活性和表型表达等。 解决方案:用于在体外从干细胞获得分化细胞的该方法包括(a)增殖附着于微载体或珠的干细胞或包封或捕获在培养基中的介质穿透性屏障中的步骤( b)将步骤(a)中的细胞的微载体从一种培养基移动到另一培养基的步骤,(c)根据需要通过任选的选择重复步骤(b)的步骤,和(d )获得分化细胞的步骤。 版权所有(C)2012,JPO&INPIT