公开(公告)号：US12117439B2

公开(公告)日：2024-10-15

申请号：US18503564

申请日：2023-11-07

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50 ,  G01N1/30

CPC classification number: G01N33/5308 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/5047 ,  G01N2001/307 ,  G01N2333/948

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US12241890B2

公开(公告)日：2025-03-04

申请号：US17131174

申请日：2020-12-22

Applicant: 10X Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: C12N15/11 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50 ,  G01N33/53 ,  G01N1/30

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US20250003956A1

公开(公告)日：2025-01-02

申请号：US18828673

申请日：2024-09-09

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N1/30 ,  G01N33/50

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US20240125772A1

公开(公告)日：2024-04-18

申请号：US18503564

申请日：2023-11-07

Applicant: 10x Genomics, Inc.

Inventor： Joshua Delaney ,  Shalini Gohil ,  Jill Herschleb ,  Adam Lowe ,  Albert Kim ,  Meiliana Tjandra

IPC: G01N33/53 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/50

CPC classification number: G01N33/5308 ,  C07D213/04 ,  C07D239/24 ,  C12N9/48 ,  G01N33/5047 ,  G01N2001/307 ,  G01N2333/948

Abstract: The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

公开(公告)号：US20230220368A1

公开(公告)日：2023-07-13

申请号：US17938562

申请日：2022-10-06

Applicant: 10x Genomics, Inc.

Inventor： Albert Kim

IPC: C12N9/64 ,  A01N1/02

CPC classification number: C12N9/6408 ,  C12Y304/21 ,  A01N1/0205

Abstract: The present disclosure provides methods of preparing a fixed biological sample for use in an assay, wherein the method includes treatment of the sample with a low temperature active protease, optionally, in combination with an un-fixing reagent. The disclosure also provides assay methods, include partition-based methods, for fixed biological sample that use the low temperature protease treatment in combination with an un-fixing reagent. Kits comprising protease compositions, un-fixing agent compositions, and other assay reagents for use in the methods are also provided.

公开(公告)号：US20220326251A1

公开(公告)日：2022-10-13

申请号：US17738808

申请日：2022-05-06

Applicant: 10x Genomics, Inc.

Inventor： Cedric Uytingco ,  James Michael Chell ,  Marlon Stoeckius ,  Albert Kim ,  Dulce Ovando Morales ,  Andrej Hartnett ,  Yifeng Yin ,  Jennifer Chew

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6804 ,  G01N33/58 ,  C12Q1/6874 ,  G01N1/30 ,  G01N21/64

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.

公开(公告)号：US20240230483A1

公开(公告)日：2024-07-11

申请号：US18541542

申请日：2023-12-15

Applicant: 10x Genomics, Inc.

Inventor： Jawad N. ABOUSOUD ,  Sharmila Chatterjee Bhattacharjee ,  Albert Kim ,  Ryan Stott ,  Joaquin Trosper-Torres ,  Fernandino Valdecanas

IPC: G01N1/28 ,  C12N9/64 ,  C12Q1/6806 ,  G01N1/30

CPC classification number: G01N1/286 ,  C12N9/6491 ,  C12Q1/6806 ,  G01N1/30 ,  G01N2001/2873 ,  G01N2001/305

Abstract: The present disclosure provides methods for biological sample preparation and analysis that use a chopping and fixing treatment in the preparation of dissociated fixed cells from biological tissue for use in either bulk assays, or in single-cell/single-nucleus assays, such as partition-based assays. The disclosure also provides assay methods, including partition-based methods, for use of the fixed cells prepared from biological tissue that can optionally be used in combination with un-fixing treatments. Kits comprising dissociation reagents, un-fixing agents, and other assay reagents for use in the methods are also provided.

公开(公告)号：US11592447B2

公开(公告)日：2023-02-28

申请号：US17738808

申请日：2022-05-06

Applicant: 10x Genomics, Inc.

Inventor： Cedric Uytingco ,  James Michael Chell ,  Marlon Stoeckius ,  Albert Kim ,  Dulce Ovando Morales ,  Andrej Hartnett ,  Yifeng Yin ,  Jennifer Chew

IPC: G01N33/68 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6874 ,  G01N1/30 ,  G01N21/64 ,  G01N33/58

Abstract: Provided herein are methods for preparing biological samples for spatial proteomic analysis, methods of determining a location of a protein analyte in a biological sample, and methods of determining a location of a protein analyte and a nucleic acid analyte in a biological sample.