利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

9 条记录 (耗时 0.018 秒)

    Metalloligands for cleaving nucleic acids
    6.
    发明授权
    Metalloligands for cleaving nucleic acids 失效
    用于切割核酸的金属配体

    公开(公告)号：US06403777B1

    公开(公告)日：2002-06-11

    申请号：US09348225

    申请日：1999-07-06

    申请人： James A. Cowan

    发明人： James A. Cowan

    IPC分类号： C07H1500

    CPC分类号： C07H23/00

    摘要： Transition metal complexes, referred to hereinafter as “metalloligands”, that catalyze the degradation of DNA and the cleavage of RNA at select sites are provided. In one embodiment, the metalloligand has the following structure: wherein R1 is an amino group, i.e. an NH, or an alkylamino group comprising 1 or 2 carbon atoms; wherein R2 is selected from the group consisting of an amino group, a hydroxyl group, i.e., O(H), an alkylamino group comprising 1 or 2 carbon atoms; and an alkylhydroxyl group comprising 1 or 2 carbon atoms; wherein J is a ligand which comprises at least one carbon-containing five-membered or six-membered ring structure; and wherein M is a transition metal ion which is bound via coordinate bonds to R1 and R2. In another embodiment the metalloligand has the following structure: wherein R1′ and R1″ are the same or different and wherein R1′ and R1″ are an amino group or an alkylamino group comprising 1 or 2 carbon atoms; wherein R2′ and R2″ are the same or different and wherein R2′ and R2″ are selected from the group consisting of an amino group, a hydroxyl group, an alkylamino group comprising 1 or 2 carbon atoms, and an alkylhydroxyl group comprising one or two carbon atoms; wherein J′ and J″ are the same or different and wherein J′ and J″ are ligands which comprise at least one carbon-containing five-membered or six-membered ring structure; and wherein M is a transition metal ion which is bound via coordinate bonds to R1′, R1″, R2′ and R2″. Methods of cleaving nucleic acids using the metalloligands are also provided.

    摘要翻译： 提供了以下称为“金属配体”的过渡金属络合物，其催化DNA的降解和选择位点处的RNA的切割。 在一个实施方案中，金属配体具有以下结构：其中R 1是氨基，即NH，或包含1或2个碳原子的烷基氨基; 其中R2选自氨基，羟基，即O（H），含有1或2个碳原子的烷基氨基; 和含有1或2个碳原子的烷基羟基; 其中J是包含至少一个含碳的五元或六元环结构的配体; 并且其中M是通过配位键与R 1和R 2结合的过渡金属离子。在另一个实施方案中，金属配体具有以下结构：其中R1'和R1“相同或不同，并且其中R1'和R1” 氨基或含有1或2个碳原子的烷基氨基; 其中R2'和R2“相同或不同，并且其中R2'和R2”选自氨基，羟基，包含1或2个碳原子的烷基氨基，和烷基羟基，包括 一个或两个碳原子; 其中J'和J“相同或不同，并且其中J'和J”是包含至少一个含碳五元或六元环结构的配体; 并且其中M是通过配位键与R1'，R1“，R2'和R2”结合的过渡金属离子。还提供了使用金属配体切割核酸的方法。

    Freight container ventilation system
    9.
    发明授权
    Freight container ventilation system 失效
    货运集装箱通风系统

    公开(公告)号：US6138578A

    公开(公告)日：2000-10-31

    申请号：US024810

    申请日：1998-02-17

    申请人： James A. Cowan, Jr. ,  Michael D. Gaikowski ,  John K. Deveikis ,  Richard J. Kloster

    发明人： James A. Cowan, Jr. ,  Michael D. Gaikowski ,  John K. Deveikis ,  Richard J. Kloster

    IPC分类号： B61D27/00 ,  B61D11/00 ,  B61D3/00

    CPC分类号： B61D27/009

    摘要： A ventilated transportable freight container is disclosed. The container is ventilated by natural convection through at least one lower side vent comprising a lower plenum chamber for distributing air into the container and restricting entry of moisture in the form of rain, sleet or snow into the container and at least one upper top vent, including a plenum chamber. The upper top vent or vents may be formed in the sidewall of the enclosure or in the roof of the enclosure depending upon the structure of the container and the particular application. The use of one or more upper top vents enhances circulation of air by natural convection. The invention also incorporates sidewall and endwall spacers extending from the sidewalls and endwalls into the enclosure to provide passageways for the circulation of air to ventilate the enclosure and a path for condensation drainage. In one embodiment of the invention, the transportable freight container of present invention comprises a rail freight car. In another embodiment of the invention, at least one of the lower plenum chambers of the container extends lower than the floor of the enclosure to allow drainage from the enclosure. The container may also incorporate a layer of insulating material on the interior surface of the roof of the container to inhibit condensation of moisture. The freight container of the present invention is particularly adapted to the transport of baled material, such as pulp, which is typically shipped or transported in bales of approximately one cubic yard.

    摘要翻译： 公开了一种通风运输货物集装箱。 容器通过自然对流通过至少一个下侧通风口通风，该至少一个下侧通风口包括用于将空气分配到容器中的下部增压室，并限制雨水，雨夹雪或雪形式的水分进入容器和至少一个上部顶部排气口， 包括一个集会厅。 根据容器的结构和特定应用，顶部顶部通风孔或通风口可以形成在外壳的侧壁或外壳的顶部中。 使用一个或多个上部顶部通风口可以通过自然对流增强空气的循环。 本发明还包括从侧壁和端壁延伸到外壳中的侧壁和端壁间隔件，以提供用于循环空气以使外壳通风和冷凝排水路径的通道。 在本发明的一个实施例中，本发明的可运输货物集装箱包括铁路货车。 在本发明的另一个实施例中，容器的下部增压室中的至少一个延伸低于外壳的底板，以允许从外壳排出。 容器还可以在容器的顶部的内表面上并入绝缘材料层，以抑制水分的冷凝。 本发明的货物容器特别适用于运输包装材料，例如纸浆，其通常以大约一立方码包的方式运输或运输。