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公开(公告)号：US07731827B2
公开(公告)日：2010-06-08
申请号：US11155150
申请日：2005-06-17
申请人： Juan Astorga-Wells , Sang-Ryoul Park , Harold Swerdlow , Gerald Jesson , Ulf Lindberg , Tomas Bergman , Hans Jornvall , Mats Jonsson
发明人： Juan Astorga-Wells , Sang-Ryoul Park , Harold Swerdlow , Gerald Jesson , Ulf Lindberg , Tomas Bergman , Hans Jornvall , Mats Jonsson
IPC分类号： B01D61/44
CPC分类号： G01N1/405 , B01L3/502753 , B01L2300/0681 , B01L2300/0864 , B01L2400/0421 , B01L2400/0487 , G01N27/44769 , G01N2001/4011
摘要： The invention relates to a method for capturing charged molecules of interest traveling in an electrolyte flow stream through an electrically non-conductive channel, comprising at least one anode and at least one cathode individually separated from said channel, but in electrical contact with said flow stream, by a conductive ion selective semi-permeable membrane. Said membrane interferes with the normal migration of ions towards its respective electrode, generating at least two zones of different electric field. Balance between hydrodynamic and electrical forces captures certain ions into the flow stream. it also relates to a device for performing the method.
摘要翻译： 本发明涉及一种用于捕获通过非导电通道在电解液流中行进的感兴趣的带电分子的方法，该方法包括至少一个阳极和至少一个与所述通道分离的阳极，但是与所述流动流 ，通过导电离子选择性半透膜。 所述膜干扰离子向其相应电极的正常迁移，产生至少两个不同电场的区域。 流体动力学和电力之间的平衡将某些离子捕获到流动流中。 它还涉及一种用于执行该方法的装置。
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公开(公告)号：US07294467B2
公开(公告)日：2007-11-13
申请号：US11138981
申请日：2005-05-26
申请人： Sang-Ryoul Park , In-Chul Yang , Myung-Sub Han , Yong-Hyeon Yim , Eui-Jin Hwang
发明人： Sang-Ryoul Park , In-Chul Yang , Myung-Sub Han , Yong-Hyeon Yim , Eui-Jin Hwang
CPC分类号： C12Q1/68 , C12Q2545/114 , C12Q2523/301 , C12Q2523/107
摘要： The present invention is related to a novel approach for quantitation of DNA based on the stoichiometric existence of phosphorus (P) in DNA.
摘要翻译： 本发明涉及基于DNA中磷（P）的化学计量存在的DNA定量的新方法。
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3.发明授权Separation method for multi channel electrophoresis device having no individual sample wells 有权没有单个样品孔的多通道电泳装置的分离方法公开(公告)号：US07993507B2
公开(公告)日：2011-08-09
申请号：US11791509
申请日：2005-11-16
申请人： Sang-Ryoul Park , In-Chul Yang , Young-Ho Kim
发明人： Sang-Ryoul Park , In-Chul Yang , Young-Ho Kim
IPC分类号： G01N27/26 , G01N27/447
CPC分类号： G01N27/44782 , B01L9/527 , G01N27/44791
摘要： The present invention is related to a method of separation of compounds by electrophoresis in which the compounds such as genes, proteins, etc. may be analyzed very precisely as samples are introduced directly into the separation tubes of the chip at the collection site, and therefore, it is not necessary to have separate fluid paths or individual sample storing apparatus that have been necessary for the conventional electrophoresis; it is easy to make the chips as the structure of the chip becomes extremely simple, and high-density arrangement of the separation tubes is enabled; and further, the compounds such as genes, proteins, etc. may be analyzed very precisely without interference in the storage tubs by using a non-polar solvent as the solvent of the sample storage tub.
摘要翻译： 本发明涉及通过电泳分离化合物的方法，其中可以非常精确地分析诸如基因，蛋白质等化合物，因为样品在采集部位直接引入芯片的分离管中，因此 ，不需要为常规电泳所必需的单独的流体路径或单独的样品存储装置; 由于芯片的结构变得非常简单，因此容易制造芯片，并且能够实现高密度的分离管布置; 此外，通过使用非极性溶剂作为样品储存桶的溶剂，可以非常精确地分析诸如基因，蛋白质等化合物而不会干扰存储桶。
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4.发明申请Separation Method For Multi Channel Electrophoresis Device Having No Individual Sample Wells 有权没有单个样品孔的多通道电泳装置的分离方法公开(公告)号：US20080047834A1
公开(公告)日：2008-02-28
申请号：US11791509
申请日：2005-11-16
申请人： Sang-Ryoul Park , In-Chul Yang , Young-Ho Kim
发明人： Sang-Ryoul Park , In-Chul Yang , Young-Ho Kim
IPC分类号： B01D57/02
CPC分类号： G01N27/44782 , B01L9/527 , G01N27/44791
摘要： The present invention is related to a method of separation of compounds by electrophoresis in which the compounds such as genes, proteins, etc. may be analyzed very precisely as samples are introduced directly into the separation tubes of the chip at the collection site, and therefore, it is not necessary to have separate fluid paths or individual sample storing apparatus that have been necessary for the conventional electrophoresis; it is easy to make the chips as the structure of the chip becomes extremely simple, and high-density arrangement of the separation tubes is enabled; and further, the compounds such as genes, proteins, etc. may be analyzed very precisely without interference in the storage tubs by using a non-polar solvent as the solvent of the sample storage tub.
摘要翻译： 本发明涉及通过电泳分离化合物的方法，其中可以非常精确地分析诸如基因，蛋白质等化合物，因为样品在采集部位直接引入芯片的分离管中，因此 ，不需要为常规电泳所必需的单独的流体路径或单独的样品存储装置; 由于芯片的结构变得非常简单，因此容易制造芯片，并且能够实现高密度的分离管布置; 此外，通过使用非极性溶剂作为样品储存桶的溶剂，可以非常精确地分析诸如基因，蛋白质等化合物而不会干扰存储桶。
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公开(公告)号：US09297038B2
公开(公告)日：2016-03-29
申请号：US13989729
申请日：2011-11-09
申请人： In Chul Yang , Min Jung Kang , Han Nah Yu , Sang Ryoul Park , Sook Kyoung Kim
发明人： In Chul Yang , Min Jung Kang , Han Nah Yu , Sang Ryoul Park , Sook Kyoung Kim
CPC分类号： C12Q1/686 , C12Q2525/179 , C12Q2561/113
摘要： Disclosed are a method and a kit for the quantification of nucleic acids, especially a trace amount of nucleic acid, such as host cell nucleic acid impurities, using real-time PCR with a random primer.
摘要翻译： 公开了使用具有随机引物的实时PCR来定量核酸，特别是痕量的核酸，例如宿主细胞核酸杂质的方法和试剂盒。
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公开(公告)号：US20130295577A1
公开(公告)日：2013-11-07
申请号：US13989729
申请日：2011-11-09
申请人： In Chul Yang , Min Jung Kang , Han Nah Yu , Sang Ryoul Park , Sook Kyoung Kim
发明人： In Chul Yang , Min Jung Kang , Han Nah Yu , Sang Ryoul Park , Sook Kyoung Kim
IPC分类号： C12Q1/68
CPC分类号： C12Q1/686 , C12Q2525/179 , C12Q2561/113
摘要： Disclosed are a method and a kit for the quantification of nucleic acids, especially a trace amount of nucleic acid, such as host cell nucleic acid impurities, using real-time PCR with a random primer.
摘要翻译： 公开了使用具有随机引物的实时PCR来定量核酸，特别是痕量的核酸，例如宿主细胞核酸杂质的方法和试剂盒。
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公开(公告)号：US20060078905A1
公开(公告)日：2006-04-13
申请号：US11138981
申请日：2005-05-26
申请人： Sang-Ryoul Park , In-Chul Yang , Myung-Sub Han , Yong-Hyeon Yim , Eui-Jin Hwang
发明人： Sang-Ryoul Park , In-Chul Yang , Myung-Sub Han , Yong-Hyeon Yim , Eui-Jin Hwang
IPC分类号： C12Q1/68
CPC分类号： C12Q1/68 , C12Q2545/114 , C12Q2523/301 , C12Q2523/107
摘要： The present invention is related to a novel approach for quantitation of DNA based on the stoichiometric existence of phosphorus (P) in DNA.
摘要翻译： 本发明涉及基于DNA中磷（P）的化学计量存在的DNA定量的新方法。
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8.发明申请Method and device for capturing charged molecules traveling in a flow stream 有权用于捕获在流动流中行进的带电分子的方法和装置公开(公告)号：US20050284762A1
公开(公告)日：2005-12-29
申请号：US11155150
申请日：2005-06-17
申请人： Juan Astorga-Wells , Sang-Ryoul Park , Harold Swerdlow , Gerald Jesson , Ulf Lindberg , Tomas Bergman , Hans Jornvall , Mats Jonsson
发明人： Juan Astorga-Wells , Sang-Ryoul Park , Harold Swerdlow , Gerald Jesson , Ulf Lindberg , Tomas Bergman , Hans Jornvall , Mats Jonsson
IPC分类号： B01D57/02 , B01L3/00 , B81B3/00 , G01N20060101 , G01N1/34 , G01N27/404 , G01N27/447
CPC分类号： G01N1/405 , B01L3/502753 , B01L2300/0681 , B01L2300/0864 , B01L2400/0421 , B01L2400/0487 , G01N27/44769 , G01N2001/4011
摘要： The invention relates to a method for capturing charged molecules of interest traveling in an electrolyte flow stream through an electrically non-conductive channel, comprising at least one anode and at least one cathode individually separated from said channel, but in electrical contact with said flow stream, by a conductive ion selective semi-permeable membrane. Said membrane interferes with the normal migration of ions towards its respective electrode, generating at least two zones of different electric field. Balance between hydrodynamic and electrical forces captures certain ions into the flow stream. it also relates to a device for performing the method.
摘要翻译： 本发明涉及一种用于捕获通过非导电通道在电解液流中行进的感兴趣的带电分子的方法，该方法包括至少一个阳极和至少一个与所述通道分离的阳极，但是与所述流动流 ，通过导电离子选择性半透膜。 所述膜干扰离子向其相应电极的正常迁移，产生至少两个不同电场的区域。 流体动力学和电力之间的平衡将某些离子捕获到流动流中。 它还涉及一种用于执行该方法的装置。
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