公开(公告)号：US10208092B2

公开(公告)日：2019-02-19

申请号：US15526895

申请日：2015-12-11

Applicant: GE HEALTHCARE BIOPROCESS R&D AB

Inventor： Gustav Rodrigo ,  Mats Ander ,  Tomas Bjorkman

IPC: C07K14/195 ,  C07K1/22 ,  C07K16/00

Abstract: The invention discloses kappa light chain-binding polypeptide comprising a mutated binding domain of Peptostreptococcus protein L, wherein at least one asparagine residue of a parental domain defined by, or having at least 95% or 98% sequence homology with, SEQ ID NO: 2-6 or 2 has been mutated to another amino acid residue which is not asparagine, proline or cysteine.

公开(公告)号：US10266563B2

公开(公告)日：2019-04-23

申请号：US15308327

申请日：2015-06-04

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Jean-Luc Maloisel ,  Ola Lind ,  Bjorn Noren ,  Ronnie Palmgren

IPC: C07K1/22 ,  C07K16/00 ,  C07K16/06 ,  C07K1/14

Abstract: The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.

公开(公告)号：US10654887B2

公开(公告)日：2020-05-19

申请号：US15282367

申请日：2016-09-30

Applicant: GE Healthcare BioProcess R&D AB

Inventor： Gustav Jose Rodrigo ,  Tomas Bjorkman ,  Mats Ander ,  Jesper Ulf Hansson

IPC: C07K1/22 ,  B01J20/26 ,  C07K16/00 ,  C07K14/31 ,  B01J20/32 ,  B01J20/285 ,  B01J20/286 ,  C07K16/12 ,  C07K17/10

Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.