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    Process for labeling nucleic acids and hybridization probes
    1.
    发明授权
    Process for labeling nucleic acids and hybridization probes 失效
    标记核酸和杂交探针的方法

    公开(公告)号：US4617261A

    公开(公告)日：1986-10-14

    申请号：US791332

    申请日：1985-10-25

    Applicant: Edward L. Sheldon, III ,  Corey H. Levenson ,  Kary B. Mullis ,  Henry Rapoport ,  Robert M. Watson

    Inventor： Edward L. Sheldon, III ,  Corey H. Levenson ,  Kary B. Mullis ,  Henry Rapoport ,  Robert M. Watson

    IPC: C07D493/04 ,  C07D513/04 ,  C07D519/00 ,  C07H21/00 ,  C12Q1/68 ,  G01N33/48

    CPC classification number: C07D493/04 ,  C07D513/04 ,  C07H21/00 ,  C12Q1/68 ,  Y10T436/143333

    Abstract: Nucleic acids may be labeled by intercalating the alkylating intercalation moiety of a labeling reagent into a partially double-stranded nucleic acid to form a complex and activating the complex to cause covalent bonding between the reagent and the nucleic acid. Preferably, the labeled nucleic acid is a hybridization probe for detecting nucleic acid sequences capable of hybridizing with a hybridizing region of the nucleic acid. Also preferably the label moiety is non-radioactive. The labeling reagent is of the formula:[A--[B--Lwhere A is an alkylating intercalation moiety, B is a divalent organic moiety of the formula: ##STR1## where Y is O, NH or N--CHO, x is a number from 1 to 4, y is a number from 2 to 4, and L is a monovalent label moiety, wherein B is exclusive of any portion of the intercalation and label moieties.Preferably A is a 4-methylene-substituted psoralen moiety, and most preferably A is a 4'-methylene-substituted-4,5',8-trimethylpsoralen moeity and L is biotin.

    Abstract translation: 可以通过将标记试剂的烷基化插层部分嵌入部分双链核酸中以形成复合物并激活复合物以引起试剂和核酸之间的共价键来标记核酸。 优选地，标记的核酸是用于检测能够与核酸的杂交区域杂交的核酸序列的杂交探针。 还优选地，标记部分是非放射性的。 标记试剂具有下式：[A- [BL，其中A是烷基化插层部分，B是下式的二价有机部分：其中Y是O，NH或N-CHO，x是从 1至4，y为2至4的数，L为单价标记部分，其中B不包括插层和标记部分的任何部分。 优选A是4-亚甲基取代的补骨脂素部分，最优选A是4'-亚甲基取代的-4,5'，8-三甲基肉桂酸酯，L是生物素。

    Method of and apparatus for extending signal ranges of digital images
    8.
    发明授权
    Method of and apparatus for extending signal ranges of digital images 有权
    扩展数字图像信号范围的方法和装置

    公开(公告)号：US06909459B2

    公开(公告)日：2005-06-21

    申请号：US10465013

    申请日：2003-06-18

    Applicant: Robert M. Watson, Jr. ,  John J. Kang

    Inventor： Robert M. Watson, Jr. ,  John J. Kang

    IPC: G06T5/50 ,  H04N5/353 ,  H04N5/355 ,  H04N5/361 ,  H04N5/365 ,  H04N5/235

    CPC classification number: G06T5/50 ,  H04N5/2355

    Abstract: The invention is a method and apparatus to extend the signal range of a digital image beyond the nominal sensor or data format range. The method and apparatus automatically acquires a scaled series of source data, applies noise reduction to the source data, and constructs a scaled composite with usable signal ranges greater than that of the individual data sources. Applied to digital images, the invention permits presentation and analysis of all signals from a subject in a single composite or an image resulting from the method and apparatus of the present invention. The present invention overcomes two defects in prior art systems: increased noise in the resultant composite image arising from rescaling of component images and dependence on evaluating image content to determine image scaling. Because this invention can be automated, it can be applied in numerous fields requiring high throughput.

    Abstract translation: 本发明是一种将数字图像的信号范围扩展超过标称传感器或数据格式范围的方法和装置。 该方法和装置自动获取缩放的一系列源数据，对源数据应用降噪，并构建具有大于各个数据源的可用信号范围的缩放复合。 本发明适用于数字图像，允许从由本发明的方法和装置产生的单个复合物或图像中的受试者的所有信号的呈现和分析。 本发明克服了现有技术系统中的两个缺点：增加了由组件图像的重新缩放产生的合成图像中的噪声，并且依赖于评估图像内容以确定图像缩放。 因为本发明可以自动化，所以可以应用于需要高通量的许多领域。

    Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
    10.
    发明授权
    Methods for in-solution quenching of fluorescently labeled oligonucleotide probes 失效
    溶液淬灭荧光标记寡核苷酸探针的方法

    公开(公告)号：US5491063A

    公开(公告)日：1996-02-13

    申请号：US299682

    申请日：1994-09-01

    Applicant: Mary E. Fisher ,  Robert M. Watson

    Inventor： Mary E. Fisher ,  Robert M. Watson

    IPC: G01N21/64 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/566 ,  C12Q1/70 ,  C07H19/04 ,  C12P19/34

    CPC classification number: C12Q1/6818 ,  C12Q1/6823

    Abstract: The present invention is directed to methods for controlling the light emission of an oligonucleotide labeled with a light-emitting label that are useful in nucleic acid detection assays. A reaction that results in the cleavage of single-stranded oligonucleotide probes labeled with a light-emitting label is carried out in the presence of a DNA binding compound that interacts with the label to modify the light emission of the label. The methods utilize the change in light emission of the labeled probe that results from degradation of the probe. The methods are applicable in general to assays that utilize a reaction that results in cleavage of oligonucleotide probes, and, in particular, to homogeneous amplification/detection assays wherein hybridized probe is cleaved concomitant with palmer extension. A homogeneous amplification/detection assay is provided which allows the simultaneous detection of the accumulation of amplified target and the sequence-specific detection of the target sequence.

    Abstract translation: 本发明涉及用于控制用于核酸检测测定中的用发光标记标记的寡核苷酸的发光的方法。 导致用发光标记物标记的单链寡核苷酸探针的切割的反应在与标记物相互作用以修饰标记的光发射的DNA结合化合物的存在下进行。 该方法利用由探针降解引起的标记探针的发光变化。 所述方法一般适用于利用导致寡核苷酸探针切割的反应的测定，特别是适用于其中杂交探针与帕默​​延伸一起裂解的均质扩增/检测测定。 提供了均一的扩增/检测测定法，其允许同时检测扩增的靶标的积累和靶序列的序列特异性检测。

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