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20 条记录 (耗时 0.019 秒)

    DNA sequencing
    1.
    发明授权
    DNA sequencing 失效
    DNA测序

    公开(公告)号：US5409811A

    公开(公告)日：1995-04-25

    申请号：US869520

    申请日：1992-04-16

    申请人： Stanley Tabor ,  Charles C. Richardson

    发明人： Stanley Tabor ,  Charles C. Richardson

    IPC分类号： C12Q1/42 ,  C12Q1/48 ,  C12Q1/68 ,  C12P19/34

    CPC分类号： C12Q1/6869 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S436/808 ,  Y10T436/143333

    摘要： A method for sequencing a strand of DNA, including the steps off: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with four deoxyribonucleoside triphosphates, a DNA polymerase, and at least three deoxyribonucleoside triphosphates in different amounts, under conditions in favoring primer extension to form nucleic acid fragments complementory to the DNA to be sequenced; labelling the nucleic and fragments; separating them and determining the position of the deoxyribonucleoside triphosphates by differences in the intensity of the labels, thereby to determine the DNA sequence.

    摘要翻译： 一种测序DNA链的方法，包括步骤：提供DNA链; 用能够与链杂交的引物退火链，得到退火混合物; 在有利于引物延伸以形成与要测序的DNA互补的核酸片段的条件下，将四种脱氧核糖核苷三磷酸，DNA聚合酶和至少三种脱氧核糖核苷三磷酸一起孵育， 标记核酸和片段; 分离它们并通过标记强度的差异确定脱氧核糖核苷三磷酸的位置，从而确定DNA序列。

    DNA sequencing
    3.
    发明授权
    DNA sequencing 失效
    DNA测序

    公开(公告)号：US4962020A

    公开(公告)日：1990-10-09

    申请号：US218103

    申请日：1988-07-12

    申请人： Stanley Tabor ,  Charles C. Richardson

    发明人： Stanley Tabor ,  Charles C. Richardson

    IPC分类号： C12Q1/42 ,  C12Q1/48 ,  C12Q1/68

    CPC分类号： C12Q1/6869 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S436/808 ,  Y10T436/143333

    摘要： A method for sequencing a strand of DNA, including the steps of: providing the strand of DNA; annealing the strand with a primer able to hybridize to the strand to give an annealed mixture; incubating the mixture with a deoxyribonucleoside triphosphate, a DNA polymerase, and a chain terminating agent under conditions in which the polymerase causes the primer to be elongated to form a series of DNA products differing in length of the elongated primer, each DNA product having a chain terminating agent at its elongated end; the number of each DNA product being approximately the same for substantially all DNA products differing in length from 1 to 20 bases.

    Isothermal amplification of DNA
    5.
    发明申请
    Isothermal amplification of DNA 有权
    DNA的等温扩增

    公开(公告)号：US20050164213A1

    公开(公告)日：2005-07-28

    申请号：US10813693

    申请日：2003-11-07

    申请人： Stanley Tabor ,  Charles Richardson

    发明人： Stanley Tabor ,  Charles Richardson

    IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/6844 ,  C12Q2527/101 ,  C12Q2523/313 ,  C12Q2522/101 ,  C12Q2521/525 ,  C12Q2521/513 ,  C12Q2521/501

    摘要： A method of amplifying a template DNA molecule comprising incubating the template DNA molecule in a reaction mixture comprising a DNA polymerase and at least one accessory protein at a constant temperature to produce amplified product, wherein production of amplified product does not require exogenously-added oligonucleotide primers and the template DNA molecule does not have have terminal protein covalently bound to either 5′ end.

    摘要翻译： 包括扩增模板DNA分子的方法，包括在恒温下将包含DNA聚合酶和至少一种辅助蛋白质的反应混合物中的模板DNA分子孵育以产生扩增产物，其中扩增产物的产生不需要外源添加的寡核苷酸引物 并且模板DNA分子不具有与5'末端共价结合的末端蛋白质。

    DNA sequencing with pyrophosphatase
    6.
    发明授权
    DNA sequencing with pyrophosphatase 失效
    利用焦磷酸酶进行DNA测序

    公开(公告)号：US5498523A

    公开(公告)日：1996-03-12

    申请号：US275339

    申请日：1994-07-14

    申请人： Stanley Tabor ,  Charles C. Richardson

    发明人： Stanley Tabor ,  Charles C. Richardson

    IPC分类号： C12N9/16 ,  C12Q1/68 ,  C12N9/14 ,  C12P19/34 ,  G01N33/50

    CPC分类号： C12N9/16 ,  C12Q1/686 ,  Y10T436/143333

    摘要： A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

    摘要翻译： 一种用于延伸寡核苷酸引物的试剂盒或溶液，所述寡核苷酸引物在具有与第一单链区同源的第二单链区的模板分子上具有第一单链区，其包含能够引起第一 在反应混合物中在模板的第二单链区上的引物的单链区域，以及能够将反应混合物中的焦磷酸盐的量降低到在不存在第二次的情况下在扩展期间产生的量的第二试剂 代理商

    Method for nucleic acid hybridization using single-stranded DNA binding protein
    9.
    发明授权
    Method for nucleic acid hybridization using single-stranded DNA binding protein 失效
    使用单链DNA结合蛋白进行核酸杂交的方法

    公开(公告)号：US5534407A

    公开(公告)日：1996-07-09

    申请号：US229396

    申请日：1994-04-18

    申请人： Stanley Tabor ,  Charles C. Richardson

    发明人： Stanley Tabor ,  Charles C. Richardson

    IPC分类号： C12Q1/68 ,  C12Q1/70

    CPC分类号： C12Q1/6832 ,  C12Q1/6813

    摘要： Method of nucleic acid hybridization for detecting the presence of a specific nucleic acid sequence in a population of different nucleic acid sequences using a nucleic acid probe. The nucleic acid probe hybridizes with the specific nucleic acid sequence but not with other nucleic acid sequences in the population. The method includes contacting a sample (potentially including the nucleic acid sequence) with the nucleic acid probe under hybridizing conditions in the presence of a single-stranded DNA binding protein provided in an amount which stimulates renaturation of a dilute solution (i.e., one in which the t.sub.1/2 of renaturation is longer than 3 weeks) of single-stranded DNA greater than 500 fold (i.e., to a t.sub.1/2 less than 60 min, preferably less than 5 min, and most preferably about 1 min.) in the absence of nucleotide triphosphates.

    摘要翻译： 使用核酸探针检测不同核酸序列群体中特定核酸序列的存在的核酸杂交方法。 核酸探针与特定核酸序列杂交，但不与群体中的其他核酸序列杂交。 该方法包括在杂交条件下，在单链DNA结合蛋白存在下将样品（可能包括核酸序列）与核酸探针接触，所述单链DNA结合蛋白以刺激稀溶液复性的量提供（即，其中 大于500倍（即，t1 / 2小于60分钟，优选小于5分钟，最优选约1分钟）的单链DNA的复性的t1 / 2长于3周） 缺乏三磷酸核苷酸。