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    Covalently attached collagen VI for cell attachment and proliferation
    1.
    发明申请
    Covalently attached collagen VI for cell attachment and proliferation 审中-公开
    共价连接胶原蛋白VI用于细胞附着和增殖

    公开(公告)号：US20060105455A1

    公开(公告)日：2006-05-18

    申请号：US11322637

    申请日：2005-12-30

    申请人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    发明人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    IPC分类号： C12N5/08

    CPC分类号： G01N33/54393 ,  C12N5/0068 ,  C12N2500/90 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/80

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

    摘要翻译： 用于细胞培养物的表面包括与CAR材料结合的载体，并且结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体） ），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。 此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。 该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系 ）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。 还公开了包含表面和附加试剂的试剂盒。

    High throughput method to identify ligands for cell attachment
    2.
    发明申请
    High throughput method to identify ligands for cell attachment 审中-公开
    用于鉴定细胞附着配体的高通量方法

    公开(公告)号：US20050059083A1

    公开(公告)日：2005-03-17

    申请号：US10662640

    申请日：2003-09-15

    申请人： Andrea Liebmann-Vinson ,  Jonathan Rowley ,  Chris Bodily ,  Mohammad Heidaran

    发明人： Andrea Liebmann-Vinson ,  Jonathan Rowley ,  Chris Bodily ,  Mohammad Heidaran

    IPC分类号： G01N33/50 ,  G01N33/543 ,  G01N33/53

    CPC分类号： G01N33/5011 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5029 ,  G01N33/54393

    摘要： A high throughput method is provided for identifying agents capable of producing a desired biological response in whole cells. The method includes the steps of providing receptacles having a culture surface; placing different mixtures of single agents into selective ones of the receptacles according to a statistical design; and immobilizing the mixtures of single agents to the culture surface. The method further includes contacting the immobilized agents with the whole cells; and acquiring data which is indicative of a desired biological response in the contacted cells. The method also includes using statistical modeling of the acquired data to determine which mixtures of single agents and/or which single agents in these mixtures are effective in producing the desired biological response in the contacted cells.

    摘要翻译： 提供了一种高通量方法，用于鉴定能够在全细胞中产生所需生物反应的试剂。 该方法包括提供具有培养表面的容器的步骤; 根据统计设计将单一试剂的不同混合物置于选择性容器中; 并将单一试剂的混合物固定到培养表面。 该方法还包括使固定化的试剂与整个细胞接触; 并且获取指示所接触细胞中所需生物反应的数据。 该方法还包括使用获取的数据的统计建模来确定单个试剂的哪些混合物和/或这些混合物中的哪一种试剂在接触的细胞中产生所需的生物反应是有效的。

    Covalently attached collagen VI for cell attachment and proliferation
    3.
    发明申请
    Covalently attached collagen VI for cell attachment and proliferation 审中-公开
    共价连接胶原蛋白VI用于细胞附着和增殖

    公开(公告)号：US20050058687A1

    公开(公告)日：2005-03-17

    申请号：US10660781

    申请日：2003-09-12

    申请人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    发明人： Richard Guarino ,  Jonathan Rowley ,  Andrea Liebmann-Vinson ,  John Hemperly ,  Mohammad Heidaran ,  Sharon Presnell

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/08 ,  G01N33/543

    CPC分类号： G01N33/54393 ,  C12N5/0068 ,  C12N2500/90 ,  C12N2533/52 ,  C12N2533/54 ,  C12N2533/80

    摘要： Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

    摘要翻译： 用于细胞培养物的表面包括与CAR材料结合的载体，并结合CAR材料，胶原VI或其生物活性片段或变体，以及任选的一种或多种其它ECM蛋白（或其片段或变体） ），例如弹性蛋白，纤连蛋白，玻连蛋白，腱生蛋白，层粘连蛋白，内含子，聚集蛋白聚糖，核心蛋白聚糖，胶原I，胶原III和胶原IV。 此外，任选存在于表面上的是一种或多种聚阳离子聚合物，例如聚-D-赖氨酸或聚-D-鸟氨酸。 该表面用于细胞培养以促进许多不同细胞类型的细胞附着，存活和/或增殖，例如（a）肝细胞（例如，HepG2肿瘤细胞和新发现的大鼠肝上皮干细胞系 ）（b）成骨细胞，例如鼠细胞系MC3T3细胞系和（c）原代骨髓细胞。 还公开了包含表面和附加试剂的试剂盒。

    AUTOMATED FILLING OF FLEXIBLE CRYOGENIC STORAGE BAGS WITH THERAPEUTIC CELLS
    4.
    发明申请
    AUTOMATED FILLING OF FLEXIBLE CRYOGENIC STORAGE BAGS WITH THERAPEUTIC CELLS 审中-公开
    具有治疗细胞的柔性低温储存袋的自动填充

    公开(公告)号：US20110287534A1

    公开(公告)日：2011-11-24

    申请号：US13099963

    申请日：2011-05-03

    申请人： Jonathan Rowley ,  Patrick Newsom

    发明人： Jonathan Rowley ,  Patrick Newsom

    IPC分类号： C12N5/071 ,  C12M3/00

    CPC分类号： A01N1/0268

    摘要： An apparatus and processes for aseptically dispensing live mammalian cells into sterile, flexible bags in a non-sterile atmosphere. The method includes the steps of: providing the cells suspended in a liquid; providing a plurality of sterile flexible bags fluidly connected to a main line by a plurality of branch lines of sterile flexible tubing; evacuating air from the flexible bags by applying a vacuum to the open end of the main line; preventing fluid flow through all branch lines except that of one bag to be filled; dispensing a desired volume of cell suspension into the open end of the main line; and introducing sufficient sterile purging gas under pressure into open end of the main line to drive into the bag any of the dispensed volume remaining in the main line or branch line of filled bag. Cells can be cryogenically preserved in the filled bags.

    摘要翻译： 用于在非无菌气氛中将活的哺乳动物细胞无菌分配到无菌的柔性袋中的装置和方法。 该方法包括以下步骤：提供悬浮在液体中的细胞; 提供多个无菌柔性袋，其通过多条无菌柔性管的分支管线流体地连接到主线; 通过向主线的开口端施加真空从空气中排出空气; 防止流体通过所有分支管线，除了一个待填充的袋子; 将所需体积的细胞悬浮液分配到主线的开口端; 并将足够的无菌吹扫气体在压力下引入主管线的开口端以驱动残留在填充袋的主线或分支管线中的任何分配体积。 细胞可以被低温保存在填充的袋子中。

    HIGH YIELD METHOD AND APPARATUS FOR VOLUME REDUCTION AND WASHING OF THERAPEUTIC CELLS USING TANGENTIAL FLOW FILTRATION
    5.
    发明申请
    HIGH YIELD METHOD AND APPARATUS FOR VOLUME REDUCTION AND WASHING OF THERAPEUTIC CELLS USING TANGENTIAL FLOW FILTRATION 审中-公开
    高效液相色谱方法和装置减少和洗涤治疗细胞使用流量过滤

    公开(公告)号：US20120294836A1

    公开(公告)日：2012-11-22

    申请号：US13552834

    申请日：2012-07-19

    申请人： Jonathan ROWLEY ,  Jacob PATTASSERIL ,  Ali MOHAMED

    发明人： Jonathan ROWLEY ,  Jacob PATTASSERIL ,  Ali MOHAMED

    IPC分类号： A61K35/12 ,  C12Q1/02 ,  C12N5/071

    CPC分类号： B01D61/145

    摘要： The present invention provides processes for aseptically processing live mammalian cells in an aqueous medium to produce a cell suspension having a cell density of at least about 10 million cells/mL and cell viability of at least about 90%. These methods comprise a step of reducing the volume of the medium using a tangential flow filter (TFF) having a pore size of greater than 0.1 micron, during which step the trans-membrane pressure (TMP) is maintained at less than about 3 psi and the shear rate is maintained at less than about 4000 sec−1. The invention also provides a complete process for large scale manufacturing mammalian cells for use in a therapeutic composition, and scalable, fully disposable systems for carrying out the process, using readily available disposables and pumps.

    摘要翻译： 本发明提供了在水性介质中无菌处理活的哺乳动物细胞以产生细胞密度为至少约1000万个细胞/ mL并且细胞存活率为至少约90％的细胞悬浮液的方法。 这些方法包括使用孔径大于0.1微米的切向流过滤器（TFF）来减小介质的体积的步骤，在此步骤期间，跨膜压力（TMP）保持在小于约3psi，以及 剪切速率保持在小于约4000秒-1。 本发明还提供了用于大规模生产用于治疗组合物的哺乳动物细胞的完整方法，以及使用易于获得的一次性物品和泵来执行该方法的可伸缩的完全一次性系统。