公开(公告)号：US20070293394A1

公开(公告)日：2007-12-20

申请号：US10595755

申请日：2004-11-10

Applicant: Robert-Matthias Leiser ,  Dimitri Kapoustine ,  Vitali Zubov ,  Hamlet Balayan ,  Lutz Plobner ,  Gottfried Brem

Inventor： Robert-Matthias Leiser ,  Dimitri Kapoustine ,  Vitali Zubov ,  Hamlet Balayan ,  Lutz Plobner ,  Gottfried Brem

IPC: B01J20/32 ,  B01D15/08 ,  B01J20/10 ,  B01J20/26

CPC classification number: B01J20/28092 ,  B01J20/103 ,  B01J20/26 ,  B01J20/262 ,  B01J20/28016 ,  B01J20/28033 ,  B01J20/28035 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3268 ,  B01J20/3272 ,  G01N2030/525 ,  Y10S502/509

Abstract: A composite polymer-coated sorbent with a bidisperse or oligodisperse distribution of pore sizes and having an at least partial coating on its surface, which coating comprises essentially polyanilines or derivatives of polyanilines and use thereof for the simultaneous separation and desalting of bio-macromolecules.

Abstract translation: 一种复合聚合物涂覆的吸附剂，其具有孔尺寸的双分散或小分散分布，并且在其表面上具有至少部分涂层，该涂层基本上包含聚苯胺或聚苯胺衍生物及其用于同时分离和脱盐生物大分子的用途。

公开(公告)号：US07772152B2

公开(公告)日：2010-08-10

申请号：US10595755

申请日：2004-11-10

Applicant: Robert-Matthias Leiser ,  Dimitri Valerjewich Kapoustine ,  Vitali Pavlovich Zubov ,  Hamlet Balayan ,  Lutz Plobner ,  Gottfried Brem

Inventor： Robert-Matthias Leiser ,  Dimitri Valerjewich Kapoustine ,  Vitali Pavlovich Zubov ,  Hamlet Balayan ,  Lutz Plobner ,  Gottfried Brem

IPC: B01J20/22

CPC classification number: B01J20/28092 ,  B01J20/103 ,  B01J20/26 ,  B01J20/262 ,  B01J20/28016 ,  B01J20/28033 ,  B01J20/28035 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3268 ,  B01J20/3272 ,  G01N2030/525 ,  Y10S502/509

Abstract: A composite polymer-coated sorbent with a bidisperse or oligodisperse distribution of pore sizes and having an at least partial coating on its surface, which coating comprises essentially polyanilines or derivatives of polyanilines and use thereof for the simultaneous separation and desalting of bio-macromolecules.

Abstract translation: 一种复合聚合物涂覆的吸附剂，其具有孔尺寸的双分散或小分散分布，并且在其表面上具有至少部分涂层，该涂层基本上包含聚苯胺或聚苯胺衍生物及其用于同时分离和脱盐生物大分子的用途。

公开(公告)号：US20080154029A1

公开(公告)日：2008-06-26

申请号：US11547011

申请日：2005-04-01

Applicant: Hamlet Balayan ,  Robert-Matthias Leiser ,  Lutz Plobner ,  Leonti E. Tkachenko ,  Gottfried Brem

Inventor： Hamlet Balayan ,  Robert-Matthias Leiser ,  Lutz Plobner ,  Leonti E. Tkachenko ,  Gottfried Brem

IPC: C07H21/00 ,  C08F216/12 ,  C08F220/04 ,  B01J20/26 ,  B01D15/08 ,  C08F220/10 ,  C08F212/00

CPC classification number: C12N15/101 ,  B01J20/103 ,  B01J20/261 ,  B01J20/265 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/28088 ,  B01J20/283 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/3278 ,  C08F212/08 ,  C08F212/14 ,  C08F292/00 ,  C08F2/44 ,  C08F212/36

Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.

Abstract translation: 本发明涉及用于分离和纯化生物聚合物，特别是核酸的吸附剂材料，其具有基本上用包含芳族单体和交联化合物的共聚物涂层基本上改性的固体载体以及优选通过乙烯基氯硅烷连接到载体上的不饱和酯或醚的固体载体。 使用这些材料来分离核酸，特别是从不同生物来源的裂解物中分离DNA的一步法是本发明的一个目的，以及至少部分填充有吸附剂材料的色谱柱或筒， 本发明涉及一种包含本发明的吸附剂材料的膜状装置，以及包含本发明的吸附剂材料或包装在色谱装置中以及执行样品制备所需的其它装置的试剂盒。

公开(公告)号：US20110186519A1

公开(公告)日：2011-08-04

申请号：US12830676

申请日：2010-07-06

Applicant: Hamlet Balayan ,  Robert-Matthias Leiser ,  Lutz Plobner ,  Leonti E. Tkachenko ,  Gottfried Brem

Inventor： Hamlet Balayan ,  Robert-Matthias Leiser ,  Lutz Plobner ,  Leonti E. Tkachenko ,  Gottfried Brem

IPC: B01D15/00

CPC classification number: C12N15/101 ,  B01J20/103 ,  B01J20/261 ,  B01J20/265 ,  B01J20/28059 ,  B01J20/28061 ,  B01J20/28083 ,  B01J20/28085 ,  B01J20/28088 ,  B01J20/283 ,  B01J20/289 ,  B01J20/3204 ,  B01J20/3219 ,  B01J20/3278 ,  C08F212/08 ,  C08F212/14 ,  C08F292/00 ,  C08F2/44 ,  C08F212/36

Abstract: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.

Abstract translation: 本发明涉及用于分离和纯化生物聚合物，特别是核酸的吸附剂材料，其具有基本上用包含芳族单体和交联化合物的共聚物涂层基本上改性的固体载体以及优选通过乙烯基氯硅烷连接到载体上的不饱和酯或醚的固体载体。 使用这些材料来分离核酸，特别是从不同生物来源的裂解物中分离DNA的一步法是本发明的一个目的，以及至少部分填充有吸附剂材料的色谱柱或筒， 本发明涉及一种包含本发明的吸附剂材料的膜状装置，以及包含本发明的吸附剂材料或包装在色谱装置中以及执行样品制备所需的其它装置的试剂盒。

公开(公告)号：US20070082354A1

公开(公告)日：2007-04-12

申请号：US11535333

申请日：2006-09-26

Applicant: Robert-Matthias Leiser ,  Hamlet Balayan ,  Nina Fritzemeier ,  Gottfried Brem

Inventor： Robert-Matthias Leiser ,  Hamlet Balayan ,  Nina Fritzemeier ,  Gottfried Brem

IPC: C12Q1/68 ,  C07H21/02 ,  C12N1/08

CPC classification number: C12N15/1003 ,  C12N15/101

Abstract: A method for isolating RNA from RNA containing samples wherein the RNA containing sample is treated with at least one DNase and/or another enzyme like proteases or collagenases for purification of RNA in presence of a complex of ribonucleosides and the oxovanadyl ion which is capable of inhibiting RNases present in the RNA containing samples and removing the complex of ribonucleosides and the oxovanadyl ion prior to down-stream processing of the RNA of the RNA containing sample by contacting the sample of the forgoing step with a chelating agent immobilized to a support, the chelating agent having sufficient affinity to the complex of ribonucleosides and the oxovanadyl ion so that it binds to the chemical entity and the RNA containing sample is freed from the inhibitor.

Abstract translation: 从含有RNA的样品中分离RNA的方法，其中含有RNA的样品用至少一种DNA酶和/或其它酶如蛋白酶或胶原酶处理以在核糖核苷和能够抑制的核糖核苷和氧杂壬二酰离子的复合物存在下纯化RNA 存在于含有RNA的样品中的RNA酶，并且在通过将前述步骤的样品与固定在载体上的螯合剂接触的情况下，在含RNA样品的RNA的下游加工之前去除核糖核苷和氧杂壬酰基离子的复合物，螯合 对核糖核苷和氧杂壬酰离子的复合物具有足够亲和力的试剂，使其与化学实体结合并且含有RNA的样品与抑制剂无关。

公开(公告)号：US20060243658A1

公开(公告)日：2006-11-02

申请号：US10534031

申请日：2003-11-10

Applicant: Vitali Zubov ,  Lutz Plobner ,  Dimitri Kapoustine ,  Hamlet Balayan ,  Makhmud Muydinov ,  Gottfried Brem ,  Robert-Matthias Leiser

Inventor： Vitali Zubov ,  Lutz Plobner ,  Dimitri Kapoustine ,  Hamlet Balayan ,  Makhmud Muydinov ,  Gottfried Brem ,  Robert-Matthias Leiser

IPC: B01D39/00

CPC classification number: G01N30/482 ,  B01J20/28026 ,  B01J20/28035 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/327 ,  B01J20/3276 ,  B01J20/328 ,  B01J2220/54 ,  Y10T428/2991 ,  Y10T428/2998

Abstract: The present invention relates to a sorbent material having a solid support substantially modified with a fluorinated polymer coating which is covalently attached to the support and the fluorinated polymer coating is containing at least one functional group, methods of obtaining the sorbent material, the use of these materials for separation of substances, a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, a device comprising the sorbent material of the invention in loose form as well as a miniaturized device comprising the sorbent material.

Abstract translation: 本发明涉及一种吸附剂材料，其具有基本上被氟化聚合物涂层改性的固体载体，该涂层共价连接到载体上，氟化聚合物涂层含有至少一个官能团，获得吸附剂材料的方法，使用这些 用于分离物质的材料，至少部分填充有本发明的吸附剂材料的色谱柱或筒，包括本发明的吸附剂材料的膜状装置，包含松散形式的本发明吸附材料的装置 作为包括吸附剂材料的小型化装置。