公开(公告)号：US20150231635A1

公开(公告)日：2015-08-20

申请号：US14640471

申请日：2015-03-06

Applicant: On-Chip Cellomics Consortium

Inventor： Kazunori Okano ,  Kenji Yasuda

IPC: B01L7/00 ,  A01N1/02

CPC classification number: B01L7/50 ,  A01N1/0284 ,  G01N33/5061 ,  G01N33/566

Abstract: In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip.

公开(公告)号：US20150132766A1

公开(公告)日：2015-05-14

申请号：US14388154

申请日：2013-03-29

Applicant: KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY ,  ON-CHIP CELLOMICS CONSORTIUM ,  NATIONAL UNIVERSITY CORPORATION TOKYO MEDICAL AND DENTAL UNIVERSITY

Inventor： Kenji Yasuda ,  Hyonchol Kim ,  Hideyuki Terazono ,  Akihiro Hattori

IPC: G01N33/574 ,  G06T7/00 ,  G06K9/00

CPC classification number: G01N33/57496 ,  B01L3/502761 ,  C12M47/04 ,  G01N15/1434 ,  G01N15/1459 ,  G01N15/1463 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N21/6486 ,  G01N33/574 ,  G01N2015/1254 ,  G01N2015/149 ,  G01N2015/1497 ,  G06K9/00147 ,  G06T7/0012 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30024 ,  G06T2207/30101

Abstract: The present invention provides a cell enrichment/purification device having a function of continuously enriching cells, a function of locating the cells in a particular area of a flow path in a continuous array after the cell enrichment, a function of recognizing the shape of the cells and fluorescence emission from the cells at the same time in units of one cell based on an image, and a function of recognizing the cells based on the information on the shape and fluorescence emission to separate/purify the cells.

Abstract translation: 本发明提供了具有连续富集细胞的功能的细胞富集/纯化装置，在细胞富集后将细胞定位在连续阵列中的流路的特定区域中的功能，识别细胞形状的功能 以及基于图像以一个单元为单位的单元的荧光发射，以及基于关于形状和荧光发射的信息来识别细胞以分离/纯化细胞的功能。

公开(公告)号：US20150079598A1

公开(公告)日：2015-03-19

申请号：US14382638

申请日：2013-03-05

Applicant: KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY ,  ON-CHIP CELLOMICS CONSORTIUM

Inventor： Kenji Yasuda ,  Hideyuki Terazono ,  Hyonchol Kim ,  Akihiro Hattori

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/12 ,  B01L2200/142 ,  B01L2200/147 ,  B01L2300/0654 ,  B01L2300/0851 ,  B01L2300/1822 ,  B01L2300/1827 ,  B01L2300/185 ,  B01L2400/0622 ,  B01L2400/0638 ,  B01L2400/065 ,  C12Q2565/629

Abstract: The present invention provides a high-speed gene amplification device including an additional mechanism that allows more stable temperature control, a pre-treatment mechanism that performs pre-treatment including a pre-PCR reaction reverse transcription reaction process that allows RNA detection, a melting curve analysis function, chip technology optimal for holding liquid droplets and performing optical measurements and an optical measurement function for a PCR reaction.

Abstract translation: 本发明提供了一种包含能够进行更稳定的温度控制的附加机理的高速基因扩增装置，进行前处理的预处理机理，包括允许RNA检测的PCR前反应逆转录反应过程，熔解曲线 分析功能，用于保持液滴和进行光学测量的最佳芯片技术和用于PCR反应的光学测量功能。

公开(公告)号：US20140370492A1

公开(公告)日：2014-12-18

申请号：US14360741

申请日：2012-11-27

Applicant: Kanagawa Academy of Science and Technology ,  On-Chip Cellomics Consortium ,  National University Corporation Tokyo Medical and Dental University

Inventor： Kenji Yasuda ,  Hideyuki Terazono ,  Akihiro Hattori

IPC: C12M1/02 ,  C12Q1/68

CPC classification number: C12M41/18 ,  B01L3/50851 ,  B01L3/50855 ,  B01L7/02 ,  B01L7/52 ,  B01L2200/025 ,  B01L2200/147 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/1838 ,  B01L2300/185 ,  B01L2400/0478 ,  B01L2400/0644 ,  B01L2400/065 ,  C12Q1/6806 ,  C12Q1/6846 ,  G01N21/6452 ,  C12Q2565/629

Abstract: The present invention provides a liquid reflux reaction control device including an additional mechanism that allows more stable temperature control, a pre-treatment mechanism that performs pre-treatment including a pre-PCR reaction reverse transcription reaction process that allows RNA detection, a melting curve analysis function, chip technology optimal for holding liquid droplets and optical measurement and the optical measurement function for PCR, and a temperature gradient control mechanism using a quantitative infrared light irradiation/absorption control technique.

Abstract translation: 本发明提供一种液体回流反应控制装置，其包括允许更稳定的温度控制的附加机理，执行预处理的预处理机制，包括允许RNA检测的PCR前反应逆转录反应过程，熔解曲线分析 功能，保持液滴和光学测量最佳的芯片技术和PCR的光学测量功能，以及使用定量红外光照射/吸收控制技术的温度梯度控制机制。