-
公开(公告)号：US5683903A
公开(公告)日：1997-11-04
申请号：US453117
申请日：1995-05-30
IPC分类号： G01N33/566 , C07K14/705 , C12N5/071 , C12N5/10 , C12N11/00 , C12N15/09 , C12N15/85 , C12P21/02 , C12R1/91 , G01N33/50 , C12N5/00
CPC分类号： G01N33/502 , C07K14/705 , C12N11/00 , C12N15/85 , C12N5/0686 , G01N33/5008 , C12N2501/50 , C12N2503/00 , C12N2510/00
摘要： Attachment enhanced human embryonic kidney cells, 293, are provided. These cells have been modified to contain a selected mammalian scavenger gene, which has been found to improve the ability of these cells to attach in culture. The improved cells of the invention are useful in assays in which the unmodified 293 cells could be used.
摘要翻译： 提供附着增强的人胚胎肾细胞293。 已经将这些细胞修饰成含有选择的哺乳动物清道夫基因，其被发现可以提高这些细胞附着在培养物中的能力。 本发明的改进的细胞可用于其中可以使用未修饰的293细胞的测定中。
-
公开(公告)号：US5435730A
公开(公告)日：1995-07-25
申请号：US99979
申请日：1993-07-30
摘要： A recombinant DNA molecule comprising the Streptomyces gal operon galK gene; galE gene; galT gene; P1 promoter; P2 promoter; P2 promoter expression unit; P1 promoter regulated region; or the entire Streptomyces gal operon.
摘要翻译： 包含链霉菌gal操纵子galK基因的重组DNA分子; galE基因; galT基因; P1启动子; P2启动子; P2启动子表达单位; P1启动子调控区; 或整个Streptomyces gal操纵子。
-
公开(公告)号：US06388055B1
公开(公告)日：2002-05-14
申请号：US08724984
申请日：1996-10-03
申请人： Derk J. Bergsma , Mary E. Brawner , Usman Shabon
发明人： Derk J. Bergsma , Mary E. Brawner , Usman Shabon
IPC分类号： C07K1400
CPC分类号： C07K14/7158 , A01K2217/05 , A01K2217/075 , A61K38/00
摘要： Mouse CC-CKR5 polypeptides and DNA (RNA) encoding such mouse CC-CKR5 and a procedure for producing such polypeptides by recombinant techniques is disclosed. Also disclosed are methods for utilizing such mouse CC-CKR5 in the development of gene knockout mice for use as a model for human immunodeficiency virus.
摘要翻译： 公开了编码这种小鼠CC-CKR5的小鼠CC-CKR5多肽和DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了在基因敲除小鼠的开发中利用这种小鼠CC-CKR5用作人类免疫缺陷病毒模型的方法。
-
公开(公告)号：US5863798A
公开(公告)日：1999-01-26
申请号：US948222
申请日：1997-10-09
IPC分类号： G01N33/566 , C07K14/705 , C12N5/071 , C12N5/10 , C12N11/00 , C12N15/09 , C12N15/85 , C12P21/02 , C12R1/91 , G01N33/50 , C12N15/00
CPC分类号： G01N33/502 , C07K14/705 , C12N11/00 , C12N15/85 , C12N5/0686 , G01N33/5008 , C12N2501/50 , C12N2503/00 , C12N2510/00
摘要： Attachment enhanced human embryonic kidney cells, 293, are provided. These cells have been modified to contain a selected mammalian scavenger gene, which has been found to improve the ability of these cells to attach in culture. The improved cells of the invention are useful in assays in which the unmodified 293 cells could be used.
摘要翻译： 提供附着增强的人胚胎肾细胞293。 已经将这些细胞修饰成含有选择的哺乳动物清道夫基因，其被发现可以提高这些细胞附着在培养物中的能力。 本发明的改进的细胞可用于其中可以使用未修饰的293细胞的测定中。
-
5.发明授权Method of improving the yield of heterologous proteins produced by Streptomyces lividans 失效改善由菌体生成的异位蛋白质产生的方法
公开(公告)号：US5223418A
公开(公告)日：1993-06-29
申请号：US589979
申请日：1990-09-28
IPC分类号： C07K14/73 , C12N1/20 , C12N1/21 , C12N1/38 , C12N15/09 , C12N15/12 , C12N15/76 , C12P21/02 , C12R1/465
CPC分类号： C12N1/20 , C07K14/70514 , C12N15/76
摘要： A method is provided for improving the yield of the heterologous proteins sCD4 and derivatives of sCD4, produced by recombinant Streptomyces lividans by the addition of casamino acids to the nutrient medium in which the bacteria are cultured. Also provided by this invention is a method for improving the half-life of heterologous proteins in a cell free culture supernatant by the addition of casamino acids to the supernatant. Complex media are also provided.
摘要翻译： 提供了一种方法，用于通过向培养细菌的营养培养基中加入酪蛋白氨基酸来提高重组变色链霉菌（Streptomyces lividans）产生的异源蛋白质sCD4和sCD4衍生物的产量。 本发明还提供了通过向上清液中加入酪蛋白氨基酸来改善无细胞培养上清液中异源蛋白质半衰期的方法。 还提供复杂的媒体。
-
公开(公告)号：US5242809A
公开(公告)日：1993-09-07
申请号：US967949
申请日：1992-10-27
摘要： A recombinant DNA molecule comprising the Streptomyces gal operon galK gene; galE gene; galT gene; P1 promoter, P2 promoter, P2 promoter expression unit; P1 promoter regulated region; or the entire Streptomyces gal operon.
摘要翻译： 包含链霉菌gal操纵子galK基因的重组DNA分子; galE基因; galT基因; P1启动子，P2启动子，P2启动子表达单位; P1启动子调控区; 或整个Streptomyces gal操纵子。
-
公开(公告)号：US4717666A
公开(公告)日：1988-01-05
申请号：US681749
申请日：1984-12-14
IPC分类号： C12N1/20 , C12N9/38 , C12N15/56 , C12N15/67 , C12N15/72 , C12N15/76 , C12N15/00 , C07H15/12 , C12N1/00
CPC分类号： C12N15/67 , C12N15/72 , C12N15/76 , C12N9/2468 , C07K2319/02 , C07K2319/61 , Y10S435/886
摘要： A DNA fragment from Streptomyces sp. which contains a gene which can code for an excretable protein is isolated, inserted into a plamid vector and used to transform other Streptomycetes.
摘要翻译： 来自链霉菌属的DNA片段 其包含可以编码可排泄蛋白质的基因被分离，插入到氨基酰胺载体中并用于转化其他链霉菌。
-
-
-
-
-
-
搜索结果
7 条记录 (耗时 0.012 秒)
