公开(公告)号：US20240254552A1

公开(公告)日：2024-08-01

申请号：US18412111

申请日：2024-01-12

Applicant: 10x Genomics, Inc.

Inventor： Katherine PFEIFFER ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund ,  Jawad N. Abousoud ,  Sharmila Chatterjee Bhattacharjee ,  Ryan Stott

IPC: C12Q1/6874 ,  C12Q1/6818

CPC classification number: C12Q1/6874 ,  C12Q1/6818 ,  C12Q2600/16

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20240230483A1

公开(公告)日：2024-07-11

申请号：US18541542

申请日：2023-12-15

Applicant: 10x Genomics, Inc.

Inventor： Jawad N. ABOUSOUD ,  Sharmila Chatterjee Bhattacharjee ,  Albert Kim ,  Ryan Stott ,  Joaquin Trosper-Torres ,  Fernandino Valdecanas

IPC: G01N1/28 ,  C12N9/64 ,  C12Q1/6806 ,  G01N1/30

CPC classification number: G01N1/286 ,  C12N9/6491 ,  C12Q1/6806 ,  G01N1/30 ,  G01N2001/2873 ,  G01N2001/305

Abstract: The present disclosure provides methods for biological sample preparation and analysis that use a chopping and fixing treatment in the preparation of dissociated fixed cells from biological tissue for use in either bulk assays, or in single-cell/single-nucleus assays, such as partition-based assays. The disclosure also provides assay methods, including partition-based methods, for use of the fixed cells prepared from biological tissue that can optionally be used in combination with un-fixing treatments. Kits comprising dissociation reagents, un-fixing agents, and other assay reagents for use in the methods are also provided.

公开(公告)号：US20240019422A1

公开(公告)日：2024-01-18

申请号：US18370826

申请日：2023-09-20

Applicant: 10X GENOMICS, INC.

Inventor： Katherine Pfeiffer ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund ,  Jawad N. Abousoud ,  Sharmila Chatterjee Bhattacharjee ,  Ryan Stott

IPC: G01N33/532 ,  C07K14/74 ,  C12N15/10 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/50 ,  G01N33/53 ,  G01N33/543 ,  G01N33/548 ,  G01N33/569 ,  G01N33/58

CPC classification number: G01N33/532 ,  C07K14/70539 ,  C12N15/1037 ,  C12N15/1055 ,  C12N15/1065 ,  C12N15/1075 ,  C12N15/11 ,  C12N15/85 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6881 ,  G01N33/5032 ,  G01N33/505 ,  G01N33/5304 ,  G01N33/5306 ,  G01N33/5308 ,  G01N33/54306 ,  G01N33/54366 ,  G01N33/548 ,  G01N33/56977 ,  G01N33/58 ,  C12N2310/20 ,  C12N2320/10 ,  C12Q2537/164 ,  C12Q2563/179 ,  C12Q2563/185 ,  C12Q2565/1015 ,  C40B30/04

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.

公开(公告)号：US20240002914A1

公开(公告)日：2024-01-04

申请号：US18217310

申请日：2023-06-30

Applicant: 10x Genomics, Inc.

Inventor： Katherine Pfeiffer ,  Andrew Scott Kohlway ,  Andrew John Hill ,  Paul Eugene Lund ,  Jawad N. Abousoud ,  Sharmila Chatterjee Bhattacharjee ,  Ryan Stott

IPC: C12Q1/6841 ,  G01N1/30

CPC classification number: C12Q1/6841 ,  G01N1/30

Abstract: Provided herein are systems and methods for processing biomolecules (e.g., nucleic acid molecules, proteins) from a sample. A method for processing biomolecules may comprise hybridizing a probe molecule to a target region of a nucleic acid molecule (e.g., a ribonucleic acid (RNA) molecule) and barcoding the probe-nucleic acid molecule complex or derivatives thereof. Such a method can comprise performing a nucleic acid reaction, e.g., extension, denaturation, and amplification. A method for processing a sample may comprise hybridizing probes to (i) target regions of a nucleic acid molecule (e.g., RNA molecule) and (ii) a reporter oligonucleotide of a feature binding group, and barcoding the probe-associated molecules. One or more processes of the methods described herein may be performed within a partition, such as a droplet or well.