公开(公告)号：US20130157254A1

公开(公告)日：2013-06-20

申请号：US13374225

申请日：2011-12-16

Applicant: Atanu SENGUPTA ,  Frank E. INSCORE ,  Chetan SHENDE ,  Michael J. DONAHUE ,  Stuart FARQUHARSON

Inventor： Atanu SENGUPTA ,  Frank E. INSCORE ,  Chetan SHENDE ,  Michael J. DONAHUE ,  Stuart FARQUHARSON

IPC: G01N21/65 ,  C12M1/40 ,  C12M1/34 ,  C12Q1/70

CPC classification number: G01N21/658 ,  G01N33/54373 ,  G01N33/58 ,  G01N33/587

Abstract: A SERS method and apparatus employ a sample device having support structure including a first material containing a SER-active metal functionalized with a binding agent having specific capability for binding a designated target analyte. An analyte sample is introduced upon the functionalized SER-active metal; conditions to effect binding of the target analyte to the binding agent are maintained; unbound chemicals, biochemicals, or biologicals are removed; a second SER-active material is introduced to cause it to attach to the bound target analyte; the support structure is irradiated to generate a SER spectrum, with the first and second SER-active materials acting in concert; and the SER spectrum is detected and analyzed to determine the presence and quantity of the target analyte. Alternatively, the second SER-active material may be functionalized with a binding agent, with the procedure being modified accordingly.

公开(公告)号：US20100211329A1

公开(公告)日：2010-08-19

申请号：US12734091

申请日：2008-10-10

Applicant: Stuart Farquharson ,  Wayne W. Smith

Inventor： Stuart Farquharson ,  Wayne W. Smith

IPC: G06F19/00

CPC classification number: G01N21/359 ,  G01N21/3577 ,  G01N21/65 ,  G01N21/84 ,  G01N2021/6421 ,  G01N2021/8411 ,  G01N2201/1293

Abstract: The method and apparatus are used to determine class, grade and properties of fuel samples, regardless of ambient, instrument, or sample temperature, using mathematical correlations between fuel class, grade and properties and their spectra developed from a database of samples with measured properties and spectra. The ability to measure a fuel sample using the present method and apparatus is useful in identifying unknown fuel samples, determining suitability in equipment, and monitoring and controlling fuel processes, such as blending operations, distillation, and synthesis.

Abstract translation: 该方法和设备用于确定燃料样品的等级，等级和性能，无论环境，仪器或样品温度如何，使用燃料类别，等级和性质之间的数学相关性以及从具有测量性质的样品数据库开发的光谱，以及 光谱。 使用本方法和装置测量燃料样品的能力可用于识别未知燃料样品，确定设备的适用性，以及监测和控制燃料过程，例如混合操作，蒸馏和合成。

公开(公告)号：US07393692B2

公开(公告)日：2008-07-01

申请号：US11098844

申请日：2005-04-05

Applicant: Stuart Farquharson ,  Frank E. Inscore ,  Alan D. Gift ,  Chetan S. Shende

Inventor： Stuart Farquharson ,  Frank E. Inscore ,  Alan D. Gift ,  Chetan S. Shende

IPC: G01N21/65 ,  G01N30/14 ,  G01N33/15 ,  G01N30/88

CPC classification number: G01N21/658 ,  Y10T436/141111 ,  Y10T436/147777 ,  Y10T436/163333 ,  Y10T436/25375 ,  Y10T436/255

Abstract: The method and apparatus rapidly separate drugs and their metabolites from saliva and, in a continuous sequence of steps, rapidly detect, identify and quantify them through surface-enhanced Raman spectroscopy.

Abstract translation: 该方法和装置将药物及其代谢物从唾液中快速分离，并以连续的步骤顺序通过表面增强拉曼光谱快速检测，鉴定和定量。

公开(公告)号：US20060257891A1

公开(公告)日：2006-11-16

申请号：US11355782

申请日：2006-02-16

Applicant: Stuart Farquharson ,  Frank Inscore ,  Alan Gift ,  Chetan Shende

Inventor： Stuart Farquharson ,  Frank Inscore ,  Alan Gift ,  Chetan Shende

IPC: C12Q1/68

CPC classification number: C12Q1/04 ,  G01N2333/32 ,  G01N2333/33 ,  Y02A50/451

Abstract: A weak organic acid is used to effect the release of CaDPA from Bacillus or Clostridium endospores, rapidly and at room temperature, to enable detection and measurement of DPA and thereby the assessment of risk associated with exposure to Bacillus anthracis, Clostridium botulinum, and like spores. The method can be applied to airborne, food-borne, and water-borne spores, as well as to spores collected from surfaces or contained in body fluids, and analysis is advantageously carried out using surface-enhanced Raman spectroscopy.

Abstract translation: 使用弱有机酸快速和室温下从芽孢杆菌或梭状芽孢杆菌内孢子中释放CaDPA，以便能够检测和测量DPA，从而评估与接触炭疽芽孢杆菌，肉毒梭菌和类似孢子相关的风险 。 该方法可以应用于空气传播，食源性和水性孢子，以及从表面收集的或包含在体液中的孢子，并且有利地使用表面增强拉曼光谱进行分析。

公开(公告)号：US20050282287A1

公开(公告)日：2005-12-22

申请号：US11193894

申请日：2005-07-29

Applicant: Stuart Farquharson ,  Paul Maksymiuk

Inventor： Stuart Farquharson ,  Paul Maksymiuk

IPC: B01L3/00 ,  B01L3/02 ,  G01N1/40 ,  G01N21/65 ,  G01N30/60 ,  G01N30/74 ,  G01N30/78 ,  B01L11/00

CPC classification number: G01N21/658 ,  B01L3/0275 ,  B01L3/5023 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/087 ,  B01L2400/0457 ,  G01N1/40 ,  G01N30/6082 ,  G01N30/74 ,  G01N30/78 ,  G01N21/63

Abstract: Sol-gel beds and deposits are utilized for SERS analysis of liquid analytes. Measurements are made at multiple points along the length of a column or channel to increase, very significantly, the speed of analysis, and use of the same medium to both separate the chemicals and also for SERS greatly reduces the complexity of such apparatus and enhances the efficiency of the method.

Abstract translation: 溶胶凝胶床和沉积物用于液体分析物的SERS分析。 在柱或通道的长度上的多个点进行测量，以非常显着地增加分析速度，并且使用相同的介质来分离化学品并且还用于SERS，大大降低了这种装置的复杂性并且增强了 方法的效率。

公开(公告)号：US09134247B2

公开(公告)日：2015-09-15

申请号：US13374225

申请日：2011-12-16

Applicant: Atanu Sengupta ,  Frank E. Inscore ,  Chetan Shende ,  Michael J. Donahue ,  Stuart Farquharson

Inventor： Atanu Sengupta ,  Frank E. Inscore ,  Chetan Shende ,  Michael J. Donahue ,  Stuart Farquharson

IPC: G01N21/65 ,  G01N33/58

CPC classification number: G01N21/658 ,  G01N33/54373 ,  G01N33/58 ,  G01N33/587

Abstract: A SERS method and apparatus employ a sample device having support structure including a first material containing a SER-active metal functionalized with a binding agent having specific capability for binding a designated target analyte. An analyte sample is introduced upon the functionalized SER-active metal; conditions to effect binding of the target analyte to the binding agent are maintained; unbound chemicals, biochemicals, or biologicals are removed; a second SER-active material is introduced to cause it to attach to the bound target analyte; the support structure is irradiated to generate a SER spectrum, with the first and second SER-active materials acting in concert; and the SER spectrum is detected and analyzed to determine the presence and quantity of the target analyte. Alternatively, the second SER-active material may be functionalized with a binding agent, with the procedure being modified accordingly.

Abstract translation: SERS方法和装置使用具有支撑结构的样品装置，该样品装置包括含有用具有特异性结合指定靶标分析物能力的结合剂官能化的SER-活性金属的第一材料。 将分析物样品引入功能化的SER-活性金属上; 维持目标分析物与结合剂结合的条件; 未结合的化学物质，生物化学物质或生物制品被去除; 引入第二SER活性材料以使其附着于结合的靶分析物; 照射支撑结构以产生SER光谱，其中第一和第二SER活性材料一致; 并检测和分析SER光谱，以确定目标分析物的存在和数量。 或者，第二SER活性材料可以用粘合剂进行官能化，其过程被相应地改变。

公开(公告)号：US07462492B2

公开(公告)日：2008-12-09

申请号：US11193894

申请日：2005-07-29

Applicant: Stuart Farquharson ,  Paul Maksymiuk

Inventor： Stuart Farquharson ,  Paul Maksymiuk

IPC: G01N21/76

CPC classification number: G01N21/658 ,  B01L3/0275 ,  B01L3/5023 ,  B01L2300/0816 ,  B01L2300/0838 ,  B01L2300/087 ,  B01L2400/0457 ,  G01N1/40 ,  G01N30/6082 ,  G01N30/74 ,  G01N30/78 ,  G01N21/63

Abstract: Sol-gel beds and deposits are utilized for SERS analysis of liquid analytes. Measurements are made at multiple points along the length of a column or channel to increase, very significantly, the speed of analysis, and use of the same medium to both separate the chemicals and also for SERS greatly reduces the complexity of such apparatus and enhances the efficiency of the method.

Abstract translation: 溶胶凝胶床和沉积物用于液体分析物的SERS分析。 在柱或通道的长度上的多个点进行测量，以非常显着地增加分析速度，并且使用相同的介质来分离化学品并且还用于SERS，大大降低了这种装置的复杂性并且增强了 方法的效率。

公开(公告)号：US07312088B2

公开(公告)日：2007-12-25

申请号：US10902511

申请日：2004-07-29

Applicant: Stuart Farquharson

Inventor： Stuart Farquharson

IPC: G01N21/77

CPC classification number: G01N21/658 ,  G01N21/278

Abstract: Apparatus for use in effecting surface-enhanced Raman spectroscopy comprises first and second containment means, the first containment means containing a known quantity of a reference chemical having an effective surface-enhanced Raman factor, and the second containment means containing a surface-enhanced Raman-active medium and being sufficiently transparent, at least at one optical access location, to permit both the excitation irradiation of, and also the collection of surface-enhanced Raman scattered radiation from, a common field of view of the surface-enhanced Raman-active medium. The apparatus is constructed for carrying out the method of the invention; i.e., for effecting intimate mixing, substantially prior to introduction to the surface-enhanced Raman-active medium contained in the second containment means, of the reference chemical with an analyte chemical-containing solution introduced through an entrance into the first containment means. The homogeneous test solution so formed permeates the SER-active material in the second containment means, which is irradiated at the common field of view so as to produce SER scattered radiation for collection and quantitative analysis.

Abstract translation: 用于实现表面增强拉曼光谱的装置包括第一和第二容纳装置，第一容纳装置含有已知量的具有有效表面增强拉曼因子的参考化学品，第二容纳装置含有表面增强拉曼光谱， 至少在一个光学接近位置处，至少在一个光学接近位置处具有足够的透明度，以允许来自表面增强拉曼活性介质的共同视野的激发照射和表面增强拉曼散射辐射的收集 。 该装置被构造用于实施本发明的方法; 即在进入第二容纳装置中的表面增强拉曼活性介质引入参考化学品之前，通过入口进入第一容纳装置，进行紧密混合。 如此形成的均匀试验溶液渗透到第二容纳装置中的SER活性物质，其在公共视野下照射，以产生用于收集和定量分析的SER散射辐射。

公开(公告)号：US06623977B1

公开(公告)日：2003-09-23

申请号：US09704818

申请日：2000-11-02

Applicant: Stuart Farquharson ,  Chad Nelson ,  Yuan-Hsiang Lee

Inventor： Stuart Farquharson ,  Chad Nelson ,  Yuan-Hsiang Lee

IPC: G01N2165

CPC classification number: G01N21/658 ,  B82Y15/00 ,  G01N21/77

Abstract: Metal-doped sol-gel materials, suitable for use as sensors for surface-enhanced Raman spectroscopic analysis for trace chemical detection, are produced by effecting gelation and solvent removal of a doped sol-gel under mild temperature conditions. At least in certain instances reaction and drying will desirably be effected in an oxygen-starved environment. The metal of the sol-gel material functions, when irradiated, to produce a plasmon field for interaction with molecules of an analyte in contact therewith, increasing by orders of magnitude Raman photons that are generate by excitation radiation, and the method allows matching of the metal and metal particle size to a wavelength of light (or incident radiation, e.g., laser radiation) to generate surface plasmons. The porosity of the sol-gel material dramatically increases the surface area, and thereby the amount of metal exposed for analyte interaction. The sensors provided may be in the form of glass vials, fiber optics, multi-well micro-sample plates, etc., having surface coatings of the doped sol-gel material, to provide sampling systems for use in a Raman instrument.

Abstract translation: 适用于痕量化学检测的表面增强拉曼光谱分析传感器的金属掺杂溶胶凝胶材料是通过在温和的温度条件下进行掺杂溶胶 - 凝胶的凝胶化和溶剂去除来生产的。 至少在某些情况下，反应和干燥将理想地在缺氧环境中进行。 溶胶 - 凝胶材料的金属在被照射时起作用以产生与其分子的分子相互作用的等离子体场，增加通过激发辐射产生的拉曼光子数量级，并且该方法允许 金属和金属颗粒尺寸变为波长（或入射辐射，例如激光辐射））以产生表面等离子体激元。 溶胶 - 凝胶材料的孔隙率显着增加了表面积，从而显着地增加了暴露于分析物相互作用的金属的量。 提供的传感器可以是具有掺杂溶胶 - 凝胶材料的表面涂层的玻璃小瓶，光纤，多孔微样品板等的形式，以提供用于拉曼仪器的采样系统。

公开(公告)号：US20240416337A1

公开(公告)日：2024-12-19

申请号：US18742202

申请日：2024-06-13

Applicant: Stuart Farquharson ,  Chetan Shende ,  Duncan Farquharson

Inventor： Stuart Farquharson ,  Chetan Shende ,  Duncan Farquharson

IPC: B01L3/00 ,  G01N33/53 ,  G01N33/543 ,  G01N33/569

Abstract: The present invention provides a novel method and apparatus that employs a signal-enhancing probe in combination with a flow assay strip to bind a target analyte with substantial selectivity, sensitivity, speed, and convenience, through the use of target analyte-specific binding agents and smartphone camera measurement and analysis. The present invention is advantageous for detecting, identifying, quantifying, and analyzing target analytes, such as chemical, biochemical, or biological substances, in analyte samples. The present invention is most effective in detecting, identifying, quantifying, and analyzing drugs, bacterial and viral pathogens in body fluids, such as blood, blood plasma, exhaled breath, nasopharyngeal mucus, and saliva.