PROCESS FOR MANUFACTURING A COMPOSITE SORBENT MATERIAL FOR CHROMATOGRAPHICAL SEPARATION OF BIOPOLYMERS
    8.
    发明申请
    PROCESS FOR MANUFACTURING A COMPOSITE SORBENT MATERIAL FOR CHROMATOGRAPHICAL SEPARATION OF BIOPOLYMERS 审中-公开
    制备用于生物聚合物色谱分离的复合吸附材料的方法

    公开(公告)号:US20160097048A1

    公开(公告)日:2016-04-07

    申请号:US14966269

    申请日:2015-12-11

    申请人: NExtTec GmbH

    IPC分类号: C12N15/10 B01J20/26

    摘要: The present invention relates to a sorbent material for separation and purification of biopolymers, particularly nucleic acids, having a solid support substantially modified with a copolymer coating comprising aromatic monomers and crosslinking compounds and unsaturated esters or ethers preferably attached to the support via a vinylchlorsilane. The use of these materials for separation of nucleic acids, particularly a one-step isolation of DNA from lysates of different biological sources, is an object of the invention as well as a chromatographic column or cartridge at least partially filled with the sorbent material of the invention, a membrane-like device comprising the sorbent material of the invention, and a kit comprising the sorbent material of the invention in bulk or packed in chromatographic devices as well as other devices necessary for performing sample preparations.

    摘要翻译: 本发明涉及用于分离和纯化生物聚合物,特别是核酸的吸附剂材料,其具有基本上用包含芳族单体和交联化合物的共聚物涂层基本上改性的固体载体以及优选通过乙烯基氯硅烷连接到载体上的不饱和酯或醚的固体载体。 使用这些材料来分离核酸,特别是从不同生物来源的裂解物中分离DNA的一步法是本发明的一个目的,以及至少部分填充有吸附剂材料的色谱柱或筒, 本发明涉及一种包含本发明的吸附剂材料的膜状装置,以及包含本发明的吸附剂材料或包装在色谱装置中以及执行样品制备所需的其它装置的试剂盒。