公开(公告)号：US20240076656A1

公开(公告)日：2024-03-07

申请号：US18215296

申请日：2023-06-28

申请人： 10x Genomics, Inc.

发明人： David Michael PATTERSON ,  Steven William SHORT ,  Dieter WILK ,  Pingwei ZHUANG ,  Solongo Batjargal ZIRALDO

IPC分类号： C12N15/10 ,  B01J19/00 ,  B41M5/24 ,  C12N9/12 ,  C12N9/22 ,  G03F7/11

CPC分类号： C12N15/1093 ,  B01J19/0046 ,  B41M5/24 ,  C12N9/1264 ,  C12N9/22 ,  C12Y207/07031 ,  C12Y301/11001 ,  G03F7/11 ,  B01J2219/00855 ,  B01J2219/00927 ,  B01J2219/00993 ,  B01J2219/0894 ,  B01J2540/68

摘要： Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate comprising a step of blocking or ablating oligonucleotide molecules in a boundary region separating a plurality of spot regions, and attaching oligonucleotides to oligonucleotide molecules in a first one or more of the spot regions.

公开(公告)号：US20240026444A1

公开(公告)日：2024-01-25

申请号：US18342906

申请日：2023-06-28

申请人： 10x Genomics, Inc.

发明人： Steven William SHORT ,  Dieter WILK

IPC分类号： C12Q1/6874 ,  G03F7/039 ,  G03F7/16 ,  G03F7/20

CPC分类号： C12Q1/6874 ,  G03F7/039 ,  G03F7/162 ,  G03F7/2006 ,  G03F7/2018

摘要： The present disclosure relates in some aspects to methods and compositions for manufacturing molecular arrays using a hybrid approach comprising using non-contact printing (e.g., using inkjet printing, slot die coating, and/or blade coating) and photolithography-guided oligonucleotide hybridization and ligation. In particular, the molecular arrays can be used for determining spatial patterns of abundance and/or expression of a biological target in a sample.

公开(公告)号：US20220228210A1

公开(公告)日：2022-07-21

申请号：US17565047

申请日：2021-12-29

申请人： 10x Genomics, Inc.

发明人： David Michael PATTERSON ,  Denis PRISTINSKI ,  Preyas SHAH ,  Steven William SHORT ,  Dieter WILK ,  Siyuan XING

IPC分类号： C12Q1/6874 ,  C12N15/10

摘要： Provided in some aspects are methods for light-controlled in situ surface patterning of a substrate. Compositions such as nucleic acid arrays produced by the methods are also disclosed. In some embodiments, a method disclosed herein comprises using photoresist for photocontrollable hybridization and/or ligation of nucleic acid molecules, wherein photoresist removal allows hybridization and/or ligation of nucleic acid molecules at the exposed area. A large diversity of barcodes can be created in molecules on the substrate via sequential rounds of light exposure, hybridization, and ligation.