公开(公告)号：US20140344999A1

公开(公告)日：2014-11-20

申请号：US14451659

申请日：2014-08-05

申请人： Nadine Carozzi ,  Michael G. Koziel ,  Nicholas Duck ,  Nalini M. Desai ,  Rong Guo ,  Daniel John Tomso ,  Rebekah Deter ,  Tracy Hargiss

发明人： Nadine Carozzi ,  Michael G. Koziel ,  Nicholas Duck ,  Nalini M. Desai ,  Rong Guo ,  Daniel John Tomso ,  Rebekah Deter ,  Tracy Hargiss

IPC分类号： C12N15/82 ,  C07K14/325 ,  C12P21/02 ,  A01N37/44

CPC分类号： C12N15/8286 ,  A01N37/44 ,  C07K14/325 ,  C12P21/02 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for pesticidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated pesticidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 2, 4, 6, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37 or 61, the nucleotide sequence set forth in SEQ ID NO: 1, 3, 5, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, 34, 36, or 60, or the nucleotide sequence deposited in a bacterial host as Accession No. B-30955, B-30956, B-30957, B-30958, B-30942, B-30939, B-30941, B-50047, B-30959, B-30960, B-30943, or B-50048, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含杀虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀虫核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO： 25，27，29，31，33，35，37或61所述的核苷酸序列，所述核苷酸序列如SEQ ID NO：1,3,5,8,10,12,14,16,18,20,22,24， 26,28,30,32,34,36或60个核苷酸序列，或作为保藏号B-30955，B-30956，B-30957，B-30958，B-30942，B- 30939，B-30941，B-50047，B-30959，B-30960，B-30943或B-50048，以及其变体和片段。

公开(公告)号：US08541366B2

公开(公告)日：2013-09-24

申请号：US12209354

申请日：2008-09-12

申请人： Nadine Carozzi ,  Nalini Desai ,  Daniel J. Tomso ,  Vadim Beilinson ,  Theodore Kahn

发明人： Nadine Carozzi ,  Nalini Desai ,  Daniel J. Tomso ,  Vadim Beilinson ,  Theodore Kahn

IPC分类号： A01N37/18 ,  C07K14/325

CPC分类号： C07K14/325 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided, particularly synthetically-derived coding sequences. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, or 18, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 4, 6, 7, 8, 10, 12, 14, 16, or 17, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供包含δ-内毒素多肽的编码序列的组合物，特别是合成来源的编码序列。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：9,11,13,15或18所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2，4，6，7，8，10，12，14，16或17，以及其变体和片段。

公开(公告)号：US20130133107A1

公开(公告)日：2013-05-23

申请号：US13747740

申请日：2013-01-23

申请人： Athenix Corporation

发明人： Cheryl L. Peters ,  Brian Vande Berg ,  Brian Carr ,  Julia T. Daum ,  Vadim Beilinson ,  Sandra Volrath ,  Candace Poutre ,  Kimberly Sampson

IPC分类号： C12N15/82

CPC分类号： C12N15/8241 ,  C12N9/0059 ,  C12N9/0071 ,  C12N15/8285 ,  C12Y110/03001 ,  C12Y114/18001 ,  Y02A40/164

摘要： Compositions and methods for conferring nematicidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions including a coding sequence for nematicidal polypeptides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated nematicidal nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules including nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 8, 9, 13, 14, 47, 48, or 49, the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 6, 7, 10, 11, 12, 15, 45, or 46, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀线虫活性的组合物和方法。 提供了包含杀线虫多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的杀线虫剂核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包括编码SEQ ID NO：4,5,8,9,13,14,77,48或49所示氨基酸序列的核苷酸序列，所列出的核苷酸序列 在SEQ ID NO：1,2,3,6,7,10,11，12，15，45或46中，以及其变体和片段。

公开(公告)号：US08426204B2

公开(公告)日：2013-04-23

申请号：US12555175

申请日：2009-09-08

申请人： Philip E. Hammer ,  Vadim Beilinson ,  Todd K. Hinson

发明人： Philip E. Hammer ,  Vadim Beilinson ,  Todd K. Hinson

IPC分类号： A01H5/00 ,  C12N5/04 ,  C12N15/82

CPC分类号： C12N15/8279 ,  C12N15/8214 ,  C12N15/8274

摘要： The present invention provides compositions and methods for targeting polypeptides to the chloroplasts of higher plants. Compositions include expression cassettes having a nucleotide sequence encoding a chloroplast targeting peptide (CTP) operably linked to a nucleotide sequence of interest, wherein the CTP is derived from Chlamydomonas sp. Plant transformation vectors, plants and plant cells having the CTP sequences are also encompassed, as well as variants and fragments of the CTP sequences. Methods for expressing a heterologous nucleotide sequence in a plant using the CTP sequences disclosed herein are also provided.

摘要翻译： 本发明提供了将多肽靶向高等植物的叶绿体的组合物和方法。 组合物包括具有编码与感兴趣的核苷酸序列可操作地连接的叶绿体靶向肽（CTP）的核苷酸序列的表达盒，其中CTP衍生自衣藻属。 还包括具有CTP序列的植物转化载体，植物和植物细胞，以及CTP序列的变体和片段。 还提供了使用本文公开的CTP序列在植物中表达异源核苷酸序列的方法。

公开(公告)号：US08299323B2

公开(公告)日：2012-10-30

申请号：US12701567

申请日：2010-02-07

申请人： Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck ,  Brian Carr

发明人： Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck ,  Brian Carr

IPC分类号： C12P21/02 ,  C12N15/82 ,  C12N15/31 ,  C12N1/21

CPC分类号： C12N15/8286 ,  C07K14/325 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, or the nucleotide sequence set forth in SEQ ID NO:1, as well as variants thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子及其变体。

公开(公告)号：US07834249B2

公开(公告)日：2010-11-16

申请号：US11943801

申请日：2007-11-21

申请人： Laura C. Schouten ,  Cheryl L. Peters ,  Brian Vande Berg

发明人： Laura C. Schouten ,  Cheryl L. Peters ,  Brian Vande Berg

IPC分类号： C12N15/52 ,  C12N15/82 ,  C12N1/21

CPC分类号： C12N15/8275 ,  C12N9/1085 ,  C12N9/1092

摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.

摘要翻译： 提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。 组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。 本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33， 或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

公开(公告)号：US07659376B2

公开(公告)日：2010-02-09

申请号：US12139408

申请日：2008-06-13

申请人： Philip E. Hammer ,  Nicholas B. Duck

发明人： Philip E. Hammer ,  Nicholas B. Duck

IPC分类号： C07K16/00

CPC分类号： C12N15/8275

摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules encoding glyphosate resistance proteins are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2 or the nucleotide sequence set forth in SEQ ID NO:1.

摘要翻译： 提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。 提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的植物，植物细胞，组织和种子。 特别地，提供编码抗草甘膦蛋白质的分离的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了包含编码SEQ ID NO：2所示的氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列的分离的核酸分子。

公开(公告)号：US20100005543A1

公开(公告)日：2010-01-07

申请号：US12491396

申请日：2009-06-25

申请人： Kimberly S. Sampson ,  Daniel John Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

发明人： Kimberly S. Sampson ,  Daniel John Tomso ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell

IPC分类号： C12N15/82 ,  C12N15/32 ,  C12N1/21 ,  C07K14/325 ,  C07K16/12 ,  A01N37/18 ,  C12P21/02 ,  A01H5/00 ,  C12N5/10 ,  A01H5/10 ,  A01P7/04

CPC分类号： C07K14/325 ,  A01N37/18 ,  C07K16/1278 ,  C12N15/8286 ,  Y02A40/162

摘要： Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:61-121 and 133-141, or the nucleotide sequence set forth in SEQ ID NO:1-60, 124-132, and 142-283, as well as variants and fragments thereof.

摘要翻译： 提供了赋予细菌，植物，植物细胞，组织和种子的杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中，用于在植物和细菌中转化和表达。 组合物还包括转化的细菌，植物，植物细胞，组织和种子。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：61-121和133-141所示的氨基酸序列的核苷酸序列或SEQ ID NO：1-60,124所示的核苷酸序列 -132和142-283，以及其变体和片段。

公开(公告)号：US20100004176A1

公开(公告)日：2010-01-07

申请号：US12497221

申请日：2009-07-02

申请人： Kimberly S. Sampson ,  Daniel J. Tomso ,  Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell ,  Volker Heinrichs

发明人： Kimberly S. Sampson ,  Daniel J. Tomso ,  Nadine Carozzi ,  Tracy Hargiss ,  Michael G. Koziel ,  Nicholas B. Duck ,  Shruti Agarwal ,  Brian McNulty ,  Chris Campbell ,  Volker Heinrichs

IPC分类号： A61K38/16 ,  C07H21/04 ,  C12N15/63 ,  C12N5/00 ,  C07K14/00 ,  C07K16/00

CPC分类号： C12N15/8286 ,  A01N37/18 ,  C07K14/32 ,  C07K14/325 ,  C07K16/1278 ,  Y02A40/162

摘要： Compositions and methods for conferring insecticidal activity to host cells are provided. Compositions comprising a coding sequence for a delta-endotoxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in host cells. Compositions also comprise transformed host cells. In particular, isolated delta-endotoxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:4, 5, 6, 13, or 14, or the nucleotide sequence set forth in SEQ ID NO:1, 2, 3, 11, or 12, as well as variants and fragments thereof.

摘要翻译： 提供了赋予宿主细胞杀虫活性的组合物和方法。 提供了包含δ-内毒素多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在宿主细胞中转化和表达。 组合物还包含转化的宿主细胞。 特别地，提供了分离的δ-内毒素核酸分子。 另外，包括对应于多核苷酸的氨基酸序列，以及与这些氨基酸序列特异性结合的抗体。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：4,5,6,13或14所示氨基酸序列的核苷酸序列或SEQ ID NO：1所示的核苷酸序列， 2,3,11或12，以及其变体和片段。

公开(公告)号：US20090241219A1

公开(公告)日：2009-09-24

申请号：US12423926

申请日：2009-04-15

申请人： Philip E. Hammer ,  Todd K. Hinson ,  Nicholas B. Duck

发明人： Philip E. Hammer ,  Todd K. Hinson ,  Nicholas B. Duck

IPC分类号： A01H5/00 ,  C12N15/82 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21 ,  C12N5/10 ,  C12N9/14 ,  C12P21/02

CPC分类号： C12N9/88 ,  C12N15/8275 ,  C12N15/8278

摘要： Compositions and methods for conferring herbicide resistance to plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a polypeptide that confers resistance or tolerance to glyphosate herbicides are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants. Compositions also comprise transformed plants, plant cells, tissues, and seeds. In particular, isolated nucleic acid molecules corresponding to glyphosate resistant nucleic acid sequences are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:3 or the nucleotide sequences set forth in SEQ ID NOS:1 and 2.

摘要翻译： 提供了赋予对植物，植物细胞，组织和种子的除草剂抗性的组合物和方法。 提供了包含赋予对草甘膦除草剂的抗性或耐受性的多肽的编码序列的组合物。 编码序列可用于DNA构建体或表达盒中用于在植物中转化和表达。 组合物还包括转化的植物，植物细胞，组织和种子。 特别地，提供了对应于草甘膦抗性核酸序列的分离的核酸分子。 另外，包括对应于多核苷酸的氨基酸序列。 特别地，本发明提供了分离的核酸分子，其包含编码SEQ ID NO：3所示的氨基酸序列的核苷酸序列或SEQ ID NO：1和2所示的核苷酸序列。