公开(公告)号：US08742089B2

公开(公告)日：2014-06-03

申请号：US13601401

申请日：2012-08-31

Applicant: Gregory Duke ,  George Kemble ,  James Young ,  Zhaoti Wang

Inventor： Gregory Duke ,  George Kemble ,  James Young ,  Zhaoti Wang

IPC: C07H21/04 ,  C12N15/00 ,  C12N15/113

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  C12N9/1247 ,  C12N15/85 ,  C12N2510/02 ,  C12N2760/16134 ,  C12N2760/16234 ,  C12N2830/008 ,  C12Q1/70

Abstract: The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.

Abstract translation: 本发明提供了可用于在犬细胞如MDCK细胞中表达核酸序列的新型canine pol I调节核酸序列。 本发明进一步提供了包含这种核酸的表达载体和细胞以及使用这种核酸来制造流感病毒（包括感染性流感病毒）的方法。

公开(公告)号：US20120196371A1

公开(公告)日：2012-08-02

申请号：US13309498

申请日：2011-12-01

Applicant: George Kemble ,  Gregory Duke

Inventor： George Kemble ,  Gregory Duke

IPC: C12N15/64

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5254 ,  A61K2039/543 ,  C12N15/85 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/16162 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/16252 ,  C12N2760/16261 ,  C12N2840/20

Abstract: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.

Abstract translation: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外，本发明提供了生产能够在含胚鸡蛋和/或细胞（例如Vero和/或MDCK）中复制的流感病毒的方法，并进一步提供具有增强的复制特征的流感病毒。 此外，本发明包括一种改进的抢救方法，其中用本发明的质粒和载体对动物细胞（例如，SF Vero细胞）进行电穿孔。

公开(公告)号：US20090175907A1

公开(公告)日：2009-07-09

申请号：US12254131

申请日：2008-10-20

Applicant: Erich Hoffman ,  Hong Jin ,  Bin Lu ,  Gregory Duke ,  George Kemble ,  Zhongying Chen

Inventor： Erich Hoffman ,  Hong Jin ,  Bin Lu ,  Gregory Duke ,  George Kemble ,  Zhongying Chen

IPC: A61K39/145 ,  C12N7/01 ,  C12N15/74 ,  A61P31/16

CPC classification number: C12N15/86 ,  A61K2039/5254 ,  C07K14/005 ,  C12N7/00 ,  C12N15/85 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/16143 ,  C12N2760/16162 ,  C12N2760/16222 ,  C12N2760/16234 ,  C12N2760/16243 ,  C12N2760/16262 ,  C12N2840/20

Abstract: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.

Abstract translation: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外，本发明提供了生产能够在含胚鸡蛋和/或细胞（例如Vero和/或MDCK）中复制的流感病毒的方法，并进一步提供具有增强的复制特征的流感病毒。 还提供了生产在例如25℃下有效复制的冷适应（ca）流感病毒的方法（和包含其的免疫原性组合物）。

公开(公告)号：US07465456B2

公开(公告)日：2008-12-16

申请号：US11133345

申请日：2005-05-20

Applicant: Erich Hoffmann ,  Hong Jin ,  Bin Lu ,  Gregory Duke ,  George Kemble ,  Zhongying Chen

Inventor： Erich Hoffmann ,  Hong Jin ,  Bin Lu ,  Gregory Duke ,  George Kemble ,  Zhongying Chen

IPC: A61K39/145 ,  C12Q1/70 ,  C12P19/34

CPC classification number: C12N15/86 ,  A61K2039/5254 ,  C07K14/005 ,  C12N7/00 ,  C12N15/85 ,  C12N2760/16122 ,  C12N2760/16134 ,  C12N2760/16143 ,  C12N2760/16162 ,  C12N2760/16222 ,  C12N2760/16234 ,  C12N2760/16243 ,  C12N2760/16262 ,  C12N2840/20

Abstract: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. A method of producing a cold adapted (ca) influenza virus that replicates efficiently at, e.g., 25° C. (and immunogenic compositions comprising the same) is also provided.

Abstract translation: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外，本发明提供了生产能够在含胚鸡蛋和/或细胞（例如Vero和/或MDCK）中复制的流感病毒的方法，并进一步提供具有增强的复制特征的流感病毒。 还提供了生产在例如25℃下有效复制的冷适应（ca）流感病毒的方法（和包含其的免疫原性组合物）。

公开(公告)号：US20050158342A1

公开(公告)日：2005-07-21

申请号：US11018624

申请日：2004-12-22

Applicant: George Kemble ,  Gregory Duke

Inventor： George Kemble ,  Gregory Duke

IPC: A61K35/76 ,  A61K39/00 ,  A61K39/12 ,  A61K39/145 ,  C12N7/02 ,  C12N15/85

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5254 ,  A61K2039/543 ,  C12N15/85 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/16162 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/16252 ,  C12N2760/16261 ,  C12N2840/20

Abstract: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.

Abstract translation: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外，本发明提供了生产能够在含胚鸡蛋和/或细胞（例如Vero和/或MDCK）中复制的流感病毒的方法，并进一步提供具有增强的复制特征的流感病毒。 此外，本发明包括一种改进的抢救方法，其中用本发明的质粒和载体对动物细胞（例如，SF Vero细胞）进行电穿孔。

公开(公告)号：US08093033B2

公开(公告)日：2012-01-10

申请号：US12336158

申请日：2008-12-16

Applicant: George Kemble ,  Gregory Duke

Inventor： George Kemble ,  Gregory Duke

IPC: C12N7/00 ,  C12N5/07 ,  A61K39/145

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  A61K2039/5254 ,  A61K2039/543 ,  C12N15/85 ,  C12N2760/16134 ,  C12N2760/16151 ,  C12N2760/16152 ,  C12N2760/16162 ,  C12N2760/16234 ,  C12N2760/16251 ,  C12N2760/16252 ,  C12N2760/16261 ,  C12N2840/20

Abstract: Vectors and methods for the production of influenza viruses suitable as recombinant influenza vaccines in cell culture are provided. Bi-directional expression vectors for use in a multi-plasmid influenza virus expression system are provided. Additionally, the invention provides methods of producing influenza viruses with enhanced ability to replicate in embryonated chicken eggs and/or cells (e.g., Vero and/or MDCK) and further provides influenza viruses with enhanced replication characteristics. In addition, the present invention includes an improved method of rescue, wherein animal cells (e.g., SF Vero cells) are electroporated with plasmids and vectors of the invention.

Abstract translation: 提供了用于在细胞培养中生产适合作为重组流感疫苗的流感病毒的载体和方法。 提供了用于多质粒流感病毒表达系统的双向表达载体。 另外，本发明提供了生产能够在含胚鸡蛋和/或细胞（例如Vero和/或MDCK）中复制的流感病毒的方法，并进一步提供具有增强的复制特征的流感病毒。 此外，本发明包括一种改进的抢救方法，其中用本发明的质粒和载体对动物细胞（例如，SF Vero细胞）进行电穿孔。

公开(公告)号：US20070292455A1

公开(公告)日：2007-12-20

申请号：US11614141

申请日：2006-12-21

Applicant: George Kemble ,  Gregory Duke ,  Richard Spaete

Inventor： George Kemble ,  Gregory Duke ,  Richard Spaete

IPC: A61K39/245 ,  A61P37/04 ,  C12N7/00

CPC classification number: C12N7/00 ,  C12N2710/16162

Abstract: A method is provided for attenuating a cytomegalovirus comprising functionally disrupting an open reading frame of a Toledo genome region or its homolog and making chimeric CMV virus genomes.

Abstract translation: 提供了一种减毒巨细胞病毒的方法，其包括功能性地破坏托莱多基因组区域的开放阅读框或其同源物并制备嵌合CMV病毒基因组。

公开(公告)号：US20120329150A1

公开(公告)日：2012-12-27

申请号：US13601401

申请日：2012-08-31

Applicant: Gregory Duke ,  George Kemble ,  James Young ,  Zhaoti Wang

Inventor： Gregory Duke ,  George Kemble ,  James Young ,  Zhaoti Wang

IPC: C12N15/113 ,  C12N5/10 ,  C12N1/19 ,  C12N15/63 ,  C12N1/21

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  C12N9/1247 ,  C12N15/85 ,  C12N2510/02 ,  C12N2760/16134 ,  C12N2760/16234 ,  C12N2830/008 ,  C12Q1/70

Abstract: The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.

Abstract translation: 本发明提供了可用于在犬细胞如MDCK细胞中表达核酸序列的新型canine pol I调节核酸序列。 本发明进一步提供了包含这种核酸的表达载体和细胞以及使用这种核酸来制造流感病毒（包括感染性流感病毒）的方法。

公开(公告)号：US20100203641A1

公开(公告)日：2010-08-12

申请号：US12706994

申请日：2010-02-17

Applicant: Gregory DUKE ,  George KEMBLE ,  James YOUNG ,  Zhaoti WANG

Inventor： Gregory DUKE ,  George KEMBLE ,  James YOUNG ,  Zhaoti WANG

IPC: C12N15/00 ,  C07H21/00 ,  C12N15/63 ,  C12N7/00 ,  C12N5/07

CPC classification number: C12N7/00 ,  A61K39/12 ,  A61K39/145 ,  C12N9/1247 ,  C12N15/85 ,  C12N2510/02 ,  C12N2760/16134 ,  C12N2760/16234 ,  C12N2830/008 ,  C12Q1/70

Abstract: The present invention provides novel canine pol I regulatory nucleic acid sequences useful for the expression of nucleic acid sequences in canine cells such as MDCK cells. The invention further provides expression vectors and cells comprising such nucleic acids as well as methods of using such nucleic acids to make influenza viruses, including infectious influenza viruses.

Abstract translation: 本发明提供了可用于在犬细胞如MDCK细胞中表达核酸序列的新型canine pol I调节核酸序列。 本发明进一步提供了包含这种核酸的表达载体和细胞以及使用这种核酸来制造流感病毒（包括感染性流感病毒）的方法。

公开(公告)号：US20090297555A1

公开(公告)日：2009-12-03

申请号：US12495241

申请日：2009-06-30

Applicant: GEORGE KEMBLE ,  HARRY GREENBERG ,  GREGORY DUKE

Inventor： GEORGE KEMBLE ,  HARRY GREENBERG ,  GREGORY DUKE

IPC: A61K39/245 ,  C12N7/01 ,  A61P31/22

CPC classification number: A61K39/245 ,  A61K39/12 ,  A61K2039/5256 ,  A61K2039/53 ,  C12N2710/16134 ,  C12N2710/16143 ,  C12N2770/24234 ,  Y02A50/41

Abstract: The present invention relates to recombinant HCMV (human cytomegalovirus) expressing a pp65 polypeptide or fragment thereof fused to a heterologous or non-native polypeptide, in particular immunogenic and/or antigenic polypeptides. In particular, the heterologous gene products include antigenic or immunogenic polypeptides from a variety of pathogens, cellular genes, tumor antigens, and viruses. The recombinant viruses may advantageously be used in vaccine formulations including vaccines against a broad range of pathogens and antigens.

Abstract translation: 本发明涉及表达与异源或非天然多肽，特别是免疫原性和/或抗原性多肽融合的pp65多肽或其片段的重组HCMV（人巨细胞病毒）。 特别地，异源基因产物包括来自多种病原体，细胞基因，肿瘤抗原和病毒的抗原性或免疫原性多肽。 重组病毒可有利地用于疫苗制剂，包括针对各种病原体和抗原的疫苗。